[3dem] side entry holder for autogrids?

[Haifeng He]

Actually in my case it's the first case, the one with the thread, but not
the two feet. I just checked under an optical microscope and see the ring
and spring that holds the ring all look ok. However, I do see a problem at
least in my case: the autogrids slide freely under the clip ring---so
expect some drift issue. I can think of two solutions:
1. a washer at the right size. This will make the autogrid+washer bigger
and bigger, but should be ok.
2. file the clip ring precisely so that the clip ring can go in the
"normal" way (you can't push it in the normal way without making it
shorter) and in the meantime hold the autogrid firmly.

Haifeng

On Wed, Jun 26, 2019 at 12:07 PM Sharon G Wolf <Sharon.Wolf at weizmann.ac.il>
wrote:

> David
> In our case it is the second type with the “feet” tool. That corresponds
> to the “626-70” holder. In the first case ( the tool with the threaded end)
> it’s the 626-60 holder. We have one of the 626-60 holders too,  but did
> this successful  test with the 626-70 holder only.
> Sharon
>
> Sent from my iPhone
>
> On 26 Jun 2019, at 19:01, David Michael Belnap <David.Belnap at utah.edu>
> wrote:
>
> Michael and Haifeng,
>
> What type of the 626 holder are you using?
>
> -One type has phosphor bronze clip ring that appears a bit slanted on the
> side that faces the grid.  The inside of the clip ring has threads.  The
> tool used to put them in place and remove them is a rod with a blunt end
> and a threaded end.
>
> -The other type uses Be clip rings that are more flat.  The clip rings
> resemble the Krios clip rings in that the Be ring has a raised portion on
> the outer diameter.  The tool used to insert and remove them has small
> “feet” that the user spreads out to about 180 degrees to hook against the
> clip ring to manipulate it.
>
> Thanks.
>
> David
>
>
> On Jun 25, 2019, at 3:18 PM, Haifeng He <haifenghe at gmail.com> wrote:
>
> I just tried what you said in the loading station only and it worked! The
> shutter also closes nicely. Great idea! Will still need to see if the drift
> rate is affected at Cryo temperature.
>
> Haifeng
>
> On Tue, Jun 25, 2019 at 3:49 PM Michael Elbaum <
> michael.elbaum at weizmann.ac.il> wrote:
>
>> Regarding my earlier post about a side entry holder for autogrids, here's
>> a simple hack. The autogrid cassette sits comfortably in the 626-70 holder
>> tip with the c-clip facing upwards. The Gatan clip ring then fits in nicely
>> upside down, like a top hat, and snaps into place with the autogrid trapped
>> underneath. I tried this at room temperature and couldn't shake or tap it
>> out. We tested in cryo and the grid remained vitrified. There's some
>> restriction of the area accessible for tomography that might be remedied
>> with a larger hole in the clip ring.
>>
>> Try at your own risk, etc etc, but it seems to work.
>>
>> Michael
>>
>>
>> ------------------------------
>> *From:* 3dem [3dem-bounces at ncmir.ucsd.edu] on behalf of Wim Hagen [
>> hagen at embl.de]
>> *Sent:* Monday, June 17, 2019 19:16
>> *To:* 3dem at ncmir.ucsd.edu
>> *Subject:* Re: [3dem] side entry holder for autogrids?
>>
>> Hi Michael,
>>
>> I’ve discussed this with Chris Booth from Gatan some time ago, a multiple
>> cartridge side entry holder will for sure also help a lot of people with
>> side-entry screening systems, preparing for later data collection  in
>> central facilities. With other people’s ideas for cheap side-entry low-kv
>> screening tools, this could be quite a market.
>>
>> Best,
>>
>> Wim Hagen
>> EMBL Heidelberg
>>
>> On 17. Jun 2019, at 18:00, Michael Elbaum <michael.elbaum at weizmann.ac.il>
>> wrote:
>>
>> Hi all,    wondering if anyone has heard/thought/dreamed of a side entry
>> holder for clipped grids? This would be very useful in mixed company (of
>> microscopes, of course), and could enable analysis of grids post-tomography
>> by non-standard methods such as EDS. Perhaps someone has experience in
>> modifying a 626 tip for the purpose?
>> regards,
>> Michael
>>
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