<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
</head>
<body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">
Hi Paolo,
<div class=""><br class="">
</div>
<div class="">Our workshop has fashioned similar stamps to create filter paper for both the Vitrobot and Leica EM GP (as on attached image). Again both start with 55mm round paper and punch a hole of the correct size for the instrument. We have been using these
for many years and seem to do a good job in our hands (as long as you only do a few bits of paper at a time), although I’m sure the cutting blade is not as sharp as a lovely sub-angstrom one! </div>
<div class=""><br class="">
</div>
<div class="">I’d be happy to connect you (or anyone else interested) with our workshop if you would like one making. </div>
<div class=""><br class="">
</div>
<div class="">Best wishes,</div>
<div class="">Becky</div>
<div class=""><br class="">
</div>
<div class=""><img apple-inline="yes" id="11056338-C877-4AD7-9DA5-CA644A7E8B27" src="cid:00170E79-68FB-4AA9-9ED0-26E69EF4DE46@wireless.leeds.ac.uk" class=""></div>
<div class="">
<div class="">
<div style="color: rgb(0, 0, 0); letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">
<div style="color: rgb(0, 0, 0); letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">
<div style="color: rgb(0, 0, 0); letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">
<div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">
<div style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px;">
________________________________________________</div>
<div style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px;">
Rebecca Thompson<br class="">
Senior cryo-Electron Microscopy Support Scientist/Facility manager <br class="">
Astbury Biostructure Laboratory<br class="">
University of Leeds<br class="">
<br class="">
Email<span class="Apple-tab-span" style="white-space: pre;"> </span><a href="mailto:r.f.thompson@leeds.ac.uk" class="">r.f.thompson@leeds.ac.uk</a><br class="">
Phone<span class="Apple-tab-span" style="white-space: pre;"> </span>0113 3433384/3438957 (Office/Titan Krios control room)</div>
<div style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px;">
Mobile<span class="Apple-tab-span" style="white-space: pre;"> </span>07816179813<br class="">
Location<span class="Apple-tab-span" style="white-space: pre;"> </span>Roger Stevens 5.40<br class="">
Linkedin<span class="Apple-tab-span" style="white-space: pre;"> </span> <span class="Apple-tab-span" style="white-space: pre;">
</span><a href="https://uk.linkedin.com/in/1rebeccathompson" class="">https://uk.linkedin.com/in/1rebeccathompson</a><br class="">
Twitter<span class="Apple-tab-span" style="white-space: pre;"> </span> Bex_16</div>
</div>
</div>
</div>
</div>
</div>
<br class="">
<div>
<blockquote type="cite" class="">
<div class="">On 10 Feb 2020, at 16:07, SubAngstrom <<a href="mailto:info@subangstrom.com" class="">info@subangstrom.com</a>> wrote:</div>
<br class="Apple-interchange-newline">
<div class="">
<div dir="ltr" class="">Thank you Melissa.
<div class=""><br class="">
</div>
<div class="">Just to add, the cutting edges are tempered; thus are extremely sharp and will not lose their cutting edge.</div>
<div class=""><br class="">
</div>
<div class="">-SA Team</div>
</div>
<br class="">
<div class="gmail_quote">
<div dir="ltr" class="gmail_attr">On Mon, Feb 10, 2020 at 11:01 AM Chambers, Melissa G <<a href="mailto:melissa.g.chambers@vanderbilt.edu" class="">melissa.g.chambers@vanderbilt.edu</a>> wrote:<br class="">
</div>
<blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">
<div lang="EN-US" class="">
<div class="gmail-m_6280955704290789284WordSection1">
<p class="MsoNormal">We have Subangstrom’s punch. You do have to start with 55mm round filter paper and this punches the hole in the center.<u class=""></u><u class=""></u></p>
<p class="MsoNormal"><u class=""></u> <u class=""></u></p>
<p class="MsoNormal"><a href="https://subangstrom.com/Accessories/Hole-Punch55mm-20mmID" target="_blank" class="">https://subangstrom.com/Accessories/Hole-Punch55mm-20mmID</a><u class=""></u><u class=""></u></p>
<p class="MsoNormal"><u class=""></u> <u class=""></u></p>
<p class="MsoNormal">In the past, we had an on campus machine shop make one but it wasn’t sharp enough to cut cleanly or easily.<u class=""></u><u class=""></u></p>
<p class="MsoNormal"><u class=""></u> <u class=""></u></p>
<p class="MsoNormal"><u class=""></u> <u class=""></u></p>
<p class="MsoNormal">Melissa<u class=""></u><u class=""></u></p>
<p class="MsoNormal"><u class=""></u> <u class=""></u></p>
<div class="">
<p class="MsoNormal"><span style="font-size:10pt;color:rgb(59,56,56)" class="">-----</span><span style="" class=""><u class=""></u><u class=""></u></span></p>
<p class="MsoNormal"><span style="font-size:10pt;font-family:Verdana,sans-serif;color:rgb(59,56,56)" class="">Melissa G. Chambers</span><span style="font-size: 10pt; font-family: Verdana, sans-serif;" class=""><u class=""></u><u class=""></u></span></p>
<p class="MsoNormal"><span style="font-size:10pt;font-family:Verdana,sans-serif;color:rgb(59,56,56)" class=""> </span><span style="font-size: 10pt; font-family: Verdana, sans-serif;" class=""><u class=""></u><u class=""></u></span></p>
<p class="MsoNormal"><span style="font-size:10pt;font-family:Verdana,sans-serif;color:rgb(59,56,56)" class="">Director, Vanderbilt Cryo-EM Facility</span><span style="font-size: 10pt; font-family: Verdana, sans-serif;" class=""><u class=""></u><u class=""></u></span></p>
<p class="MsoNormal"><span style="font-size:10pt;font-family:Verdana,sans-serif;color:rgb(59,56,56)" class="">Center for Structural Biology<u class=""></u><u class=""></u></span></p>
<p class="MsoNormal"><span style="font-size:10pt;font-family:Verdana,sans-serif;color:rgb(59,56,56)" class=""><u class=""></u> <u class=""></u></span></p>
<p class="MsoNormal"><span style="font-size:10pt;font-family:Verdana,sans-serif;color:rgb(59,56,56)" class="">Vanderbilt University<u class=""></u><u class=""></u></span></p>
<p class="MsoNormal"><span style="font-size:10pt;font-family:Verdana,sans-serif;color:rgb(59,56,56)" class="">RR1207 MCN<u class=""></u><u class=""></u></span></p>
<p class="MsoNormal"><span style="font-size:10pt;font-family:Verdana,sans-serif;color:rgb(59,56,56)" class="">1161 21<sup class="">st</sup> Ave S<u class=""></u><u class=""></u></span></p>
<p class="MsoNormal"><span style="font-size:10pt;font-family:Verdana,sans-serif;color:rgb(59,56,56)" class="">Nashville TN 37232<u class=""></u><u class=""></u></span></p>
<p class="MsoNormal"><span style="font-size:10pt;font-family:Verdana,sans-serif;color:rgb(59,56,56)" class=""><u class=""></u> <u class=""></u></span></p>
<p class="MsoNormal"><span style="font-size:10pt;font-family:Verdana,sans-serif;color:rgb(59,56,56)" class="">615-322-4671<u class=""></u><u class=""></u></span></p>
<p class="MsoNormal"><span style="font-size:10pt;font-family:Verdana,sans-serif" class=""><a href="http://www.vanderbilt.edu/csb/facilities/cryo-electron-microscopy" target="_blank" class="">www.vanderbilt.edu/csb/facilities/cryo-electron-microscopy</a><u class=""></u><u class=""></u></span></p>
</div>
<p class="MsoNormal"><u class=""></u> <u class=""></u></p>
<p class="MsoNormal"><u class=""></u> <u class=""></u></p>
<div style="border-right:none;border-bottom:none;border-left:none;border-top:1pt solid rgb(181,196,223);padding:3pt 0in 0in" class="">
<p class="MsoNormal"><b class=""><span style="font-size: 12pt;" class="">From: </span>
</b><span style="font-size: 12pt;" class="">3dem <<a href="mailto:3dem-bounces@ncmir.ucsd.edu" target="_blank" class="">3dem-bounces@ncmir.ucsd.edu</a>> on behalf of Paolo Swuec <<a href="mailto:paolo.swuec@gmail.com" target="_blank" class="">paolo.swuec@gmail.com</a>><br class="">
<b class="">Date: </b>Monday, February 10, 2020 at 9:53 AM<br class="">
<b class="">To: </b>"<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank" class="">3dem@ncmir.ucsd.edu</a>" <<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank" class="">3dem@ncmir.ucsd.edu</a>><br class="">
<b class="">Subject: </b>[3dem] Filter paper cutter/puncher for Vitrobot<u class=""></u><u class=""></u></span></p>
</div>
<div class="">
<p class="MsoNormal"><u class=""></u> <u class=""></u></p>
</div>
<p class="MsoNormal">Dear list, <u class=""></u><u class=""></u></p>
<div class="">
<p class="MsoNormal"><u class=""></u> <u class=""></u></p>
</div>
<div class="">
<p class="MsoNormal">We've been using commercially available filter paper for our Vitrobot. Given the cost of this consumable (5-10x the cost of Whatman filter paper 595), I am wondering whether anyone can recommend a paper cutter/puncher that can make some
from filter paper sheet. I seem to remember the Gatan CP3 comes with one (if I'm not wrong, long time no see)...<u class=""></u><u class=""></u></p>
</div>
<div class="">
<p class="MsoNormal"><u class=""></u> <u class=""></u></p>
</div>
<div class="">
<p class="MsoNormal">Any advice?<u class=""></u><u class=""></u></p>
</div>
<div class="">
<p class="MsoNormal"><u class=""></u> <u class=""></u></p>
</div>
<div class="">
<p class="MsoNormal">Best,<u class=""></u><u class=""></u></p>
</div>
<div class="">
<p class="MsoNormal"><u class=""></u> <u class=""></u></p>
</div>
<p class="MsoNormal">Paolo <u class=""></u><u class=""></u></p>
<div class="">
<p class="MsoNormal"><u class=""></u> <u class=""></u></p>
</div>
<div class="">
<p class="MsoNormal"><span style="font-family:Arial,sans-serif;color:rgb(96,96,96)" class="">------------------------------------------------</span><u class=""></u><u class=""></u></p>
<div class="">
<div class="">
<div class="">
<p class="MsoNormal"><span style="font-family:Arial,sans-serif;color:rgb(96,96,96)" class="">Paolo Swuec, PhD<u class=""></u><u class=""></u></span></p>
</div>
<div class="">
<p class="MsoNormal"><span style="font-family:Arial,sans-serif;color:rgb(96,96,96)" class="">Cryo-Electron Microscopy Lab
</span><span style="font-family:"Segoe UI Symbol",sans-serif;color:rgb(96,96,96)" class="">❆</span><span style="font-family:Arial,sans-serif;color:rgb(96,96,96)" class=""> Facility Manager<u class=""></u><u class=""></u></span></p>
</div>
<div class="">
<p class="MsoNormal"><span style="font-family:Arial,sans-serif;color:rgb(96,96,96)" class="">Dep. BioSciences<u class=""></u><u class=""></u></span></p>
</div>
<div class="">
<p class="MsoNormal"><span style="font-family:Arial,sans-serif;color:rgb(96,96,96)" class="">University of Milano<u class=""></u><u class=""></u></span></p>
</div>
<div class="">
<p class="MsoNormal"><span style="font-family:Arial,sans-serif;color:rgb(96,96,96)" class="">Via Celoria 26 - 20133 (Italy)<u class=""></u><u class=""></u></span></p>
</div>
<div class="">
<p class="MsoNormal"><span style="font-family:Arial,sans-serif;color:rgb(96,96,96)" class="">Tel. +39 02 503 147 78<u class=""></u><u class=""></u></span></p>
</div>
<div class="">
<p class="MsoNormal"><span style="font-family:Arial,sans-serif;color:rgb(96,96,96)" class=""><u class=""></u> <u class=""></u></span></p>
</div>
<div class="">
<p class="MsoNormal"><span style="font-family:Arial,sans-serif;color:rgb(96,96,96)" class="">Follow on twitter @PS_CryoEM<u class=""></u><u class=""></u></span></p>
</div>
</div>
</div>
<p class="MsoNormal"><u class=""></u> <u class=""></u></p>
</div>
</div>
</div>
_______________________________________________<br class="">
3dem mailing list<br class="">
<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank" class="">3dem@ncmir.ucsd.edu</a><br class="">
<a href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem" rel="noreferrer" target="_blank" class="">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a><br class="">
</blockquote>
</div>
_______________________________________________<br class="">
3dem mailing list<br class="">
<a href="mailto:3dem@ncmir.ucsd.edu" class="">3dem@ncmir.ucsd.edu</a><br class="">
https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem<br class="">
</div>
</blockquote>
</div>
<br class="">
</div>
</body>
</html>