[3dem] Grid boxes for less ice contamination

Thu Feb 10 13:25:10 PST 2022

Dear Grigory

Its not difficult, but without the right ink the mark could fade over time. We use insoluble black ink during our assembly process. The grid boxes can be washed with up to 100 % ethanol to remove any writing without to worry about the mark around the opening.

If you want customized grid boxes or have more questions, you can address us directly over order at mygridbox.com

Best regards

Armin Picenoni

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Message: 2
Date: Thu, 10 Feb 2022 10:09:44 +0000
From: Grigory Sharov <sharov.grigory at gmail.com>
To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
Subject: Re: [3dem] Grid boxes for less ice contamination
Message-ID:
        <CAJZ7w0c42XnqFHcSc3qa80hNhjZ8rKrR=EeXCz+=Tb9dqhpd-g at mail.gmail.com>
Content-Type: text/plain; charset="utf-8"

Is it difficult to have the slot of the transparent lid pre-painted so one
can see which slot is open? Or do you have to paint every box with a marker?

https://urldefense.proofpoint.com/v2/url?u=https-3A__mygridbox.com_wp-2Dcontent_uploads_2022_02_Loading-5F1.png&d=DwIFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=yeOPGAhyPo5EPKsbNn1IMF-0wZ1kqyNe6L5VJLAxZJ5hoUwN23HQ1Xqn9R7FY9n7&s=BQoQJ6AgnzL8NrmrmVkdLy64mlHU8-ixLH1tfLGjz-8&e=

Best regards,
Grigory

--------------------------------------------------------------------------------
Grigory Sharov, Ph.D.

MRC Laboratory of Molecular Biology,
Francis Crick Avenue,
Cambridge Biomedical Campus,
Cambridge CB2 0QH, UK.
tel. +44 (0) 1223 267228 <+44%201223%20267228>
e-mail: gsharov at mrc-lmb.cam.ac.uk

On Thu, Feb 10, 2022 at 10:03 AM Picenoni Armin <
armin.picenoni at mol.biol.ethz.ch> wrote:

> Dear colleagues,
>
> In the past, we had several complaints about the grid boxes available on
> the market. Having to remove the lid completely to access a single grid
> brings a certain risk for all other stored grids. And needing different
> grid boxes for clipped and unclipped grids is more expensive and requires
> more organization to stock them. It also limits your storage capacity,
> especially since most can only fit four grids. Handling fib milled grids
> with pointed tweezers can damage lamellae, and although broader-tipped
> tweezers help, it?s impossible to take grids out of the boxes with them. We
> also often found the contrast poor with the currently available grid boxes.
>
> A year and a half ago, we developed grid boxes ourselves to eliminate all
> these problems. The new boxes became very popular in the institute and
> recently other universities have also asked for them. We have now created
> a little website to share them with the cryo-em community. If you?re
> interested, visit mygridbox.com.
>
> Hope it helps!
>
> Kind Regards
> Armin Picenoni
>
>
> ______________________________________
> ETH Z?rich
> Armin Picenoni
> Institute of Molecular Biology and Biophysics
> Otto-Stern-Weg 5, HPK H10
> 8093 Z?rich
> Switzerland
>
> phone: +41 (0)44 6337 596
> mail to: armin.picenoni at mol.biol.ethz.ch
> _______________________________________________
> 3dem mailing list
> 3dem at ncmir.ucsd.edu
> https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
>
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Message: 3
Date: Thu, 10 Feb 2022 06:00:00 -0800
From: Alexis Rohou <rohoua at gene.com>
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] contract research scientist position open
Message-ID:
        <CAKqCJH-XkBny7HUox8cYGM+r3Yy8JNs4tLkt1PLcchO1Y3eyZg at mail.gmail.com>
Content-Type: text/plain; charset="utf-8"

Dear colleagues,

The cryoEM and native mass spectrometry teams at Genentech (South San
Francisco, California) are looking to recruit a joint contract research
scientist (PhD level) for a 1-year term (with potential to extend) to work
on a project involving hardware development.

The project will use complementary technologies across ion mobility, mass
spectrometry, and imaging to inform protein structure. We're hoping to find
someone with experience in a cryoEM lab, who really enjoys working with
instruments and data analysis. Experience with engineering/mass
spectrometry would be a bonus, but an interest on that front would be
sufficient. There will be opportunity for publication and dissemination of
results, and significant room for independent work and leadership if
desired.

Please do not hesitate to contact me to find out more.

Cheers,
Alexis

--
*Alexis Rohou*
Principal Scientist
Genentech, Department of Structural Biology
1 DNA Way
South San Francisco, CA 94080
(cell) 781 249 9629
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Message: 4
Date: Thu, 10 Feb 2022 19:18:48 +0100
From: Anna Sartori Rupp <anna.sartori.rupp at gmail.com>
To: 3dem at ncmir.ucsd.edu
Subject: [3dem] Post-Doctoral position @Institut Pasteur, Paris:
        cryo-CLEM for Sars-CoV-2 cell interaction studies
Message-ID:
        <CAASPZDjEnNnU7Zk6M5nJJf1s4Vu7hCZp_OCWjHCm3K1aS_sQOg at mail.gmail.com>
Content-Type: text/plain; charset="utf-8"

Dear all,

A three years Post-Doctoral/Engineer position is available for a highly
motivated candidate to work on advanced *3D cryo-CLEM pipelines* to
study *Sars-CoV-2
cell interactions* at *Institut Pasteur, Paris*.

This technological project will be carried out in a stimulating
multidisciplinary environment, between research groups and imaging
facilities. The candidate will work with me at the cryo-EM facility
<https://urldefense.proofpoint.com/v2/url?u=https-3A__research.pasteur.fr_en_team_nanoimaging_&d=DwIBaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=CCl-Gxun6pwgoG5wQc4Xa7IDpoixXZL816WEz9M4Yhf_mYrfoiGtIDGXFIVyq4cn&s=mRnG1y1Iqvoy-EBO8vjlViL2ukojb6iqAMiTgs5W7d4&e= > of Institut Pasteur in
Paris and in close contact with the group of Anne Brelot in the Dynamics of
Host-Pathogen Interaction Unit directed by Jost Enninga. He/she will have
the cutting-edge optical and cryo-electron microscopy infrastructure and
expertise of Institut Pasteur at his/her disposal to integrate
super-resolution methods in the project.

The cryo-EM facility <https://urldefense.proofpoint.com/v2/url?u=https-3A__research.pasteur.fr_en_team_nanoimaging_&d=DwIBaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=CCl-Gxun6pwgoG5wQc4Xa7IDpoixXZL816WEz9M4Yhf_mYrfoiGtIDGXFIVyq4cn&s=mRnG1y1Iqvoy-EBO8vjlViL2ukojb6iqAMiTgs5W7d4&e= > of
Institut Pasteur features state-of-the-art instrumentation for single
particles and cellular studies, including a TitanKrios G3i Cryo-TEM
equipped with a bioquantum imaging filter and a K3 direct electron detector
and phase plates, two Glacios with Falcon III detector and Ceta-D camera
for MicroED analysis, an Aquilos cryo-FIB/SEM system with cryo-lift-out and
iFLM module, a Leica cryo-light Thunder Imager EM cryo-CLEM microscope.

Apply and join us on this exciting project in a unique scientific
environment in the very heart of Paris!

For more details about the job offer, please visit:

https://urldefense.proofpoint.com/v2/url?u=https-3A__research.pasteur.fr_en_job_post-2Ddoctoral-2Dfellowship-2Din-2Dcorrelative-2Dcryo-2Dlight-2Delectron-2Dmicroscopy-2Dcryo-2Dclem-2Dto-2Dstudy-2Dsars-2Dcov-2D2-2Dcell-2Dinteractions_&d=DwIBaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=CCl-Gxun6pwgoG5wQc4Xa7IDpoixXZL816WEz9M4Yhf_mYrfoiGtIDGXFIVyq4cn&s=6VP00L89tN4BVT1Ze3L1aTH60vnrpN9VkyiPN7EK8AM&e=

&

https://urldefense.proofpoint.com/v2/url?u=https-3A__research.pasteur.fr_en_member_anna-2Dsartori-2Drupp_&d=DwIBaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=CCl-Gxun6pwgoG5wQc4Xa7IDpoixXZL816WEz9M4Yhf_mYrfoiGtIDGXFIVyq4cn&s=UNJD3hG6-wkqhu9zBaopdcbnbI8l5lQ2a_e1TFfPr7M&e=

https://urldefense.proofpoint.com/v2/url?u=https-3A__research.pasteur.fr_en_member_jost-2Denninga_&d=DwIBaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=CCl-Gxun6pwgoG5wQc4Xa7IDpoixXZL816WEz9M4Yhf_mYrfoiGtIDGXFIVyq4cn&s=bXzTAi2kCZBpebX4LJuZ_I4MYqbh0qb0bDrbandAAj8&e=

https://urldefense.proofpoint.com/v2/url?u=https-3A__research.pasteur.fr_en_member_anne-2Dbrelot_&d=DwIBaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=CCl-Gxun6pwgoG5wQc4Xa7IDpoixXZL816WEz9M4Yhf_mYrfoiGtIDGXFIVyq4cn&s=uYJAoTQYuEhCpfeheUEUyGS3KFyN0xVg6EeXkx0da0s&e=

Do not hesitate to contact:

anna.sartori-rupp at pasteur.fr, anne.brelot at pasteur.fr and
jost.enninga at pasteur.fr

if you have any questions.

Best wishes

Anna

-------------------------------------------------

*Anna Sartori-Rupp*

Ultrastructural BioImaging Core facility

Institut Pasteur
28 rue du Dr Roux
75015 Paris, France

Email: anna.sartori-rupp at pasteur.fr

Tel: +33-(0)1-45.68.87.10

https://urldefense.proofpoint.com/v2/url?u=https-3A__research.pasteur.fr_en_team_ultrastructural-2Dbioimaging-2Dutechs-2Dubi_&d=DwIBaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=CCl-Gxun6pwgoG5wQc4Xa7IDpoixXZL816WEz9M4Yhf_mYrfoiGtIDGXFIVyq4cn&s=MlT7EpxCAexl96AIccgicIf9JhTbkSUL6NNJeu37TTs&e=

[image: image.png]

*https://urldefense.proofpoint.com/v2/url?u=http-3A__www.comulis.eu&d=DwIBaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=CCl-Gxun6pwgoG5wQc4Xa7IDpoixXZL816WEz9M4Yhf_mYrfoiGtIDGXFIVyq4cn&s=5eBvzcUpYa3zg4RefHNaSrkuHWHtwnFS8I9BTzPMG2A&e=  <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.comulis.eu&d=DwIBaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=CCl-Gxun6pwgoG5wQc4Xa7IDpoixXZL816WEz9M4Yhf_mYrfoiGtIDGXFIVyq4cn&s=5eBvzcUpYa3zg4RefHNaSrkuHWHtwnFS8I9BTzPMG2A&e= >*
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