[3dem] This is my question to all the members experts and non-experts who are working on membrane proteins in the CryoEM

[Walsh, Richard]

The channel structure you pointed out VCCN1 is a homopentamer. The MW recorded is for 1 subunit. So this is actually 210 kDA, which is correct in the Map deposition (the calculated) but only lists one chain in the pdb.

Best regards

Richard

Richard M. Walsh , Ph.D.

The Harvard Cryo-EM Center for Structural Biology
Harvard Medical School, 250 Longwood Avenue, SGM 104, Boston, MA 02115
Phone: (617) 432-9042
Email: Richard_Walsh at hms.harvard.edu
Web: cryoem.hms.harvard.edu
________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Carlos HENRIQUE FERNANDES <carlos.henrique_fernandes at sorbonne-universite.fr>
Sent: Monday, April 8, 2024 8:09:35 AM
To: anindadutta86 at gmail.com <anindadutta86 at gmail.com>
Cc: 3dem at ncmir.ucsd.edu <3dem at ncmir.ucsd.edu>
Subject: Re: [3dem] This is my question to all the members experts and non-experts who are working on membrane proteins in the CryoEM

Hi Aninda,
Complementing the suggestions provided by Guillaume, recently it as published a review regarding the most critical parameters for sample preparation of membrane proteins for cryo-EM: https://urldefense.com/v3/__https://www.mdpi.com/1422-0067/24/19/14785*B47-ijms-24-14785__;Iw!!Mih3wA!E544QJHfSIjYYNVS-DgWNkNJLHwQm3bJMoS3zmjmnQQFP-TK0BQ8VnhDTP4oShSH-IywClKS4xlM-Eth6-kkoniK4nAbtyfvzw$ .<https://urldefense.com/v3/__https://www.mdpi.com/1422-0067/24/19/14785*B47-ijms-24-14785__;Iw!!Mih3wA!BMiTj-FflnmlxCXmTEa6DadVq1VB77xp4lk_BMGEQy8fEFUZTA2rR-VDzbAz_w857TlZteJpiCtEXAjm1VkKEL5UFSMKt2iJKA5i1uI6lQ$>
In this review, is reported that for some proteins under 100 kDa, nanodiscs could be very useful to overcome size-related limitations in the application of cryo-EM SPA for smaller proteins. Moreover, there is a report of a voltage-dependent chloride channel (VCNN1) with a total structure weight of 42 kDa (PDB ID 7EK2) reconstituted in nanodiscs at 2.7 Å resolution:  https://urldefense.com/v3/__https://www.nature.com/articles/s41467-022-30292-w__;!!Mih3wA!E544QJHfSIjYYNVS-DgWNkNJLHwQm3bJMoS3zmjmnQQFP-TK0BQ8VnhDTP4oShSH-IywClKS4xlM-Eth6-kkoniK4nAcQmjZMA$ <https://urldefense.com/v3/__https://www.nature.com/articles/s41467-022-30292-w__;!!Mih3wA!BMiTj-FflnmlxCXmTEa6DadVq1VB77xp4lk_BMGEQy8fEFUZTA2rR-VDzbAz_w857TlZteJpiCtEXAjm1VkKEL5UFSMKt2iJKA4BE7zTJA$>
I hope that this could help you.
Best regards,
Carlos.
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20240408/34a9135a/attachment.html>