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<p class="MsoNormal">Dear Krishna<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">We use an old FEI Tecnai T12 with a Gatan OneView for negative-staining and preliminary sample screening.<o:p></o:p></p>
<p class="MsoNormal">In fact, we published a protocol paper on getting negative-stain 3D reconstructions for T12.<o:p></o:p></p>
<p class="MsoNormal">See attached protocol. Hope it helps.<o:p></o:p></p>
<p class="p1"><o:p> </o:p></p>
<p class="p1">Gallagher, J. R., Kim, A. J., Gulati, N. M., & Harris, A. K. (2019).<o:p></o:p></p>
<p class="p1">Negative-stain transmission electron microscopy of molecular<o:p></o:p></p>
<p class="p1">complexes for image analysis by 2D class averaging. Current<o:p></o:p></p>
<p class="p1">Protocols in Microbiology, 54, e90. doi: 10.1002/cpmc.90<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Audray Harris Ph.D.<o:p></o:p></p>
<p class="MsoNormal">National Institutes of Health<o:p></o:p></p>
<p class="MsoNormal"><a href="mailto:harrsiau@mail.nih.gov">harrsiau@mail.nih.gov</a><o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><b><span style="font-size:12.0pt;color:black">From: </span></b><span style="font-size:12.0pt;color:black">KrishnaM <vattipalli@gmail.com><br>
<b>Date: </b>Thursday, July 30, 2020 at 2:23 PM<br>
<b>To: </b>"3dem@ncmir.ucsd.edu" <3dem@ncmir.ucsd.edu><br>
<b>Subject: </b>[3dem] Tecnai T12 Camera config<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Dear 3dem team,<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">We have a FEI Tecnai T12 Cryo-TEM with a eagle 2k × 2k camera. We are looking to upgrade the bottom mounted camera for it. <o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Final goal is to acquire minimum of 4k × 4k images. Also required are automation in acquisition (like leginon), drift correction, ability to annotate, and having Audit trial traceability will be ultimate!<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Our existing PC config is Windows XP (Stone aged!!). Wondering if this will still work with the upgradation of the camera. Any thoughts / suggestions will be appreciated.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Best Regards<o:p></o:p></p>
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<p class="MsoNormal">Krishna<o:p></o:p></p>
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