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<p>Dear Grigory </p>
<p><span style="font-size: 10pt;"><br>
</span></p>
<p><span style="font-size: 10pt;">Its not difficult, but without the right ink the mark could fade over time. We use insoluble black ink during our assembly process. The grid boxes can be washed with up to 100 % ethanol to remove any writing without to worry
about the mark around the opening. </span><br>
</p>
<p><br>
</p>
<p><span>If you want customized grid boxes or have more questions, you can address us directly over order@mygridbox.com </span></p>
<p><span style="font-family: Arial, Helvetica, sans-serif, EmojiFont, "Apple Color Emoji", "Segoe UI Emoji", NotoColorEmoji, "Segoe UI Symbol", "Android Emoji", EmojiSymbols; font-size: 13.3333px;"></span></p>
<p><br>
</p>
<p>Best regards</p>
<p>Armin Picenoni</p>
<p><span style="font-size: 10pt;"></span></p>
<p><br>
</p>
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<div class="PlainText">------------------------------<br>
<br>
Message: 2<br>
Date: Thu, 10 Feb 2022 10:09:44 +0000<br>
From: Grigory Sharov <sharov.grigory@gmail.com><br>
To: "3dem@ncmir.ucsd.edu" <3dem@ncmir.ucsd.edu><br>
Subject: Re: [3dem] Grid boxes for less ice contamination<br>
Message-ID:<br>
<CAJZ7w0c42XnqFHcSc3qa80hNhjZ8rKrR=EeXCz+=Tb9dqhpd-g@mail.gmail.com><br>
Content-Type: text/plain; charset="utf-8"<br>
<br>
Is it difficult to have the slot of the transparent lid pre-painted so one<br>
can see which slot is open? Or do you have to paint every box with a marker?<br>
<br>
<a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__mygridbox.com_wp-2Dcontent_uploads_2022_02_Loading-5F1.png&d=DwIFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=yeOPGAhyPo5EPKsbNn1IMF-0wZ1kqyNe6L5VJLAxZJ5hoUwN23HQ1Xqn9R7FY9n7&s=BQoQJ6AgnzL8NrmrmVkdLy64mlHU8-ixLH1tfLGjz-8&e=" id="LPlnk758585" previewremoved="true">https://urldefense.proofpoint.com/v2/url?u=https-3A__mygridbox.com_wp-2Dcontent_uploads_2022_02_Loading-5F1.png&d=DwIFaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=yeOPGAhyPo5EPKsbNn1IMF-0wZ1kqyNe6L5VJLAxZJ5hoUwN23HQ1Xqn9R7FY9n7&s=BQoQJ6AgnzL8NrmrmVkdLy64mlHU8-ixLH1tfLGjz-8&e=</a>
<br>
<br>
Best regards,<br>
Grigory<br>
<br>
--------------------------------------------------------------------------------<br>
Grigory Sharov, Ph.D.<br>
<br>
MRC Laboratory of Molecular Biology,<br>
Francis Crick Avenue,<br>
Cambridge Biomedical Campus,<br>
Cambridge CB2 0QH, UK.<br>
tel. +44 (0) 1223 267228 <+44%201223%20267228><br>
e-mail: gsharov@mrc-lmb.cam.ac.uk<br>
<br>
<br>
On Thu, Feb 10, 2022 at 10:03 AM Picenoni Armin <<br>
armin.picenoni@mol.biol.ethz.ch> wrote:<br>
<br>
> Dear colleagues,<br>
><br>
> In the past, we had several complaints about the grid boxes available on<br>
> the market. Having to remove the lid completely to access a single grid<br>
> brings a certain risk for all other stored grids. And needing different<br>
> grid boxes for clipped and unclipped grids is more expensive and requires<br>
> more organization to stock them. It also limits your storage capacity,<br>
> especially since most can only fit four grids. Handling fib milled grids<br>
> with pointed tweezers can damage lamellae, and although broader-tipped<br>
> tweezers help, it?s impossible to take grids out of the boxes with them. We<br>
> also often found the contrast poor with the currently available grid boxes.<br>
><br>
> A year and a half ago, we developed grid boxes ourselves to eliminate all<br>
> these problems. The new boxes became very popular in the institute and<br>
> recently other universities have also asked for them. We have now created<br>
> a little website to share them with the cryo-em community. If you?re<br>
> interested, visit mygridbox.com.<br>
><br>
> Hope it helps!<br>
><br>
> Kind Regards<br>
> Armin Picenoni<br>
><br>
><br>
> ______________________________________<br>
> ETH Z?rich<br>
> Armin Picenoni<br>
> Institute of Molecular Biology and Biophysics<br>
> Otto-Stern-Weg 5, HPK H10<br>
> 8093 Z?rich<br>
> Switzerland<br>
><br>
> phone: +41 (0)44 6337 596<br>
> mail to: armin.picenoni@mol.biol.ethz.ch<br>
> _______________________________________________<br>
> 3dem mailing list<br>
> 3dem@ncmir.ucsd.edu<br>
> <a href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem" id="LPlnk132075" previewremoved="true">
https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a><br>
><br>
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------------------------------<br>
<br>
Message: 3<br>
Date: Thu, 10 Feb 2022 06:00:00 -0800<br>
From: Alexis Rohou <rohoua@gene.com><br>
To: 3dem@ncmir.ucsd.edu<br>
Subject: [3dem] contract research scientist position open<br>
Message-ID:<br>
<CAKqCJH-XkBny7HUox8cYGM+r3Yy8JNs4tLkt1PLcchO1Y3eyZg@mail.gmail.com><br>
Content-Type: text/plain; charset="utf-8"<br>
<br>
Dear colleagues,<br>
<br>
The cryoEM and native mass spectrometry teams at Genentech (South San<br>
Francisco, California) are looking to recruit a joint contract research<br>
scientist (PhD level) for a 1-year term (with potential to extend) to work<br>
on a project involving hardware development.<br>
<br>
The project will use complementary technologies across ion mobility, mass<br>
spectrometry, and imaging to inform protein structure. We're hoping to find<br>
someone with experience in a cryoEM lab, who really enjoys working with<br>
instruments and data analysis. Experience with engineering/mass<br>
spectrometry would be a bonus, but an interest on that front would be<br>
sufficient. There will be opportunity for publication and dissemination of<br>
results, and significant room for independent work and leadership if<br>
desired.<br>
<br>
Please do not hesitate to contact me to find out more.<br>
<br>
Cheers,<br>
Alexis<br>
<br>
-- <br>
*Alexis Rohou*<br>
Principal Scientist<br>
Genentech, Department of Structural Biology<br>
1 DNA Way<br>
South San Francisco, CA 94080<br>
(cell) 781 249 9629<br>
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------------------------------<br>
<br>
Message: 4<br>
Date: Thu, 10 Feb 2022 19:18:48 +0100<br>
From: Anna Sartori Rupp <anna.sartori.rupp@gmail.com><br>
To: 3dem@ncmir.ucsd.edu<br>
Subject: [3dem] Post-Doctoral position @Institut Pasteur, Paris:<br>
cryo-CLEM for Sars-CoV-2 cell interaction studies<br>
Message-ID:<br>
<CAASPZDjEnNnU7Zk6M5nJJf1s4Vu7hCZp_OCWjHCm3K1aS_sQOg@mail.gmail.com><br>
Content-Type: text/plain; charset="utf-8"<br>
<br>
Dear all,<br>
<br>
<br>
<br>
A three years Post-Doctoral/Engineer position is available for a highly<br>
motivated candidate to work on advanced *3D cryo-CLEM pipelines* to<br>
study *Sars-CoV-2<br>
cell interactions* at *Institut Pasteur, Paris*.<br>
<br>
<br>
<br>
This technological project will be carried out in a stimulating<br>
multidisciplinary environment, between research groups and imaging<br>
facilities. The candidate will work with me at the cryo-EM facility<br>
<<a href=""></a>https://urldefense.proofpoint.com/v2/url?u=https-3A__research.pasteur.fr_en_team_nanoimaging_&d=DwIBaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=CCl-Gxun6pwgoG5wQc4Xa7IDpoixXZL816WEz9M4Yhf_mYrfoiGtIDGXFIVyq4cn&s=mRnG1y1Iqvoy-EBO8vjlViL2ukojb6iqAMiTgs5W7d4&e=
> of Institut Pasteur in<br>
Paris and in close contact with the group of Anne Brelot in the Dynamics of<br>
Host-Pathogen Interaction Unit directed by Jost Enninga. He/she will have<br>
the cutting-edge optical and cryo-electron microscopy infrastructure and<br>
expertise of Institut Pasteur at his/her disposal to integrate<br>
super-resolution methods in the project.<br>
<br>
<br>
<br>
The cryo-EM facility <<a href=""></a>https://urldefense.proofpoint.com/v2/url?u=https-3A__research.pasteur.fr_en_team_nanoimaging_&d=DwIBaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=CCl-Gxun6pwgoG5wQc4Xa7IDpoixXZL816WEz9M4Yhf_mYrfoiGtIDGXFIVyq4cn&s=mRnG1y1Iqvoy-EBO8vjlViL2ukojb6iqAMiTgs5W7d4&e=
> of<br>
Institut Pasteur features state-of-the-art instrumentation for single<br>
particles and cellular studies, including a TitanKrios G3i Cryo-TEM<br>
equipped with a bioquantum imaging filter and a K3 direct electron detector<br>
and phase plates, two Glacios with Falcon III detector and Ceta-D camera<br>
for MicroED analysis, an Aquilos cryo-FIB/SEM system with cryo-lift-out and<br>
iFLM module, a Leica cryo-light Thunder Imager EM cryo-CLEM microscope.<br>
<br>
<br>
<br>
Apply and join us on this exciting project in a unique scientific<br>
environment in the very heart of Paris!<br>
<br>
<br>
<br>
For more details about the job offer, please visit:<br>
<br>
<a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__research.pasteur.fr_en_job_post-2Ddoctoral-2Dfellowship-2Din-2Dcorrelative-2Dcryo-2Dlight-2Delectron-2Dmicroscopy-2Dcryo-2Dclem-2Dto-2Dstudy-2Dsars-2Dcov-2D2-2Dcell-2Dinteractions_&d=DwIBaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=CCl-Gxun6pwgoG5wQc4Xa7IDpoixXZL816WEz9M4Yhf_mYrfoiGtIDGXFIVyq4cn&s=6VP00L89tN4BVT1Ze3L1aTH60vnrpN9VkyiPN7EK8AM&e=">https://urldefense.proofpoint.com/v2/url?u=https-3A__research.pasteur.fr_en_job_post-2Ddoctoral-2Dfellowship-2Din-2Dcorrelative-2Dcryo-2Dlight-2Delectron-2Dmicroscopy-2Dcryo-2Dclem-2Dto-2Dstudy-2Dsars-2Dcov-2D2-2Dcell-2Dinteractions_&d=DwIBaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=CCl-Gxun6pwgoG5wQc4Xa7IDpoixXZL816WEz9M4Yhf_mYrfoiGtIDGXFIVyq4cn&s=6VP00L89tN4BVT1Ze3L1aTH60vnrpN9VkyiPN7EK8AM&e=</a>
<br>
<br>
&<br>
<br>
<a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__research.pasteur.fr_en_member_anna-2Dsartori-2Drupp_&d=DwIBaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=CCl-Gxun6pwgoG5wQc4Xa7IDpoixXZL816WEz9M4Yhf_mYrfoiGtIDGXFIVyq4cn&s=UNJD3hG6-wkqhu9zBaopdcbnbI8l5lQ2a_e1TFfPr7M&e=">https://urldefense.proofpoint.com/v2/url?u=https-3A__research.pasteur.fr_en_member_anna-2Dsartori-2Drupp_&d=DwIBaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=CCl-Gxun6pwgoG5wQc4Xa7IDpoixXZL816WEz9M4Yhf_mYrfoiGtIDGXFIVyq4cn&s=UNJD3hG6-wkqhu9zBaopdcbnbI8l5lQ2a_e1TFfPr7M&e=</a>
<br>
<br>
<a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__research.pasteur.fr_en_member_jost-2Denninga_&d=DwIBaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=CCl-Gxun6pwgoG5wQc4Xa7IDpoixXZL816WEz9M4Yhf_mYrfoiGtIDGXFIVyq4cn&s=bXzTAi2kCZBpebX4LJuZ_I4MYqbh0qb0bDrbandAAj8&e=">https://urldefense.proofpoint.com/v2/url?u=https-3A__research.pasteur.fr_en_member_jost-2Denninga_&d=DwIBaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=CCl-Gxun6pwgoG5wQc4Xa7IDpoixXZL816WEz9M4Yhf_mYrfoiGtIDGXFIVyq4cn&s=bXzTAi2kCZBpebX4LJuZ_I4MYqbh0qb0bDrbandAAj8&e=</a>
<br>
<br>
<a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__research.pasteur.fr_en_member_anne-2Dbrelot_&d=DwIBaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=CCl-Gxun6pwgoG5wQc4Xa7IDpoixXZL816WEz9M4Yhf_mYrfoiGtIDGXFIVyq4cn&s=uYJAoTQYuEhCpfeheUEUyGS3KFyN0xVg6EeXkx0da0s&e=">https://urldefense.proofpoint.com/v2/url?u=https-3A__research.pasteur.fr_en_member_anne-2Dbrelot_&d=DwIBaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=CCl-Gxun6pwgoG5wQc4Xa7IDpoixXZL816WEz9M4Yhf_mYrfoiGtIDGXFIVyq4cn&s=uYJAoTQYuEhCpfeheUEUyGS3KFyN0xVg6EeXkx0da0s&e=</a>
<br>
<br>
<br>
<br>
Do not hesitate to contact:<br>
<br>
anna.sartori-rupp@pasteur.fr, anne.brelot@pasteur.fr and<br>
jost.enninga@pasteur.fr<br>
<br>
if you have any questions.<br>
<br>
<br>
<br>
Best wishes<br>
<br>
<br>
<br>
Anna<br>
<br>
<br>
<br>
-------------------------------------------------<br>
<br>
*Anna Sartori-Rupp*<br>
<br>
Ultrastructural BioImaging Core facility<br>
<br>
Institut Pasteur<br>
28 rue du Dr Roux<br>
75015 Paris, France<br>
<br>
Email: anna.sartori-rupp@pasteur.fr<br>
<br>
Tel: +33-(0)1-45.68.87.10<br>
<br>
<a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__research.pasteur.fr_en_team_ultrastructural-2Dbioimaging-2Dutechs-2Dubi_&d=DwIBaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=CCl-Gxun6pwgoG5wQc4Xa7IDpoixXZL816WEz9M4Yhf_mYrfoiGtIDGXFIVyq4cn&s=MlT7EpxCAexl96AIccgicIf9JhTbkSUL6NNJeu37TTs&e=">https://urldefense.proofpoint.com/v2/url?u=https-3A__research.pasteur.fr_en_team_ultrastructural-2Dbioimaging-2Dutechs-2Dubi_&d=DwIBaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=CCl-Gxun6pwgoG5wQc4Xa7IDpoixXZL816WEz9M4Yhf_mYrfoiGtIDGXFIVyq4cn&s=MlT7EpxCAexl96AIccgicIf9JhTbkSUL6NNJeu37TTs&e=</a>
<br>
<br>
<br>
[image: image.png]<br>
<br>
*https://urldefense.proofpoint.com/v2/url?u=http-3A__www.comulis.eu&d=DwIBaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=CCl-Gxun6pwgoG5wQc4Xa7IDpoixXZL816WEz9M4Yhf_mYrfoiGtIDGXFIVyq4cn&s=5eBvzcUpYa3zg4RefHNaSrkuHWHtwnFS8I9BTzPMG2A&e=
<<a href=""></a>https://urldefense.proofpoint.com/v2/url?u=http-3A__www.comulis.eu&d=DwIBaQ&c=-35OiAkTchMrZOngvJPOeA&r=L7-zyQ-04fFCMRqzLIOnx7H0exGZHwIQe_wMPuY600I&m=CCl-Gxun6pwgoG5wQc4Xa7IDpoixXZL816WEz9M4Yhf_mYrfoiGtIDGXFIVyq4cn&s=5eBvzcUpYa3zg4RefHNaSrkuHWHtwnFS8I9BTzPMG2A&e=
>*<br>
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