[3dem] 3dem Digest, Vol 155, Issue 38

[Anchi Cheng]

Hi, Krishna,

As long as you are not getting a modern TFS camera that is meant to be integrated
into the microscope interface, cameras comes with its own PC, and their most import
interaction with the scope, the shutter control, is done through hardware.  Therefore, you
should have no problem with XP.  You just want to make sure XP computer is well shielded
from internet traffic.

As far as automation, you should check if the scope has TEM Scripting Access.  That is
required by all automation software.

Best,

Anchi

> On Jul 30, 2020, at 12:03 PM, 3dem-request at ncmir.ucsd.edu wrote:
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> Today's Topics:
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>   1. Re: Tecnai T12 Camera config (Harris, Audray (NIH/NIAID) [E])
> 
> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Thu, 30 Jul 2020 18:57:46 +0000
> From: "Harris, Audray (NIH/NIAID) [E]" <harrisau at mail.nih.gov>
> To: KrishnaM <vattipalli at gmail.com>, "3dem at ncmir.ucsd.edu"
> 	<3dem at ncmir.ucsd.edu>
> Subject: Re: [3dem] Tecnai T12 Camera config
> Message-ID: <F2F353D5-FCBC-44BD-AE23-BDEE10DBFF3F at nih.gov>
> Content-Type: text/plain; charset="utf-8"
> 
> Dear Krishna
> 
> We use an old FEI Tecnai T12 with a Gatan OneView for negative-staining and preliminary sample screening.
> In fact, we published a protocol paper on  getting negative-stain 3D reconstructions for T12.
> See attached protocol. Hope it helps.
> 
> 
> 
> Gallagher, J. R., Kim, A. J., Gulati, N. M., & Harris, A. K. (2019).
> 
> Negative-stain transmission electron microscopy of molecular
> 
> complexes for image analysis by 2D class averaging. Current
> 
> Protocols in Microbiology, 54, e90. doi: 10.1002/cpmc.90
> 
> Audray Harris Ph.D.
> National Institutes of Health
> harrsiau at mail.nih.gov<mailto:harrsiau at mail.nih.gov>
> 
> 
> From: KrishnaM <vattipalli at gmail.com>
> Date: Thursday, July 30, 2020 at 2:23 PM
> To: "3dem at ncmir.ucsd.edu" <3dem at ncmir.ucsd.edu>
> Subject: [3dem] Tecnai T12 Camera config
> 
> Dear 3dem team,
> 
> We have a FEI Tecnai T12 Cryo-TEM with a eagle 2k ? 2k camera. We are looking to upgrade the bottom mounted camera for it.
> 
> Final goal is to acquire minimum of 4k ? 4k images. Also required are automation in acquisition (like leginon), drift correction, ability to annotate, and having Audit trial traceability will be ultimate!
> 
> Our existing PC config is Windows XP (Stone aged!!). Wondering if this will still work with the upgradation of the camera. Any thoughts / suggestions will be appreciated.
> 
> Best Regards
> Krishna
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