[3dem] side entry holder for autogrids?

[Sharon G Wolf]

David
In our case it is the second type with the “feet” tool. That corresponds to the “626-70” holder. In the first case ( the tool with the threaded end) it’s the 626-60 holder. We have one of the 626-60 holders too,  but did this successful  test with the 626-70 holder only.
Sharon

Sent from my iPhone

On 26 Jun 2019, at 19:01, David Michael Belnap <David.Belnap at utah.edu<mailto:David.Belnap at utah.edu>> wrote:

Michael and Haifeng,

What type of the 626 holder are you using?

-One type has phosphor bronze clip ring that appears a bit slanted on the side that faces the grid.  The inside of the clip ring has threads.  The tool used to put them in place and remove them is a rod with a blunt end and a threaded end.

-The other type uses Be clip rings that are more flat.  The clip rings resemble the Krios clip rings in that the Be ring has a raised portion on the outer diameter.  The tool used to insert and remove them has small “feet” that the user spreads out to about 180 degrees to hook against the clip ring to manipulate it.

Thanks.

David


On Jun 25, 2019, at 3:18 PM, Haifeng He <haifenghe at gmail.com<mailto:haifenghe at gmail.com>> wrote:

I just tried what you said in the loading station only and it worked! The shutter also closes nicely. Great idea! Will still need to see if the drift rate is affected at Cryo temperature.

Haifeng

On Tue, Jun 25, 2019 at 3:49 PM Michael Elbaum <michael.elbaum at weizmann.ac.il<mailto:michael.elbaum at weizmann.ac.il>> wrote:
Regarding my earlier post about a side entry holder for autogrids, here's a simple hack. The autogrid cassette sits comfortably in the 626-70 holder tip with the c-clip facing upwards. The Gatan clip ring then fits in nicely upside down, like a top hat, and snaps into place with the autogrid trapped underneath. I tried this at room temperature and couldn't shake or tap it out. We tested in cryo and the grid remained vitrified. There's some restriction of the area accessible for tomography that might be remedied with a larger hole in the clip ring.

Try at your own risk, etc etc, but it seems to work.

Michael


________________________________
From: 3dem [3dem-bounces at ncmir.ucsd.edu<mailto:3dem-bounces at ncmir.ucsd.edu>] on behalf of Wim Hagen [hagen at embl.de<mailto:hagen at embl.de>]
Sent: Monday, June 17, 2019 19:16
To: 3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu>
Subject: Re: [3dem] side entry holder for autogrids?

Hi Michael,

I’ve discussed this with Chris Booth from Gatan some time ago, a multiple cartridge side entry holder will for sure also help a lot of people with side-entry screening systems, preparing for later data collection  in central facilities. With other people’s ideas for cheap side-entry low-kv screening tools, this could be quite a market.

Best,

Wim Hagen
EMBL Heidelberg

On 17. Jun 2019, at 18:00, Michael Elbaum <michael.elbaum at weizmann.ac.il<mailto:michael.elbaum at weizmann.ac.il>> wrote:

Hi all,    wondering if anyone has heard/thought/dreamed of a side entry holder for clipped grids? This would be very useful in mixed company (of microscopes, of course), and could enable analysis of grids post-tomography by non-standard methods such as EDS. Perhaps someone has experience in modifying a 626 tip for the purpose?
regards,
Michael
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