<div dir="ltr"><div>Hello Marion</div><div>We have
Cressington Turbo Carbon Coater 208 Carbon in Pasteur: we use it only to carbon coat grids for SPA with rods. We control by eyes the thickness<br></div><div>We also have the Leica ACE 600 but we only use it to carbon coat the formvar grids for sections: in my hands the
thread never gives me a good quality of carbon.</div><div>Amicalement</div><div>Gérard<br></div><div><br></div><div><br></div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">Le jeu. 14 sept. 2023 à 15:09, Marion Jasnin <<a href="mailto:marion.jasnin@helmholtz-munich.de">marion.jasnin@helmholtz-munich.de</a>> a écrit :<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div class="msg-543318742869945906">
<div dir="ltr">
<div style="font-family:Calibri,Helvetica,sans-serif;font-size:11pt;color:rgb(0,0,0)">
Dear Rachel, Eugene and Artemis,</div>
<div style="font-family:Calibri,Helvetica,sans-serif;font-size:11pt;color:rgb(0,0,0)">
<br>
</div>
<div style="font-family:Calibri,Helvetica,sans-serif;font-size:11pt;color:rgb(0,0,0)">
Many thanks for your valuable input too.</div>
<div style="font-family:Calibri,Helvetica,sans-serif;font-size:11pt;color:rgb(0,0,0)">
<br>
</div>
<div style="font-family:Calibri,Helvetica,sans-serif;font-size:11pt;color:rgb(0,0,0)">
The choice is then between the <span style="display:inline;background-color:rgb(255,255,255)">Q150V<span> ES Plus,
<span style="display:inline;background-color:rgb(255,255,255)">
CCU-010 and the</span></span></span><span style="font-family:Calibri,Helvetica,sans-serif;font-size:11pt;color:rgb(0,0,0)"> </span><span style="font-family:Calibri,Helvetica,sans-serif;font-size:11pt;display:inline;color:rgb(0,0,0)">Cressington
Turbo Carbon Coater 208.</span></div>
<div style="font-family:Calibri,Helvetica,sans-serif;font-size:11pt;color:rgb(0,0,0)">
<span style="font-family:Calibri,Helvetica,sans-serif;font-size:11pt;display:inline;color:rgb(0,0,0)"><br>
</span></div>
<div style="font-family:Calibri,Helvetica,sans-serif;font-size:11pt;color:rgb(0,0,0)">
<span style="font-family:Calibri,Helvetica,sans-serif;display:inline;background-color:rgb(255,255,255)">Thanks again!</span></div>
<div style="font-family:Calibri,Helvetica,sans-serif;font-size:11pt;color:rgb(0,0,0)">
<span style="font-family:Calibri,Helvetica,sans-serif">Cheers,</span></div>
<div style="font-family:Calibri,Helvetica,sans-serif;font-size:11pt;color:rgb(0,0,0)">
<span style="font-family:Calibri,Helvetica,sans-serif;display:inline;background-color:rgb(255,255,255)">Marion</span></div>
<div id="m_-543318742869945906appendonsend"></div>
<div style="font-family:Calibri,Helvetica,sans-serif;font-size:11pt;color:rgb(0,0,0)">
<br>
</div>
<hr style="display:inline-block;width:98%">
<div id="m_-543318742869945906divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt;color:rgb(0,0,0)"><b>From:</b> 3dem <<a href="mailto:3dem-bounces@ncmir.ucsd.edu" target="_blank">3dem-bounces@ncmir.ucsd.edu</a>> on behalf of Artemis Kosta <<a href="mailto:akosta@imm.cnrs.fr" target="_blank">akosta@imm.cnrs.fr</a>><br>
<b>Sent:</b> 14 September 2023 14:10<br>
<b>To:</b> Eugene Pichkur <<a href="mailto:eugene.pichkur@gmail.com" target="_blank">eugene.pichkur@gmail.com</a>>; Jasnin, Marion <<a href="mailto:jasnin@biochem.mpg.de" target="_blank">jasnin@biochem.mpg.de</a>><br>
<b>Cc:</b> <a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a> <<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank">3dem@ncmir.ucsd.edu</a>><br>
<b>Subject:</b> Re: [3dem] Carbon evaporator for SPA</font>
<div> </div>
</div>
<div><font size="2"><span style="font-size:11pt">
<div>We have a Cressington Turbo Carbon Coater 208 Carbon and we are very
<br>
happy also.<br>
You can combine it with a glow discharge module also.<br>
<br>
Leica EM ACE600 didn't work nicely during the demo and I think the <br>
consumables were quite expensive comparing to the carbon rods for <br>
Cressington.<br>
<br>
Best regards<br>
Artemis Kosta<br>
<br>
Le 14/09/2023 à 14:00, Eugene Pichkur a écrit :<br>
> Long story short, high vacuum is necessary for fine grains, so a <br>
> turbo/diffusion pump is a must have.<br>
> <br>
> Leica EM ACE600 is not a reliable machine, we have several problems with <br>
> it, especially with the thickness monitor. I know several other labs <br>
> where people are also not happy with it for similar reasons.<br>
> Quorum Q150V is a simple and nice machine, I had no problems with it in <br>
> several labs and used it for thin carbon film deposition, that was <br>
> successfully used for SPA work (EMD-10655). I would go with the Q150V ES <br>
> Plus version just to be able to deposit films onto the surface of <br>
> samples for SEM. But I haven't managed to get the same quality of the <br>
> film as in Quantifoil+2nm grids (these things are very-very thin and <br>
> nice) but that totally depends on your skills.<br>
> On paper the Cressington Turbo Carbon Coater 208 Carbon looks fine too, <br>
> I used it once and the result was ok.<br>
> <br>
> An ultimate machine would be an R&D class deposition system with the <br>
> e-beam gold evaporation, but the price is eye-watering.<br>
> <br>
> Best,<br>
> Eugene<br>
> <br>
> On Thu, Sep 14, 2023 at 12:44 PM Jasnin, Marion <<a href="mailto:jasnin@biochem.mpg.de" target="_blank">jasnin@biochem.mpg.de</a> <br>
> <<a href="mailto:jasnin@biochem.mpg.de" id="m_-543318742869945906OWAe10586fd-e5dc-0329-10ef-10e774f4fbd9" target="_blank">mailto:jasnin@biochem.mpg.de</a>>> wrote:<br>
> <br>
> Dear all,<br>
> <br>
> we are in the process of buying a carbon evaporator for producing<br>
> additional 5-15nm support films in SPA. Our experience in making<br>
> carbon films is based on older machines that are no longer<br>
> available. So far, we got two quotes more on the high-end side of<br>
> the instrument spectrum but considering the good quality films we<br>
> were able to produce with the old machines we are wondering if such<br>
> an investment is necessary for SPA:<br>
> <br>
> Leica EM ACE600 Carbon Thread Coater<br>
> * Carbon thread evaporation<br>
> * Oil free UHV pump system with turbomolecular pump<br>
> * Quartz crystal thickness measurement<br>
> * Sample stage rotation<br>
> <br>
> Quorum Q150V E Plus<br>
> * Carbon rod evaporation<br>
> * Oil free UHV pump system with turbomolecular pump<br>
> * Quartz crystal thickness measurement<br>
> * Sample stage rotation<br>
> <br>
> Much cheaper options like the Leica EM ACE200 exist. We would<br>
> appreciate some advice on how important expensive features like<br>
> ultra high vacuum or sample stage rotation really are for SPA. <br>
> Hints which instrument you are successfully using for this<br>
> application would also be very helpful.<br>
> <br>
> Thank you,<br>
> Cheers,<br>
> Marion<br>
> <br>
> <br>
> --<br>
> Marion Jasnin<br>
> Principal Investigator<br>
> Helmholtz Pioneer Campus<br>
> Helmholtz Munich<br>
> Ingolstädter Landstraße 1<br>
> 85764 Neuherberg near Munich<br>
> Germany<br>
> Phone: +49 (0)89 3187 43896<br>
> <a href="mailto:marion.jasnin@helmholtz-munich.de" target="_blank">marion.jasnin@helmholtz-munich.de</a><br>
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</span></font></div>
<p><font size="-1"><b>Helmholtz Zentrum München</b> – <b>Deutsches Forschungszentrum für Gesundheit und Umwelt (GmbH)</b><br>
Ingolstädter Landstraße 1, D-85764 Neuherberg, <a href="https://urldefense.com/v3/__https://www.helmholtz-munich.de/__;!!Mih3wA!FyknVE-tXG-Wr0F_Hoxh8GdT2aBgr19f8SGyJGGdxSvhQ3gqz_nfdhk6jhOAsjoAwUTwD-AsZR86HWDEdaz96luVdgQ29ri0w76aRA$" target="_blank">
https://www.helmholtz-munich.de</a><br>
Geschäftsführung: Prof. Dr. med. Dr. h.c. Matthias Tschöp | Aufsichtsratsvorsitzende: MinDir’in Prof. Dr. Veronika von Messling<br>
Registergericht: Amtsgericht München HRB 6466 | USt-IdNr. DE 129521671 </font></p>
</div>
</div></blockquote></div><br clear="all"><br><span class="gmail_signature_prefix">-- </span><br><div dir="ltr" class="gmail_signature"><div dir="ltr"><b>Gérard Pehau Arnaudet
<br><br></b><b>Institut Pasteur</b><div><br><b><span style="color:rgb(0,0,0)">Plateforme de Bio-Imagerie Ultrastructurale (Ultrastructural BioImaging core)</span></b></div><div><b><span style="color:rgb(0,0,0)"><br></span></b></div><div><b><span style="color:rgb(0,0,0)">Et<br></span></b></div><div><div><b>UMR 3528 Biologie structurale des processus cellulaires et maladies infectieuses<br><br>25 rue du Docteur Roux
</b>
<b><br><br>75724 Paris cedex 15
</b>
<b><br><br>33 1 45 68 84 93
</b>
<b><br><br>Fax : 33 1 45 68 86 81
</b>
<b><br><br><a href="mailto:gpehau@pasteur.fr" target="_blank">gpehau@pasteur.fr</a></b>
</div></div></div></div>