[3dem] [ccpem] lost gain reference image (Camera Normalization)

[Marin van Heel]

Dear Dimitry,

We have shown that you can perform movie alignments on a more local 
basis without using very extreme low-pass filters (sometimes described 
as "B-factors"). Thus you will necessarily have a larger yield of usable 
particles from the same set of micrographs. That is more than sufficient 
evidence of improvement!  The FRC is a metric that is local to your 
correction operation and that measures the improvement directly. The 
final 3D map resolution only comes at the end  of a long pipeline, that 
any two people will perform differently and that is too indirectly 
related to the very early data-set correction. Bottom line: the FRC 
metric is necessary and sufficient to show the data-set improvement by 
the camera correction. However it does not necessarily and sufficiently 
guarantee that nobody will generate gold-standard garbage further down 
the pipeline.  ;)
Cheers,
Marin

On 02/10/2018 07:54, Dimitry Tegunov wrote:
> Dear Marin,
>
> do you have results showing that the proposed correction improves the 
> final map resolution vs. conventionally gain-corrected movies? I think 
> the FRC curves are necessary and sufficient proof , but not sufficient 
> to prove the advantage of your approach.
>
> Cheers,
> Dimitry
>
> On Mon, Oct 1, 2018 at 9:23 PM Marin van Heel 
> <0000057a89ab08a1-dmarc-request at jiscmail.ac.uk 
> <mailto:0000057a89ab08a1-dmarc-request at jiscmail.ac.uk>> wrote:
>
>     Dear Da,
>
>     In IMAGIC-4D  you can perform the necessary camera correction!
>     (https://www.nature.com/articles/srep10317). It does it better
>     than any
>     manufactures correction and improves the data significantly even when
>     performed after using the standard gain correction.
>
>     Cheers,
>
>     Marin
>
>
>     =====================================================
>
>     On 01/10/2018 15:36, Da Cui wrote:
>     > Hi all,
>     >     The gain reference image for one dataset was missing by
>     accident. In order to achieve a more accurate motioncor result,
>     does anyone have idea about how to generate a gain reference image
>     from the dataset (around 3k movies)?
>     >     Thank you so much for your help!!!
>     > ---Da
>     >
>     >
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