[3dem] [ccpem] Which resolution?

[Daniel Asarnow]

Pawel,
I think we all want to know where the atoms go. Most people - not just
electron microscopists or structural biologists - think "resolution" refers
to a characteristic distance at which two points can be distinguished from
one, or within which one point can be confidently placed. So far, there has
not been a widely adopted method for determining such distances for cryo-EM
maps, which is why we like to argue about it. After all, we care a lot
about where the atoms go! Pavel's methods in Phenix, or other methods based
on ensemble modeling are a start in this direction.

Best,
-da

On Wed, Feb 12, 2020 at 6:00 PM Penczek, Pawel A <
Pawel.A.Penczek at uth.tmc.edu> wrote:

> Dear Daniel,
>
> most definitely we do not want an Abbe criterion. It is characteristic of
> imaging device, does not account for alignment procedures, SNR, and
> finally, as Marin already pointed out, simple filtration would change the
> outcome. So no, not everybody wants it.
>
> Regards,
> Pawel Penczek
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