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<div dir="ltr">In addition </div>
<div dir="ltr">The 4 angstroms reference map is unfiltered half maps on relion 4.</div>
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<div style="direction:ltr">With kind regards,</div>
<div style="direction:ltr">Jay</div>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> 3dem <3dem-bounces@ncmir.ucsd.edu> on behalf of Jay Rai <jrai@fsu.edu><br>
<b>Sent:</b> Saturday, January 8, 2022 9:29:39 PM<br>
<b>To:</b> 3dem <3dem-bounces@ncmir.ucsd.edu>; 3dem@ncmir.ucsd.edu <3dem@ncmir.ucsd.edu><br>
<b>Subject:</b> [3dem] Refining polished particles</font>
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<div dir="ltr">Dear all,</div>
<div dir="ltr">Can we use 4 angstroms reference map during refinement of polish particles? Also can we also do only local alignment/refinement only if the particles before polishing yield 2.5 angstroms ( with each unfiltered half maps are 3.5 angstroms).</div>
<div dir="ltr">Do you think it will be a model bias? </div>
<div dir="ltr">Any suggestions or thought are appreciated.</div>
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<div style="direction:ltr">With kind regards,</div>
<div style="direction:ltr">Jay</div>
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