[3dem] RELION low_resol_join_halves parameter
Buecker, Robert
robert.buecker at mpsd.mpg.de
Tue Dec 12 09:02:27 PST 2017
Dear list,
I am currently working on a single-particle reconstruction in RELION at unusually low resolutions (worse than 100A).
I noticed, that when running a 3D auto-refine job through the GUI, it will supply a "--low_resol_join_halves 40"-option to relion_refine_mpi, which cannot seem to be overriden. However, due to the low resolution of our data, I'd need that parameter to be significantly higher (at 40, my reported FSCs remain at 1 up to a sharp cutoff, which is expected as the FSC is from a joined set up to almost the resolution). Does anybody know a workaround, apart from not using the GUI at all?
Thanks in advance and best wishes from Hamburg,
Robert
--
dr. robert bücker
max planck institute for the structure and dynamics of matter
atomically resolved dynamics department
desy bldg. 99 (cfel)
luruper chaussee 149
22761 hamburg, germany
tel. +49 40 8998-6278 / -86043
mob. +49 157 70210628
robert.buecker at mpsd.mpg.de
http://www.mpsd.mpg.de/68786/Femtosecond_Diffraction
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