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<b><span style="font-size:8.0pt;font-family:"Aptos Display",sans-serif;color:black"><br>
</span></b><b><span style="font-size:18.0pt;font-family:"Aptos Display",sans-serif;color:#0E2841">REGISTRATION</span></b><o:p></o:p></p>
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<span style="font-size:11.0pt;line-height:150%;font-family:"Arial",sans-serif;color:#212121">In-person space is limited, so we encourage you to apply at your earliest convenience. For more details and registration, please visit our<span class="apple-converted-space"> </span><b><a href="https://urldefense.com/v3/__https:/explore.wisc.edu/LP=2218__;!!Mih3wA!EAPaNVbgYtTIIhW6C9Nj8TmKSnwa0F3910vTov08uyCN9PJVY5OqOxvCzXoPjLJsAiBbi3dDk65y73-kYXMM-A$" target="_blank" title="https://urldefense.com/v3/__https:/explore.wisc.edu/LP=2218__;!!Mih3wA!EAPaNVbgYtTIIhW6C9Nj8TmKSnwa0F3910vTov08uyCN9PJVY5OqOxvCzXoPjLJsAiBbi3dDk65y73-kYXMM-A$"><span style="color:#0078D7">workshop page</span></a></b><span class="apple-converted-space"> </span>–<span class="apple-converted-space"> </span><b><i>Registration
closes August 1st, 2024</i></b><i>.</i></span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:black"> </span><o:p></o:p></p>
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<p class="MsoNormal"><b><span style="font-size:26.0pt;font-family:"Aptos Display",sans-serif;color:#0E2841">Tomography and Cryo-FIB Workshop 2024</span></b><o:p></o:p></p>
<h1 style="margin-bottom:4.0pt"><span style="font-size:12.0pt;font-family:"Arial",sans-serif;color:#0479A8">Midwest Center for Cryo-Electron Tomography</span><o:p></o:p></h1>
<p class="MsoNormal" style="margin-bottom:8.0pt;line-height:12.0pt;font-variant-caps:normal;orphans:auto;text-align:start;widows:auto;word-spacing:0px">
<span style="font-family:"Arial",sans-serif;color:#212121">This workshop will help you learn the fundamentals of cryo-electron tomography (cryo-ET) including sample preparation, FIB-milling, correlative imaging, and cryo-ET data collection and analysis.</span><o:p></o:p></p>
<p class="MsoNormal" align="right" style="margin-bottom:8.0pt;text-align:right;line-height:12.0pt">
<span style="font-size:10.0pt;color:#0E2841"><img border="0" width="385" height="138" style="width:4.0104in;height:1.4375in" id="Picture_x0020_6" src="cid:image002.png@01DAD7A0.F5A6CEE0"></span><i><span style="font-size:7.5pt;font-family:"Arial",sans-serif;color:#747474">Image
courtesy of Dr. Jae Yang, PhD</span></i><o:p></o:p></p>
<p class="MsoNormal"><span style="font-family:"Arial",sans-serif"> </span><o:p></o:p></p>
<p class="MsoNormal" style="line-height:11.5pt"><span style="font-family:"Arial",sans-serif;color:#212121">Dear 3dem Readers,</span><o:p></o:p></p>
<p class="MsoNormal" style="line-height:11.5pt;font-variant-caps:normal;orphans:auto;text-align:start;widows:auto;word-spacing:0px">
<span style="font-family:"Arial",sans-serif;color:#212121"> </span><o:p></o:p></p>
<p class="MsoNormal" style="line-height:11.5pt"><span style="font-family:"Arial",sans-serif;color:#212121">We are thrilled to announce the Fall 2024 Tomography and Cryo-FIB Workshop hosted by the Midwest Center for Cryo-Electron Tomography (MCCET) at UW-Madison.
The workshop will be held from September 23-27, 2024. In addition to our MCCET team, we have an exciting group of external</span><span style="font-family:"Arial",sans-serif">
<span style="color:#212121">speakers that includes:</span></span><o:p></o:p></p>
<p class="MsoNormal" style="line-height:11.5pt"> <o:p></o:p></p>
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<b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:black">Claudia<br>
Lopez</span></b><o:p></o:p></p>
<p class="MsoListParagraph"><span style="color:#C5050B">—</span><o:p></o:p></p>
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<p class="MsoNormal" align="center" style="text-align:center;line-height:11.5pt">
<b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#212121">Haridas</span></b><b><span style="font-size:10.0pt;font-family:"Arial",sans-serif">
<span class="spelle0"><span style="color:#212121">Pudavar</span></span></span></b><o:p></o:p></p>
<p class="MsoNormal" align="center" style="text-align:center;line-height:11.5pt">
<span style="font-family:"Arial",sans-serif;color:#C5050B">—</span><o:p></o:p></p>
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<td width="90" style="width:67.5pt;border-top:solid #0479A8 1.0pt;border-left:none;border-bottom:none;border-right:solid #0479A8 1.0pt;background:#F7F7F7;padding:0in 5.4pt 0in 5.4pt">
<p class="MsoNormal" align="center" style="text-align:center;line-height:11.5pt">
<b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#212121">Lauren Ann</span></b><b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:black">
</span></b><span class="spelle0"><b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#212121">Metskas</span></b></span><o:p></o:p></p>
<p class="MsoNormal" align="center" style="text-align:center;line-height:11.5pt">
<span style="font-family:"Arial",sans-serif;color:#C5050B">—</span><o:p></o:p></p>
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<td width="96" style="width:72.2pt;border-top:solid #0479A8 1.0pt;border-left:none;border-bottom:none;border-right:solid #0479A8 1.0pt;padding:0in 5.4pt 0in 5.4pt">
<p class="MsoNormal" align="center" style="text-align:center;line-height:11.5pt">
<b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#212121">Louise Bertrand</span></b><o:p></o:p></p>
<p class="MsoNormal" align="center" style="text-align:center;line-height:11.5pt">
<span style="font-family:"Arial",sans-serif;color:#C5050B">—</span><o:p></o:p></p>
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<p class="MsoNormal" align="center" style="text-align:center;line-height:11.5pt">
<span style="font-size:9.0pt;font-family:"Calibri",sans-serif;color:black">(Pacific Northwest Cryo-EM</span><span style="font-family:"Calibri",sans-serif;color:black">
</span><span style="font-size:9.0pt;font-family:"Calibri",sans-serif;color:black">Center)</span><o:p></o:p></p>
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<p class="MsoNormal" align="center" style="text-align:center;line-height:11.5pt">
<span style="font-size:9.0pt">(Leica)</span><o:p></o:p></p>
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<p class="MsoNormal" align="center" style="text-align:center;line-height:11.5pt">
<span style="font-size:9.0pt;font-family:"Calibri",sans-serif;color:#212121">(Purdue)</span><o:p></o:p></p>
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<p class="MsoNormal" align="center" style="text-align:center;line-height:11.5pt">
<span style="font-size:9.0pt">(Leica)</span><o:p></o:p></p>
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<span class="apple-converted-space"><b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#212121">Matt</span></b></span><b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#212121"><br>
<span class="spelle0">Swulius</span></span></b><o:p></o:p></p>
<p class="MsoNormal" align="center" style="text-align:center;line-height:11.5pt">
<span style="font-family:"Arial",sans-serif">—</span><o:p></o:p></p>
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<p class="MsoNormal" align="center" style="text-align:center;line-height:11.5pt">
<b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#212121">Sergey Suslov</span></b><o:p></o:p></p>
<p class="MsoNormal" align="center" style="text-align:center;line-height:11.5pt">
<span style="font-family:"Arial",sans-serif;color:#C5050B">—</span><o:p></o:p></p>
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<td width="90" style="width:67.5pt;border:none;border-right:solid #0479A8 1.0pt;padding:0in 5.4pt 0in 5.4pt">
<p class="MsoNormal" align="center" style="text-align:center;line-height:11.5pt">
<b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#212121">Zoltan</span></b><b><span style="font-size:10.0pt;font-family:"Arial",sans-serif">
<span class="spelle0"><span style="color:#212121">Metlagel</span></span></span></b><o:p></o:p></p>
<p class="MsoNormal" align="center" style="text-align:center;line-height:11.5pt">
<span style="font-family:"Arial",sans-serif;color:#C5050B">—</span><o:p></o:p></p>
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<td width="96" style="width:72.2pt;border:none;border-right:solid #0479A8 1.0pt;background:#F7F7F7;padding:0in 5.4pt 0in 5.4pt">
<p class="MsoNormal" align="center" style="text-align:center;line-height:11.5pt">
<span class="spelle0"><b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#212121">Zunlong</span></b></span><b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:black"><br>
</span></b><b><span style="font-size:10.0pt;font-family:"Arial",sans-serif;color:#212121">Ke</span></b><o:p></o:p></p>
<p class="MsoNormal" align="center" style="text-align:center;line-height:11.5pt">
<span style="font-family:"Arial",sans-serif;color:#C5050B">—</span><o:p></o:p></p>
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<p class="MsoNormal" align="center" style="text-align:center;line-height:11.5pt">
<span style="font-size:9.0pt">(Penn State – Hershey)</span><o:p></o:p></p>
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<p class="MsoNormal" align="center" style="text-align:center;line-height:11.5pt">
<span style="font-size:9.0pt;color:black">(TFS)</span><o:p></o:p></p>
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<p class="MsoNormal" align="center" style="text-align:center;line-height:11.5pt">
<span style="font-size:9.0pt">(TFS)</span><o:p></o:p></p>
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<td width="96" valign="top" style="width:72.2pt;border:none;border-right:solid #0479A8 1.0pt;background:#F7F7F7;padding:0in 5.4pt 0in 5.4pt">
<p class="MsoNormal" align="center" style="text-align:center;line-height:11.5pt">
<span style="font-size:9.0pt;font-family:"Calibri",sans-serif;color:#212121">(UT-Austin</span><span style="font-size:9.0pt;font-family:"Calibri",sans-serif;color:black">)</span><o:p></o:p></p>
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<p class="MsoNormal" style="line-height:11.5pt"><i><span style="font-size:9.0pt;font-family:"Calibri",sans-serif;color:white">*Other speakers to be confirmed.</span></i><o:p></o:p></p>
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<b><span style="font-family:"Arial",sans-serif;color:black">Training topics will include:</span></b><o:p></o:p></p>
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<span style="font-family:"Arial",sans-serif">Introduction to cryo-ET including the basic principles of dose tolerance, illumination, and tilt series collection parameters.<span class="apple-converted-space"> </span></span><o:p></o:p></li><li class="MsoNormal" style="color:black;margin-top:6.0pt;line-height:11.5pt;mso-list:l0 level1 lfo1">
<span style="font-family:"Arial",sans-serif">Training in data acquisition workflows that include screening, data collection, and flexible scripting.</span><o:p></o:p></li><li class="MsoNormal" style="color:black;margin-top:6.0pt;line-height:11.5pt;mso-list:l0 level1 lfo1">
<span style="font-family:"Arial",sans-serif">Training in basic data processing including pre-processing, tomogram generation, and considerations for segmentation and sub-tomogram averaging.<span class="apple-converted-space"> </span></span><o:p></o:p></li><li class="MsoNormal" style="color:black;margin-top:6.0pt;line-height:11.5pt;mso-list:l0 level1 lfo1">
<span style="font-family:"Arial",sans-serif">Instruction in 2D/3D correlative imaging workflows using the Leica widefield and cryo-confocal microscopes, and the integrated fluorescence microscope in the Aquilos 2.<span class="apple-converted-space"> </span></span><o:p></o:p></li><li class="MsoNormal" style="color:black;margin-top:6.0pt;line-height:11.5pt;mso-list:l0 level1 lfo1">
<span style="font-family:"Arial",sans-serif">Guidance in FIB-milling with<span class="apple-converted-space"> </span></span><span class="spelle0">AutoTEM</span><span class="apple-converted-space"><span style="font-family:"Arial",sans-serif"> </span></span><span style="font-family:"Arial",sans-serif">and
advanced FIB workflows such as waffle milling and lift-out methods.<span class="apple-converted-space"> </span></span><o:p></o:p></li></ul>
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<span style="font-size:11.0pt;font-family:"Arial",sans-serif;color:black;background:white">The morning theory sessions of the workshop will be hybrid and the afternoon practical sessions will be in-person only.</span><o:p></o:p></p>
<p class="MsoNormal"><span style="font-family:"Arial",sans-serif;color:#212121">Kind regards,</span><o:p></o:p></p>
<p class="MsoNormal"><span style="color:#212121"> </span><o:p></o:p></p>
<p class="MsoNormal"><i><span style="font-size:12.0pt;font-family:"Segoe UI Semilight",sans-serif;color:black">Corina</span></i><o:p></o:p></p>
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<p class="MsoNormal" style="margin-bottom:2.0pt"><b><span style="font-family:"Arial",sans-serif;color:black">Corina Elizabeth Evans</span></b><o:p></o:p></p>
<p class="MsoNormal" style="margin-bottom:2.0pt"><i><span style="font-family:"Arial",sans-serif;color:black">Administrative Assistant II</span></i><o:p></o:p></p>
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<p class="MsoNormal" style="margin-bottom:2.0pt"><span style="font-family:"Arial",sans-serif;color:#212121">D: (608) 262-7175</span><o:p></o:p></p>
<p class="MsoNormal" style="margin-bottom:2.0pt"><span style="font-family:"Arial",sans-serif;color:black">E: </span><span style="color:#212121"><a href="mailto:ceevans3@wisc.edu" title="mailto:ceevans3@wisc.edu"><span style="font-family:"Arial",sans-serif;color:#0078D7">ceevans3@wisc.edu</span></a></span><o:p></o:p></p>
<p class="MsoNormal" style="margin-bottom:2.0pt"><span style="font-family:"Arial",sans-serif;color:black">E:<b> </b></span><span style="color:#212121"><a href="mailto:CRYOEM@BIOCHEM.WISC.EDU" title="mailto:CRYOEM@BIOCHEM.WISC.EDU"><span style="font-family:"Arial",sans-serif;color:#0078D7">cryoem@biochem.wisc.edu</span></a></span><span style="font-family:"Arial",sans-serif;color:#0086F0"> </span><b><span style="font-family:"Arial",sans-serif;color:#C5050C">| </span></b><span style="color:#212121"><a href="https://urldefense.com/v3/__HTTPS://CRYOEM.WISC.EDU__;!!Mih3wA!AZgo2HE3DA_QrMuE2-DvyyQgKw58zPkJ4uo4FL9iXVp55Zfvu8mrtBtGOUbaEBedkhk9crGpyzOR1N_9DiTIsg$" title="HTTPS://CRYOEM.WISC.EDU"><span style="font-family:"Arial",sans-serif;color:#0078D7">https://cryoem.wisc.edu</span></a></span><o:p></o:p></p>
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<p class="answer0" align="center" style="margin-bottom:7.0pt;text-align:center"><b><span style="font-size:14.0pt;font-family:"Aptos Display",sans-serif;color:black">QUESTIONS?</span></b><o:p></o:p></p>
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<b><span style="font-size:14.0pt;font-family:"Aptos Display",sans-serif;color:#C5050B">Contact Us</span></b><o:p></o:p></p>
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<span style="font-size:11.0pt;font-family:"Arial",sans-serif;color:#212121">For inquiries about meeting content, registration, and local lodging, please direct your questions to the following three contacts or click here to</span><span class="apple-converted-space"><span style="font-family:"Arial",sans-serif;color:#0E2841"> </span></span><b><span style="font-size:11.0pt;font-family:"Arial",sans-serif;color:#212121"><a href="mailto:cotten@wisc.edu;ceevans3@wisc.edu;cryoem@biochem.wisc.edue" title="mailto:cotten@wisc.edu;ceevans3@wisc.edu;cryoem@biochem.wisc.edue"><span style="color:#0078D7">Email Us</span></a></span></b><span style="font-size:11.0pt;font-family:"Arial",sans-serif;color:#212121">:</span><o:p></o:p></p>
<p align="center" style="margin-bottom:.25in;text-align:center"><b><span style="font-size:11.0pt;font-family:"Arial",sans-serif;color:#212121">Alex Cotten</span></b><span style="font-size:11.0pt;font-family:"Arial",sans-serif;color:#212121"><br>
<a href="mailto:cotten@wisc.edu" title="mailto:cotten@wisc.edu"><span style="color:#0078D7">cotten@wisc.edu</span></a></span><o:p></o:p></p>
<p align="center" style="margin-bottom:.25in;text-align:center"><b><span style="font-size:11.0pt;font-family:"Arial",sans-serif;color:#212121">Corina Evans</span></b><span style="font-size:11.0pt;font-family:"Arial",sans-serif;color:#212121"><br>
</span><span style="font-size:11.0pt;font-family:"Arial",sans-serif;color:#0070C0"><a href="mailto:ceevans3@wisc.edu"><span style="color:#0070C0">ceevans3@wisc.edu</span></a></span><o:p></o:p></p>
<p align="center" style="margin-bottom:.25in;text-align:center"><b><span style="font-size:11.0pt;font-family:"Arial",sans-serif;color:#212121">UW-Madison Cryo-EM<br>
</span></b><span style="font-size:11.0pt;font-family:"Arial",sans-serif;color:#212121"><a href="mailto:cryoem@biochem.wisc.edu" title="mailto:cryoem@biochem.wisc.edu"><span style="color:#0078D7">cryoem@biochem.wisc.edu</span></a></span><o:p></o:p></p>
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<span style="font-family:"Arial",sans-serif;color:#212121">We look forward to welcoming you to this enriching event<b><i>!</i></b></span><o:p></o:p></p>
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