<html><body><div style="font-family: arial, helvetica, sans-serif; font-size: 12pt; color: #000000">Hi,<br>I just wanna do a correction in my last message.<br>The channel structure of VCCN1 that I pointed is a homopentamer. The MW recorded (43 kDa) is for 1 subunit, so this actually 210 kDA.<br>Sorry for my mistake.<br>Best regards,<br>Carlos.<br><br><hr id="zwchr" data-marker=""><div data-marker=""><b>From: </b>"Carlos HENRIQUE FERNANDES" <carlos.henrique_fernandes@sorbonne-universite.fr><br><b>To: </b>"anindadutta86" <anindadutta86@gmail.com><br><b>Cc: </b>"3dem" <3dem@ncmir.ucsd.edu><br><b>Sent: </b>Monday, April 8, 2024 2:09:35 PM<br><b>Subject: </b>Re: [3dem] This is my question to all the members experts and non-experts who are working on membrane proteins in the CryoEM<br></div><br><div data-marker=""><div style="font-family:'arial' , 'helvetica' , sans-serif;font-size:12pt;color:#000000"><div>Hi Aninda,<br></div><div><span class="HwtZe" lang="en"><span class="jCAhz ChMk0b"><span class="ryNqvb">Complementing the suggestions provided by Guillaume, recently it as published a review regarding the most critical parameters for sample preparation of membrane proteins for cryo-EM: <a href="https://urldefense.com/v3/__https://www.mdpi.com/1422-0067/24/19/14785*B47-ijms-24-14785__;Iw!!Mih3wA!HfnQmpHunTTMlLYh3cpbQSN_yvnyjZ-1YuqzYUNF2vmOh_cqwF3U-s4LumRduYEkxJvft15TKOMGS0XWZdQncT-kMjb39QhZddDlX9EEcw$" target="_blank" rel="nofollow noopener noreferrer">https://www.mdpi.com/1422-0067/24/19/14785#B47-ijms-24-14785.</a><br></span></span></span></div><div><span class="HwtZe" lang="en"><span class="jCAhz ChMk0b"><span class="ryNqvb">In this review, is reported that for some proteins under 100 kDa, nanodiscs could be very useful to overcome size-related limitations in the application of cryo-EM SPA for smaller proteins. Moreover, there is a report of a </span></span></span><span class="HwtZe" lang="en"><span class="jCAhz ChMk0b"><span class="ryNqvb">voltage-dependent chloride channel (VCNN1) with a total structure weight of 42 kDa (PDB ID 7EK2) reconstituted in nanodiscs at 2.7 Å resolution: <a href="https://urldefense.com/v3/__https://www.nature.com/articles/s41467-022-30292-w__;!!Mih3wA!HfnQmpHunTTMlLYh3cpbQSN_yvnyjZ-1YuqzYUNF2vmOh_cqwF3U-s4LumRduYEkxJvft15TKOMGS0XWZdQncT-kMjb39QhZddAIOSnkgQ$" target="_blank" rel="nofollow noopener noreferrer">https://www.nature.com/articles/s41467-022-30292-w</a> <br></span></span></span></div><div><span class="HwtZe" lang="en"><span class="jCAhz ChMk0b"><span class="ryNqvb">I hope that this could help you.<br></span></span></span></div><div><span class="HwtZe" lang="en"><span class="jCAhz ChMk0b"><span class="ryNqvb">Best regards,<br></span></span></span></div><div><span class="HwtZe" lang="en"><span class="jCAhz ChMk0b"><span class="ryNqvb">Carlos.</span></span></span></div></div><br></div></div></body></html>