[3dem] Carbon evaporator for SPA

Gérard Pehau-Arnaudet gpehau at pasteur.fr
Fri Sep 15 02:08:01 PDT 2023


Hello Marion
We have Cressington Turbo Carbon Coater 208 Carbon in Pasteur: we use it
only to carbon coat grids for SPA with rods. We control by eyes the
thickness
We also have the Leica ACE 600 but we only use it to carbon coat the
formvar grids for sections: in my hands the thread never gives me a good
quality of carbon.
Amicalement
Gérard



Le jeu. 14 sept. 2023 à 15:09, Marion Jasnin <
marion.jasnin at helmholtz-munich.de> a écrit :

> Dear Rachel, Eugene and Artemis,
>
> Many thanks for your valuable input too.
>
> The choice is then between the Q150V ES Plus, CCU-010 and the Cressington
> Turbo Carbon Coater 208.
>
> Thanks again!
> Cheers,
> Marion
>
> ------------------------------
> *From:* 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Artemis Kosta <
> akosta at imm.cnrs.fr>
> *Sent:* 14 September 2023 14:10
> *To:* Eugene Pichkur <eugene.pichkur at gmail.com>; Jasnin, Marion <
> jasnin at biochem.mpg.de>
> *Cc:* 3dem at ncmir.ucsd.edu <3dem at ncmir.ucsd.edu>
> *Subject:* Re: [3dem] Carbon evaporator for SPA
>
> We have a Cressington Turbo Carbon Coater 208 Carbon and we are very
> happy also.
> You can combine it with a glow discharge module also.
>
> Leica EM ACE600 didn't work nicely during the demo and I think the
> consumables were quite expensive comparing to the carbon rods for
> Cressington.
>
> Best regards
> Artemis Kosta
>
> Le 14/09/2023 à 14:00, Eugene Pichkur a écrit :
> > Long story short, high vacuum is necessary for fine grains, so a
> > turbo/diffusion pump is a must have.
> >
> > Leica EM ACE600 is not a reliable machine, we have several problems with
> > it, especially with the thickness monitor. I know several other labs
> > where people are also not happy with it for similar reasons.
> > Quorum Q150V is a simple and nice machine, I had no problems with it in
> > several labs and used it for thin carbon film deposition, that was
> > successfully used for SPA work (EMD-10655). I would go with the Q150V ES
> > Plus version just to be able to deposit films onto the surface of
> > samples for SEM. But I haven't managed to get the same quality of the
> > film as in Quantifoil+2nm grids (these things are very-very thin and
> > nice) but that totally depends on your skills.
> > On paper the Cressington Turbo Carbon Coater 208 Carbon looks fine too,
> > I used it once and the result was ok.
> >
> > An ultimate machine would be an R&D class deposition system with the
> > e-beam gold evaporation, but the price is eye-watering.
> >
> > Best,
> > Eugene
> >
> > On Thu, Sep 14, 2023 at 12:44 PM Jasnin, Marion <jasnin at biochem.mpg.de
> > <mailto:jasnin at biochem.mpg.de <jasnin at biochem.mpg.de>>> wrote:
> >
> >     Dear all,
> >
> >     we are in the process of buying a carbon evaporator for producing
> >     additional 5-15nm support films in SPA. Our experience in making
> >     carbon films is based on older machines that are no longer
> >     available. So far, we got two quotes more on the high-end side of
> >     the instrument spectrum but considering the good quality films we
> >     were able to produce with the old machines we are wondering if such
> >     an investment is necessary for SPA:
> >
> >     Leica EM ACE600 Carbon Thread Coater
> >     * Carbon thread evaporation
> >     * Oil free UHV pump system with turbomolecular pump
> >     * Quartz crystal thickness measurement
> >     * Sample stage rotation
> >
> >     Quorum Q150V E Plus
> >     * Carbon rod evaporation
> >     * Oil free UHV pump system with turbomolecular pump
> >     * Quartz crystal thickness measurement
> >     * Sample stage rotation
> >
> >     Much cheaper options like the Leica EM ACE200 exist. We would
> >     appreciate some advice on how important expensive features like
> >     ultra high vacuum or sample stage rotation really are for SPA.
> >     Hints which instrument you are successfully using for this
> >     application would also be very helpful.
> >
> >     Thank you,
> >     Cheers,
> >     Marion
> >
> >
> >     --
> >     Marion Jasnin
> >     Principal Investigator
> >     Helmholtz Pioneer Campus
> >     Helmholtz Munich
> >     Ingolstädter Landstraße 1
> >     85764 Neuherberg near Munich
> >     Germany
> >     Phone: +49 (0)89 3187 43896
> >     marion.jasnin at helmholtz-munich.de
> >     <mailto:marion.jasnin at helmholtz-munich.de
> <marion.jasnin at helmholtz-munich.de>>
> >     <
> https://urldefense.com/v3/__https://eur05.safelinks.protection.outlook.com/?url=https*3A*2F*2Furldefense.com*2Fv3*2F__https*3A*2F*2Fwww.pioneercampus.org*2Findex.php*3Fid*3D56765__*3B!!Mih3wA!F2B2cDp2iYPLULjPeYX9Zpq415PD35oUwL6qZWVQ8tKrarfQ8W94qf1hfMSXhG15u2dRe7W4TxPvHAtwBCm2orM*24&data=05*7C01*7Cmarion.jasnin*40helmholtz-munich.de*7C534a002e53e24d95570a08dbb51bb3c1*7Ce229e4931bf240a79b8485f6c23aeed8*7C0*7C0*7C638302902821492632*7CUnknown*7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0*3D*7C3000*7C*7C*7C&sdata=DVHSXRF98STPvCE0u2c1jugSV7t7k0SHr2jF21DbvM4*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSU!!Mih3wA!FlkhjwIYlwHhlQyFkOS5SptIwbqZJH1v6r9rL8bTCVzL4RpNGxoDWXiFUXOajufMWn1m-x4XccGz1wJEWNA$ 
> <https://urldefense.com/v3/__https://www.pioneercampus.org/index.php?id=56765__;!!Mih3wA!F2B2cDp2iYPLULjPeYX9Zpq415PD35oUwL6qZWVQ8tKrarfQ8W94qf1hfMSXhG15u2dRe7W4TxPvHAtwBCm2orM$>
> >
> https://urldefense.com/v3/__https://eur05.safelinks.protection.outlook.com/?url=https*3A*2F*2Furldefense.com*2Fv3*2F__https*3A*2F*2Fwww.pioneercampus.org*2Findex.php*3Fid*3D56765__*3B!!Mih3wA!HHT2aQrJRaU9ta7uUtojcvkFaNSBPbLXcwnXgrr7-gtFX4baeWeEaCHmYWYuj-VAjhq56WjkkCm96xM2AqM7*24&data=05*7C01*7Cmarion.jasnin*40helmholtz-munich.de*7C534a002e53e24d95570a08dbb51bb3c1*7Ce229e4931bf240a79b8485f6c23aeed8*7C0*7C0*7C638302902821492632*7CUnknown*7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0*3D*7C3000*7C*7C*7C&sdata=xRfD*2FiAHF*2FNxIk*2B6XEBKcawJ475LanfjbdgQcdkqh5U*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSU!!Mih3wA!FlkhjwIYlwHhlQyFkOS5SptIwbqZJH1v6r9rL8bTCVzL4RpNGxoDWXiFUXOajufMWn1m-x4XccGz-K2bDU8$ 
> <https://urldefense.com/v3/__https://eur05.safelinks.protection.outlook.com/?url=https*3A*2F*2Furldefense.com*2Fv3*2F__https*3A*2F*2Fwww.pioneercampus.org*2Findex.php*3Fid*3D56765__*3B!!Mih3wA!HHT2aQrJRaU9ta7uUtojcvkFaNSBPbLXcwnXgrr7-gtFX4baeWeEaCHmYWYuj-VAjhq56WjkkCm96xM2AqM7*24&data=05*7C01*7Cmarion.jasnin*40helmholtz-munich.de*7C534a002e53e24d95570a08dbb51bb3c1*7Ce229e4931bf240a79b8485f6c23aeed8*7C0*7C0*7C638302902821492632*7CUnknown*7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0*3D*7C3000*7C*7C*7C&sdata=xRfD*2FiAHF*2FNxIk*2B6XEBKcawJ475LanfjbdgQcdkqh5U*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSU!!Mih3wA!FyknVE-tXG-Wr0F_Hoxh8GdT2aBgr19f8SGyJGGdxSvhQ3gqz_nfdhk6jhOAsjoAwUTwD-AsZR86HWDEdaz96luVdgQ29rgiO-3Q6Q$>
> <
> https://urldefense.com/v3/__https://eur05.safelinks.protection.outlook.com/?url=https*3A*2F*2Furldefense.com*2Fv3*2F__https*3A*2F*2Fwww.pioneercampus.org*2Findex.php*3Fid*3D56765__*3B!!Mih3wA!F2B2cDp2iYPLULjPeYX9Zpq415PD35oUwL6qZWVQ8tKrarfQ8W94qf1hfMSXhG15u2dRe7W4TxPvHAtwBCm2orM*24&data=05*7C01*7Cmarion.jasnin*40helmholtz-munich.de*7C534a002e53e24d95570a08dbb51bb3c1*7Ce229e4931bf240a79b8485f6c23aeed8*7C0*7C0*7C638302902821492632*7CUnknown*7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0*3D*7C3000*7C*7C*7C&sdata=DVHSXRF98STPvCE0u2c1jugSV7t7k0SHr2jF21DbvM4*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSU!!Mih3wA!FlkhjwIYlwHhlQyFkOS5SptIwbqZJH1v6r9rL8bTCVzL4RpNGxoDWXiFUXOajufMWn1m-x4XccGz1wJEWNA$ 
> <https://urldefense.com/v3/__https://www.pioneercampus.org/index.php?id=56765__;!!Mih3wA!F2B2cDp2iYPLULjPeYX9Zpq415PD35oUwL6qZWVQ8tKrarfQ8W94qf1hfMSXhG15u2dRe7W4TxPvHAtwBCm2orM$>
> >
> >
> >     _______________________________________________
> >     3dem mailing list
> >     3dem at ncmir.ucsd.edu <mailto:3dem at ncmir.ucsd.edu
> <3dem at ncmir.ucsd.edu>>
> >
> https://urldefense.com/v3/__https://eur05.safelinks.protection.outlook.com/?url=https*3A*2F*2Fmail.ncmir.ucsd.edu*2Fmailman*2Flistinfo*2F3dem&data=05*7C01*7Cmarion.jasnin*40helmholtz-munich.de*7C534a002e53e24d95570a08dbb51bb3c1*7Ce229e4931bf240a79b8485f6c23aeed8*7C0*7C0*7C638302902821492632*7CUnknown*7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0*3D*7C3000*7C*7C*7C&sdata=C1YsYsa4WlvndmCpZK8Oy*2FPBMYh2APsbbmUPik1vSoE*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJSUlJSUlJSU!!Mih3wA!FlkhjwIYlwHhlQyFkOS5SptIwbqZJH1v6r9rL8bTCVzL4RpNGxoDWXiFUXOajufMWn1m-x4XccGzvQNq1Rc$ 
> <https://urldefense.com/v3/__https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem__;!!JFdNOqOXpB6UZW0!udezFnTthjN7a0jmnYJX04EFvSvfudas0h_kqV_7Bsuwjo3CArCHsX7Kdo-okHSDVXRP1NINXAJuspC3nWQOucVImKSMfuU7Z6up$>
> >     <
> https://urldefense.com/v3/__https://eur05.safelinks.protection.outlook.com/?url=https*3A*2F*2Fmail.ncmir.ucsd.edu*2Fmailman*2Flistinfo*2F3dem&data=05*7C01*7Cmarion.jasnin*40helmholtz-munich.de*7C534a002e53e24d95570a08dbb51bb3c1*7Ce229e4931bf240a79b8485f6c23aeed8*7C0*7C0*7C638302902821492632*7CUnknown*7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0*3D*7C3000*7C*7C*7C&sdata=C1YsYsa4WlvndmCpZK8Oy*2FPBMYh2APsbbmUPik1vSoE*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJSUlJSUlJSU!!Mih3wA!FlkhjwIYlwHhlQyFkOS5SptIwbqZJH1v6r9rL8bTCVzL4RpNGxoDWXiFUXOajufMWn1m-x4XccGzvQNq1Rc$ 
> <https://urldefense.com/v3/__https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem__;!!JFdNOqOXpB6UZW0!udezFnTthjN7a0jmnYJX04EFvSvfudas0h_kqV_7Bsuwjo3CArCHsX7Kdo-okHSDVXRP1NINXAJuspC3nWQOucVImKSMfuU7Z6up$>
> >
> >
> >
> > _______________________________________________
> > 3dem mailing list
> > 3dem at ncmir.ucsd.edu
> >
> https://urldefense.com/v3/__https://eur05.safelinks.protection.outlook.com/?url=https*3A*2F*2Fmail.ncmir.ucsd.edu*2Fmailman*2Flistinfo*2F3dem&data=05*7C01*7Cmarion.jasnin*40helmholtz-munich.de*7C534a002e53e24d95570a08dbb51bb3c1*7Ce229e4931bf240a79b8485f6c23aeed8*7C0*7C0*7C638302902821492632*7CUnknown*7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0*3D*7C3000*7C*7C*7C&sdata=C1YsYsa4WlvndmCpZK8Oy*2FPBMYh2APsbbmUPik1vSoE*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJSUlJSUlJSU!!Mih3wA!FlkhjwIYlwHhlQyFkOS5SptIwbqZJH1v6r9rL8bTCVzL4RpNGxoDWXiFUXOajufMWn1m-x4XccGzvQNq1Rc$ 
> <https://urldefense.com/v3/__https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem__;!!JFdNOqOXpB6UZW0!udezFnTthjN7a0jmnYJX04EFvSvfudas0h_kqV_7Bsuwjo3CArCHsX7Kdo-okHSDVXRP1NINXAJuspC3nWQOucVImKSMfuU7Z6up$>
>
> --
> Artemis Kosta
> Service de microscopie, RdC BatN
>
> FR3479 Institut de Microbiologie de la Méditerranée
> 31 chemin Joseph Aiguier,
> CS 70071 - 13402 Marseille cedex 09 France
>
> ****Attention: livraisons au Bat N****
>
> akosta at imm.cnrs.fr
> 0033 491 164055
>
> https://urldefense.com/v3/__https://eur05.safelinks.protection.outlook.com/?url=https*3A*2F*2Furldefense.com*2Fv3*2F__http*3A*2F*2Fwww.imm.cnrs.fr*2Fmicroscopie*2F__*3B!!Mih3wA!HHT2aQrJRaU9ta7uUtojcvkFaNSBPbLXcwnXgrr7-gtFX4baeWeEaCHmYWYuj-VAjhq56WjkkCm96zB4Ik51*24&data=05*7C01*7Cmarion.jasnin*40helmholtz-munich.de*7C534a002e53e24d95570a08dbb51bb3c1*7Ce229e4931bf240a79b8485f6c23aeed8*7C0*7C0*7C638302902821492632*7CUnknown*7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0*3D*7C3000*7C*7C*7C&sdata=ICgxYuN4EFWB9I*2BbjiFEt*2Brha7y*2BwtqhY8LbVsqVn9I*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJQ!!Mih3wA!FlkhjwIYlwHhlQyFkOS5SptIwbqZJH1v6r9rL8bTCVzL4RpNGxoDWXiFUXOajufMWn1m-x4XccGzfJECasA$ 
> <https://urldefense.com/v3/__http://www.imm.cnrs.fr/microscopie/__;!!Mih3wA!HHT2aQrJRaU9ta7uUtojcvkFaNSBPbLXcwnXgrr7-gtFX4baeWeEaCHmYWYuj-VAjhq56WjkkCm96zB4Ik51$>
>
> https://urldefense.com/v3/__https://eur05.safelinks.protection.outlook.com/?url=https*3A*2F*2Furldefense.com*2Fv3*2F__https*3A*2F*2Firis.science-it.ch*2FLanding*2FProvider*2F2141__*3B!!Mih3wA!HHT2aQrJRaU9ta7uUtojcvkFaNSBPbLXcwnXgrr7-gtFX4baeWeEaCHmYWYuj-VAjhq56WjkkCm961lwh2CD*24&data=05*7C01*7Cmarion.jasnin*40helmholtz-munich.de*7C534a002e53e24d95570a08dbb51bb3c1*7Ce229e4931bf240a79b8485f6c23aeed8*7C0*7C0*7C638302902821492632*7CUnknown*7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0*3D*7C3000*7C*7C*7C&sdata=382pM*2F0dfD0uJBBkWvWhDf6VX1b3n5Qrdtw9fHvfP5E*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUlJSUl!!Mih3wA!FlkhjwIYlwHhlQyFkOS5SptIwbqZJH1v6r9rL8bTCVzL4RpNGxoDWXiFUXOajufMWn1m-x4XccGz9f_9HRI$ 
> <https://urldefense.com/v3/__https://iris.science-it.ch/Landing/Provider/2141__;!!Mih3wA!HHT2aQrJRaU9ta7uUtojcvkFaNSBPbLXcwnXgrr7-gtFX4baeWeEaCHmYWYuj-VAjhq56WjkkCm961lwh2CD$>
> _______________________________________________
> 3dem mailing list
> 3dem at ncmir.ucsd.edu
>
> https://urldefense.com/v3/__https://eur05.safelinks.protection.outlook.com/?url=https*3A*2F*2Fmail.ncmir.ucsd.edu*2Fmailman*2Flistinfo*2F3dem&data=05*7C01*7Cmarion.jasnin*40helmholtz-munich.de*7C534a002e53e24d95570a08dbb51bb3c1*7Ce229e4931bf240a79b8485f6c23aeed8*7C0*7C0*7C638302902821492632*7CUnknown*7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0*3D*7C3000*7C*7C*7C&sdata=C1YsYsa4WlvndmCpZK8Oy*2FPBMYh2APsbbmUPik1vSoE*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJSUlJSUlJSU!!Mih3wA!FlkhjwIYlwHhlQyFkOS5SptIwbqZJH1v6r9rL8bTCVzL4RpNGxoDWXiFUXOajufMWn1m-x4XccGzvQNq1Rc$ 
> <https://urldefense.com/v3/__https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem__;!!JFdNOqOXpB6UZW0!udezFnTthjN7a0jmnYJX04EFvSvfudas0h_kqV_7Bsuwjo3CArCHsX7Kdo-okHSDVXRP1NINXAJuspC3nWQOucVImKSMfuU7Z6up$>
>
> *Helmholtz Zentrum München* – *Deutsches Forschungszentrum für Gesundheit
> und Umwelt (GmbH)*
> Ingolstädter Landstraße 1, D-85764 Neuherberg,
> https://urldefense.com/v3/__https://www.helmholtz-munich.de__;!!Mih3wA!FlkhjwIYlwHhlQyFkOS5SptIwbqZJH1v6r9rL8bTCVzL4RpNGxoDWXiFUXOajufMWn1m-x4XccGzitJcUq4$ 
> <https://urldefense.com/v3/__https://www.helmholtz-munich.de/__;!!Mih3wA!FyknVE-tXG-Wr0F_Hoxh8GdT2aBgr19f8SGyJGGdxSvhQ3gqz_nfdhk6jhOAsjoAwUTwD-AsZR86HWDEdaz96luVdgQ29ri0w76aRA$>
> Geschäftsführung: Prof. Dr. med. Dr. h.c. Matthias Tschöp |
> Aufsichtsratsvorsitzende: MinDir’in Prof. Dr. Veronika von Messling
> Registergericht: Amtsgericht München HRB 6466 | USt-IdNr. DE 129521671
>


-- 


*Gérard Pehau Arnaudet **Institut Pasteur*

*Plateforme de Bio-Imagerie Ultrastructurale (Ultrastructural BioImaging
core)*


*Et*


*UMR 3528 Biologie structurale des processus cellulaires et maladies
infectieuses25 rue du Docteur Roux *

*75724 Paris cedex 15 *

*33 1 45 68 84 93 *

*Fax : 33 1 45 68 86 81 *

*gpehau at pasteur.fr <gpehau at pasteur.fr>*
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20230915/3c8d160e/attachment.html>


More information about the 3dem mailing list