[3dem] Refining polished particles

[Jay Rai]

Dear all,
Can we use 4 angstroms reference map during refinement of polish particles? Also can we also do only local alignment/refinement only if the particles before polishing yield 2.5 angstroms ( with each unfiltered half maps are 3.5 angstroms).
Do you think it will be a model bias?
Any suggestions or thought are appreciated.

With kind regards,
Jay

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