[3dem] Fall 2024 Tomography and Cryo-FIB Workshop at UW-Madison — September 23-27, 2024

[CORINA ELIZABETH EVANS]

REGISTRATION

In-person space is limited, so we encourage you to apply at your earliest convenience. For more details and registration, please visit our workshop page<https://urldefense.com/v3/__https:/explore.wisc.edu/LP=2218__;!!Mih3wA!EAPaNVbgYtTIIhW6C9Nj8TmKSnwa0F3910vTov08uyCN9PJVY5OqOxvCzXoPjLJsAiBbi3dDk65y73-kYXMM-A$> –  Registration closes August 1st, 2024.


Tomography and Cryo-FIB Workshop 2024
Midwest Center for Cryo-Electron Tomography
This workshop will help you learn the fundamentals of cryo-electron tomography (cryo-ET) including sample preparation, FIB-milling, correlative imaging, and cryo-ET data collection and analysis.
[cid:image002.png at 01DAD7A0.F5A6CEE0]Image courtesy of Dr. Jae Yang, PhD

Dear 3dem Readers,

We are thrilled to announce the Fall 2024 Tomography and Cryo-FIB Workshop hosted by the Midwest Center for Cryo-Electron Tomography (MCCET) at UW-Madison. The workshop will be held from September 23-27, 2024. In addition to our MCCET team, we have an exciting group of external speakers that includes:

Claudia
Lopez

—
Haridas Pudavar
—
Lauren Ann Metskas
—
Louise Bertrand
—
(Pacific Northwest Cryo-EM Center)
(Leica)
(Purdue)
(Leica)
Matt
Swulius
—
Sergey Suslov
—
Zoltan Metlagel
—
Zunlong
Ke
—
(Penn State – Hershey)
(TFS)
(TFS)
(UT-Austin)
*Other speakers to be confirmed.

Training topics will include:

  *   Introduction to cryo-ET including the basic principles of dose tolerance, illumination, and tilt series collection parameters.
  *   Training in data acquisition workflows that include screening, data collection, and flexible scripting.
  *   Training in basic data processing including pre-processing, tomogram generation, and considerations for segmentation and sub-tomogram averaging.
  *   Instruction in 2D/3D correlative imaging workflows using the Leica widefield and cryo-confocal microscopes, and the integrated fluorescence microscope in the Aquilos 2.
  *   Guidance in FIB-milling with AutoTEM and advanced FIB workflows such as waffle milling and lift-out methods.

The morning theory sessions of the workshop will be hybrid and the afternoon practical sessions will be in-person only.
Kind regards,

Corina

Corina Elizabeth Evans
Administrative Assistant II
D: (608) 262-7175
E: ceevans3 at wisc.edu<mailto:ceevans3 at wisc.edu>
E: cryoem at biochem.wisc.edu<mailto:CRYOEM at BIOCHEM.WISC.EDU> | https://urldefense.com/v3/__https://cryoem.wisc.edu__;!!Mih3wA!AZgo2HE3DA_QrMuE2-DvyyQgKw58zPkJ4uo4FL9iXVp55Zfvu8mrtBtGOUbaEBedkhk9crGpyzOR1N91dFBNAw$ <https://urldefense.com/v3/__HTTPS://CRYOEM.WISC.EDU__;!!Mih3wA!AZgo2HE3DA_QrMuE2-DvyyQgKw58zPkJ4uo4FL9iXVp55Zfvu8mrtBtGOUbaEBedkhk9crGpyzOR1N_9DiTIsg$ >

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QUESTIONS?

Contact Us

For inquiries about meeting content, registration, and local lodging, please direct your questions to the following three contacts or click here to Email Us<mailto:cotten at wisc.edu;ceevans3 at wisc.edu;cryoem at biochem.wisc.edue>:

Alex Cotten
cotten at wisc.edu<mailto:cotten at wisc.edu>

Corina Evans
ceevans3 at wisc.edu<mailto:ceevans3 at wisc.edu>

UW-Madison Cryo-EM
cryoem at biochem.wisc.edu<mailto:cryoem at biochem.wisc.edu>
We look forward to welcoming you to this enriching event!




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