[3dem] This is my question to all the members experts and non-experts who are working on membrane proteins in the CryoEM

[Carlos HENRIQUE FERNANDES]

Hi, 
I just wanna do a correction in my last message. 
The channel structure of VCCN1 that I pointed is a homopentamer. The MW recorded (43 kDa) is for 1 subunit, so this actually 210 kDA. 
Sorry for my mistake. 
Best regards, 
Carlos. 


From: "Carlos HENRIQUE FERNANDES" <carlos.henrique_fernandes at sorbonne-universite.fr> 
To: "anindadutta86" <anindadutta86 at gmail.com> 
Cc: "3dem" <3dem at ncmir.ucsd.edu> 
Sent: Monday, April 8, 2024 2:09:35 PM 
Subject: Re: [3dem] This is my question to all the members experts and non-experts who are working on membrane proteins in the CryoEM 

Hi Aninda, 
Complementing the suggestions provided by Guillaume, recently it as published a review regarding the most critical parameters for sample preparation of membrane proteins for cryo-EM: [ https://urldefense.com/v3/__https://www.mdpi.com/1422-0067/24/19/14785*B47-ijms-24-14785__;Iw!!Mih3wA!HfnQmpHunTTMlLYh3cpbQSN_yvnyjZ-1YuqzYUNF2vmOh_cqwF3U-s4LumRduYEkxJvft15TKOMGS0XWZdQncT-kMjb39QhZddDlX9EEcw$  | https://urldefense.com/v3/__https://www.mdpi.com/1422-0067/24/19/14785*B47-ijms-24-14785__;Iw!!Mih3wA!HfnQmpHunTTMlLYh3cpbQSN_yvnyjZ-1YuqzYUNF2vmOh_cqwF3U-s4LumRduYEkxJvft15TKOMGS0XWZdQncT-kMjb39QhZddDlX9EEcw$ . ] 
In this review, is reported that for some proteins under 100 kDa, nanodiscs could be very useful to overcome size-related limitations in the application of cryo-EM SPA for smaller proteins. Moreover, there is a report of a voltage-dependent chloride channel (VCNN1) with a total structure weight of 42 kDa (PDB ID 7EK2) reconstituted in nanodiscs at 2.7 Å resolution: [ https://urldefense.com/v3/__https://www.nature.com/articles/s41467-022-30292-w__;!!Mih3wA!HfnQmpHunTTMlLYh3cpbQSN_yvnyjZ-1YuqzYUNF2vmOh_cqwF3U-s4LumRduYEkxJvft15TKOMGS0XWZdQncT-kMjb39QhZddAIOSnkgQ$  | https://urldefense.com/v3/__https://www.nature.com/articles/s41467-022-30292-w__;!!Mih3wA!HfnQmpHunTTMlLYh3cpbQSN_yvnyjZ-1YuqzYUNF2vmOh_cqwF3U-s4LumRduYEkxJvft15TKOMGS0XWZdQncT-kMjb39QhZddAIOSnkgQ$  ] 
I hope that this could help you. 
Best regards, 
Carlos. 

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