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      Dear Dimitry,<br>
      <br>
      We have shown that you can perform movie alignments on a more
      local basis without using very extreme low-pass filters (sometimes
      described as "B-factors"). Thus you will necessarily have a larger
      yield of usable particles from the same set of micrographs. That
      is more than sufficient evidence of improvement!  The FRC is a
      metric that is local to your correction operation and that
      measures the improvement directly. The final 3D map resolution
      only comes at the end  of a long pipeline, that any two people
      will perform differently and that is too indirectly related to the
      very early data-set correction. Bottom line: the FRC metric is
      necessary and sufficient to show the data-set improvement by the
      camera correction. However it does not necessarily and
      sufficiently guarantee that nobody will generate gold-standard
      garbage further down the pipeline.  ;)<br>
      Cheers,<br>
      Marin<br>
      <br>
      On 02/10/2018 07:54, Dimitry Tegunov wrote:<br>
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    <blockquote type="cite"
cite="mid:CAL7Y77OYKX+QTmavUqof6CGiVgCC1jHdh5TSue3sPrrAvkubcg@mail.gmail.com">
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      <div dir="ltr">Dear Marin,
        <div><br>
        </div>
        <div>do you have results showing that the proposed correction
          improves the final map resolution vs. conventionally
          gain-corrected movies? I think the FRC curves are necessary
          and sufficient proof , but not sufficient to prove the
          advantage of your approach.</div>
        <div><br>
        </div>
        <div>Cheers,</div>
        <div>Dimitry</div>
      </div>
      <br>
      <div class="gmail_quote">
        <div dir="ltr">On Mon, Oct 1, 2018 at 9:23 PM Marin van Heel
          <<a
            href="mailto:0000057a89ab08a1-dmarc-request@jiscmail.ac.uk"
            moz-do-not-send="true">0000057a89ab08a1-dmarc-request@jiscmail.ac.uk</a>>
          wrote:<br>
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        <blockquote class="gmail_quote" style="margin:0 0 0
          .8ex;border-left:1px #ccc solid;padding-left:1ex">Dear Da,<br>
          <br>
          In IMAGIC-4D  you can perform the necessary camera correction!
          <br>
          (<a href="https://www.nature.com/articles/srep10317"
            rel="noreferrer" target="_blank" moz-do-not-send="true">https://www.nature.com/articles/srep10317</a>). 
          It does it better than any <br>
          manufactures correction and improves the data significantly
          even when <br>
          performed after using the standard gain correction.<br>
          <br>
          Cheers,<br>
          <br>
          Marin<br>
          <br>
          <br>
          =====================================================<br>
          <br>
          On 01/10/2018 15:36, Da Cui wrote:<br>
          > Hi all,<br>
          >     The gain reference image for one dataset was missing
          by accident. In order to achieve a more accurate motioncor
          result, does anyone have idea about how to generate a gain
          reference image from the dataset (around 3k movies)?<br>
          >     Thank you so much for your help!!!<br>
          > ---Da<br>
          ><br>
          >
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