<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=iso-8859-1">
</head>
<body>
<div dir="ltr">
<div dir="ltr"><span style="text-decoration: none; font-family: Calibri, sans-serif; font-size: 14.666667px; display: inline !important; color: rgb(33, 33, 33);">The channel structure you pointed out VCCN1 is a homopentamer. The MW recorded is for 1 subunit.
So this is actually 210 kDA, which is correct in the Map deposition (the calculated) but only lists one chain in the pdb.</span><br>
</div>
<div dir="ltr"><span style="text-decoration: none; font-family: Calibri, sans-serif; font-size: 14.666667px; display: inline !important; color: rgb(33, 33, 33);"><br>
</span></div>
<div dir="ltr"><span style="text-decoration: none; font-family: Calibri, sans-serif; font-size: 14.666667px; display: inline !important; color: rgb(33, 33, 33);">Best regards</span></div>
<div dir="ltr"><span style="text-decoration: none; font-family: Calibri, sans-serif; font-size: 14.666667px; display: inline !important; color: rgb(33, 33, 33);"><br>
</span></div>
<div dir="ltr"><span style="text-decoration: none; font-family: Calibri, sans-serif; font-size: 14.666667px; display: inline !important; color: rgb(33, 33, 33);">Richard </span></div>
<div id="ms-outlook-mobile-signature">
<div><br>
</div>
<div style="direction:ltr">Richard M. Walsh , Ph.D.</div>
<div><br>
</div>
<div style="direction:ltr">The Harvard Cryo-EM Center for Structural Biology </div>
<div style="direction:ltr">Harvard Medical School, 250 Longwood Avenue, SGM 104, Boston, MA 02115</div>
<div style="direction:ltr"></div>
<div style="direction:ltr">Phone: (617) 432-9042</div>
<div style="direction:ltr">Email: Richard_Walsh@hms.harvard.edu</div>
<div style="direction:ltr">Web: cryoem.hms.harvard.edu</div>
</div>
</div>
<hr style="display:inline-block;width:98%" tabindex="-1">
<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Carlos HENRIQUE FERNANDES <carlos.henrique_fernandes@sorbonne-universite.fr><br>
<b>Sent:</b> Monday, April 8, 2024 8:09:35 AM<br>
<b>To:</b> anindadutta86@gmail.com <anindadutta86@gmail.com><br>
<b>Cc:</b> 3dem@ncmir.ucsd.edu <3dem@ncmir.ucsd.edu><br>
<b>Subject:</b> Re: [3dem] This is my question to all the members experts and non-experts who are working on membrane proteins in the CryoEM</font>
<div> </div>
</div>
<div>
<div style="font-family:arial,helvetica,sans-serif; font-size:12pt; color:#000000">
<div>Hi Aninda,<br data-mce-bogus="1">
</div>
<div><span class="x_HwtZe" lang="en"><span class="x_jCAhz x_ChMk0b"><span class="x_ryNqvb">Complementing the suggestions provided by Guillaume, recently it as published a review regarding the most critical parameters for sample preparation of membrane proteins
for cryo-EM: <a href="https://urldefense.com/v3/__https://www.mdpi.com/1422-0067/24/19/14785*B47-ijms-24-14785__;Iw!!Mih3wA!BMiTj-FflnmlxCXmTEa6DadVq1VB77xp4lk_BMGEQy8fEFUZTA2rR-VDzbAz_w857TlZteJpiCtEXAjm1VkKEL5UFSMKt2iJKA5i1uI6lQ$">
https://www.mdpi.com/1422-0067/24/19/14785#B47-ijms-24-14785.</a><br>
</span></span></span></div>
<div><span class="x_HwtZe" lang="en"><span class="x_jCAhz x_ChMk0b"><span class="x_ryNqvb">In this review, is reported that for some proteins under 100 kDa, nanodiscs could be very useful to overcome size-related limitations in the application of cryo-EM SPA
for smaller proteins. Moreover, there is a report of a </span></span></span><span class="x_HwtZe" lang="en"><span class="x_jCAhz x_ChMk0b"><span class="x_ryNqvb">voltage-dependent chloride channel (VCNN1) with a total structure weight of 42 kDa (PDB ID 7EK2)
reconstituted in nanodiscs at 2.7 Å resolution: <a href="https://urldefense.com/v3/__https://www.nature.com/articles/s41467-022-30292-w__;!!Mih3wA!BMiTj-FflnmlxCXmTEa6DadVq1VB77xp4lk_BMGEQy8fEFUZTA2rR-VDzbAz_w857TlZteJpiCtEXAjm1VkKEL5UFSMKt2iJKA4BE7zTJA$">
https://www.nature.com/articles/s41467-022-30292-w</a> <br>
</span></span></span></div>
<div><span class="x_HwtZe" lang="en"><span class="x_jCAhz x_ChMk0b"><span class="x_ryNqvb">I hope that this could help you.<br data-mce-bogus="1">
</span></span></span></div>
<div><span class="x_HwtZe" lang="en"><span class="x_jCAhz x_ChMk0b"><span class="x_ryNqvb">Best regards,<br data-mce-bogus="1">
</span></span></span></div>
<div><span class="x_HwtZe" lang="en"><span class="x_jCAhz x_ChMk0b"><span class="x_ryNqvb">Carlos.<br data-mce-bogus="1">
</span></span></span></div>
</div>
</div>
</body>
</html>