[3dem] RELION low_resol_join_halves parameter

[Sjors Scheres]

Hi Robert,
If you replace the queue submission command entry on the GUI from 'qsub' 
(or whatever you use) to 'cat', the submission script gets written out 
and the directory structure created, but the job will not get submitted 
to the queue. You can then edit the script and submit it from the 
command line. Alternatively, if you are on a local system, you can use 
the print-command button and edit the result of that.
HTH,
Sjors

On 12/12/2017 05:02 PM, Buecker, Robert wrote:
> Dear list,
>
> I am currently working on a single-particle reconstruction in RELION at unusually low resolutions (worse than 100A).
> I noticed, that when running a 3D auto-refine job through the GUI, it will supply a "--low_resol_join_halves 40"-option to relion_refine_mpi, which cannot seem to be overriden. However, due to the low resolution of our data, I'd need that parameter to be significantly higher (at 40, my reported FSCs remain at 1 up to a sharp cutoff, which is expected as the FSC is from a joined set up to almost the resolution). Does anybody know a workaround, apart from not using the GUI at all?
>
> Thanks in advance and best wishes from Hamburg,
> Robert
>
> --
> dr. robert bücker
>   max planck institute for the structure and dynamics of matter
>   atomically resolved dynamics department
>   desy bldg. 99 (cfel)
>   luruper chaussee 149
>   22761 hamburg, germany
>   tel. +49 40 8998-6278 / -86043
>   mob. +49 157 70210628
>
>   robert.buecker at mpsd.mpg.de
>   http://www.mpsd.mpg.de/68786/Femtosecond_Diffraction
>
>
>
>
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-- 
Sjors Scheres
MRC Laboratory of Molecular Biology
Francis Crick Avenue, Cambridge Biomedical Campus
Cambridge CB2 0QH, U.K.
tel: +44 (0)1223 267061
http://www2.mrc-lmb.cam.ac.uk/groups/scheres