[3dem] Filter paper cutter/puncher for Vitrobot

[Rebecca Thompson]

Hi Paolo,

Our workshop has fashioned similar stamps to create filter paper for both the Vitrobot and Leica EM GP (as on attached image). Again both start with 55mm round paper and punch a hole of the correct size for the instrument. We have been using these for many years and seem to do a good job in our hands (as long as you only do a few bits of paper at a time), although I’m sure the cutting blade is not as sharp as a lovely sub-angstrom one!

I’d be happy to connect you (or anyone else interested) with our workshop if you would like one making.

Best wishes,
Becky

[cid:00170E79-68FB-4AA9-9ED0-26E69EF4DE46 at wireless.leeds.ac.uk]
________________________________________________
Rebecca Thompson
Senior cryo-Electron Microscopy Support Scientist/Facility manager
Astbury Biostructure Laboratory
University of Leeds

Email r.f.thompson at leeds.ac.uk<mailto:r.f.thompson at leeds.ac.uk>
Phone 0113 3433384/3438957 (Office/Titan Krios control room)
Mobile 07816179813
Location Roger Stevens 5.40
Linkedin   https://uk.linkedin.com/in/1rebeccathompson
Twitter  Bex_16

On 10 Feb 2020, at 16:07, SubAngstrom <info at subangstrom.com<mailto:info at subangstrom.com>> wrote:

Thank you Melissa.

Just to add, the cutting edges are tempered; thus are extremely sharp and will not lose their cutting edge.

-SA Team

On Mon, Feb 10, 2020 at 11:01 AM Chambers, Melissa G <melissa.g.chambers at vanderbilt.edu<mailto:melissa.g.chambers at vanderbilt.edu>> wrote:
We have Subangstrom’s punch.  You do have to start with 55mm round filter paper and this punches the hole in the center.

https://subangstrom.com/Accessories/Hole-Punch55mm-20mmID

In the past, we had an on campus machine shop make one but it wasn’t sharp enough to cut cleanly or easily.


Melissa

-----
Melissa G. Chambers

Director, Vanderbilt Cryo-EM Facility
Center for Structural Biology

Vanderbilt University
RR1207 MCN
1161 21st Ave S
Nashville TN 37232

615-322-4671
www.vanderbilt.edu/csb/facilities/cryo-electron-microscopy<http://www.vanderbilt.edu/csb/facilities/cryo-electron-microscopy>


From: 3dem <3dem-bounces at ncmir.ucsd.edu<mailto:3dem-bounces at ncmir.ucsd.edu>> on behalf of Paolo Swuec <paolo.swuec at gmail.com<mailto:paolo.swuec at gmail.com>>
Date: Monday, February 10, 2020 at 9:53 AM
To: "3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu>" <3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu>>
Subject: [3dem] Filter paper cutter/puncher for Vitrobot

Dear list,

We've been using commercially available filter paper for our Vitrobot. Given the cost of this consumable (5-10x the cost of Whatman filter paper 595), I am wondering whether anyone can recommend a paper cutter/puncher that can make some from filter paper sheet. I seem to remember the Gatan CP3 comes with one (if I'm not wrong, long time no see)...

Any advice?

Best,

Paolo

------------------------------------------------
Paolo Swuec, PhD
Cryo-Electron Microscopy Lab ❆ Facility Manager
Dep. BioSciences
University of Milano
Via Celoria 26 - 20133 (Italy)
Tel. +39 02 503 147 78

Follow on twitter @PS_CryoEM

_______________________________________________
3dem mailing list
3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu>
https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
_______________________________________________
3dem mailing list
3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu>
https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem

-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20200210/41b695e6/attachment-0001.html>
-------------- next part --------------
A non-text attachment was scrubbed...
Name: IMG_20200210_162431.jpg
Type: image/jpeg
Size: 3047486 bytes
Desc: IMG_20200210_162431.jpg
URL: <http://mail.ncmir.ucsd.edu/pipermail/3dem/attachments/20200210/41b695e6/attachment-0001.jpg>