[3dem] Negative stain on graphene oxyde grids

[Andreas Boland]

Dear Julien,

If I am not mistaken this topic has been discussed already in the past. I think there is no good reason to use graphene oxide grids for negative stain. 

First, you won’t need any improvement in contrast of course and second the graphene oxide grids get damaged during this procedure. 

There are commercially available grids already pre-coated carbon that you can buy and are perfectly fine for negative stain.

Best wishes and good luck. Andreas 

> On 11 Sep 2018, at 16:55, Julien Bous <julien.bous at etu.umontpellier.fr> wrote:
> 
> Dear Community,
> 
> I'm PhD student in cryoEM and I plan to use graphene oxide grids for a complicate sample. Therefore, in order to make a first assessment of the interaction between the graphene oxide and my sample, I want to try in first to do a negative stain on graphene oxide with my sample Has anyone ever seen or tried this , I didn't find any bibliography? And is it looks like a good idea for more experienced peoples?
> 
> Thank you very much for your time.
> 
> Best,
> 
> Julien
> 
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