[3dem] RELION low_resol_join_halves parameter
Craig Yoshioka
yoshiokc at ohsu.edu
Tue Dec 12 09:07:41 PST 2017
I’ve started the job in the GUI, killed it, then rerun from the CLI with the same output (change the output dir to match). Easiest way I’ve found to do it if you don’t want the GUI to lose track of the job.
On Dec 12, 2017, at 9:02 AM, Buecker, Robert <robert.buecker at mpsd.mpg.de<mailto:robert.buecker at mpsd.mpg.de>> wrote:
Dear list,
I am currently working on a single-particle reconstruction in RELION at unusually low resolutions (worse than 100A).
I noticed, that when running a 3D auto-refine job through the GUI, it will supply a "--low_resol_join_halves 40"-option to relion_refine_mpi, which cannot seem to be overriden. However, due to the low resolution of our data, I'd need that parameter to be significantly higher (at 40, my reported FSCs remain at 1 up to a sharp cutoff, which is expected as the FSC is from a joined set up to almost the resolution). Does anybody know a workaround, apart from not using the GUI at all?
Thanks in advance and best wishes from Hamburg,
Robert
--
dr. robert bücker
max planck institute for the structure and dynamics of matter
atomically resolved dynamics department
desy bldg. 99 (cfel)
luruper chaussee 149
22761 hamburg, germany
tel. +49 40 8998-6278 / -86043
mob. +49 157 70210628
robert.buecker at mpsd.mpg.de<mailto:robert.buecker at mpsd.mpg.de>
http://www.mpsd.mpg.de/68786/Femtosecond_Diffraction
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