[3dem] Refining polished particles

[Jay Rai]

In addition
The 4 angstroms reference map is unfiltered half maps on relion 4.

With kind regards,
Jay

________________________________
From: 3dem <3dem-bounces at ncmir.ucsd.edu> on behalf of Jay Rai <jrai at fsu.edu>
Sent: Saturday, January 8, 2022 9:29:39 PM
To: 3dem <3dem-bounces at ncmir.ucsd.edu>; 3dem at ncmir.ucsd.edu <3dem at ncmir.ucsd.edu>
Subject: [3dem] Refining polished particles

Dear all,
Can we use 4 angstroms reference map during refinement of polish particles? Also can we also do only local alignment/refinement only if the particles before polishing yield 2.5 angstroms ( with each unfiltered half maps are 3.5 angstroms).
Do you think it will be a model bias?
Any suggestions or thought are appreciated.

With kind regards,
Jay

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