<html><head><meta http-equiv="Content-Type" content="text/html; charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">Dear Julien,<div class=""><br class=""></div><div class="">If I am not mistaken this topic has been discussed already in the past. I think there is no good reason to use graphene oxide grids for negative stain. </div><div class=""><br class=""></div><div class="">First, you won’t need any improvement in contrast of course and second the graphene oxide grids get damaged during this procedure. </div><div class=""><br class=""></div><div class="">There are commercially available grids already pre-coated carbon that you can buy and are perfectly fine for negative stain.</div><div class=""><br class=""></div><div class="">Best wishes and good luck. Andreas <br class=""><div class=""><div><br class=""><blockquote type="cite" class=""><div class="">On 11 Sep 2018, at 16:55, Julien Bous <<a href="mailto:julien.bous@etu.umontpellier.fr" class="">julien.bous@etu.umontpellier.fr</a>> wrote:</div><br class="Apple-interchange-newline"><div class="">
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<div text="#000000" bgcolor="#FFFFFF" class=""><p class="">Dear Community,</p><p class=""><span id="p115" class=""><span id="131" class="">I</span>'m PhD student in <span id="120" class="">cryoEM</span> and <span id="132" class="">I</span> <span id="211-212" class="">plan</span> to <span id="213-214" class="">use</span> <span id="134" class="">graphene</span> <span id="137" class="">oxide</span> grids
for a complicate sample.</span><span id="p228" class=""> Therefore, in
order to make a first assessment of the interaction between the
graphene oxide and my sample, I want to try in first to do a
negative stain on graphene oxide with my sample Has anyone ever
seen or tried this , I didn't find any bibliography?</span><span id="p229" class=""> And is it looks like a good idea for more experienced
peoples?</span></p><p class=""><span id="p229" class="">Thank you very much for your time.</span></p><p class=""><span id="p229" class="">Best,</span></p><p class=""><span id="p229" class="">Julien<br class="">
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