[3dem] Ice thickness

[David Michael Belnap]

Sanchaita,

"There is no royal road to [success in cryo-EM].”  :-)

I would start with settings that have been successful with other samples on that Vitrobot.

David


On Feb 26, 2018, at 9:09 AM, Sacha De Carlo <sachadecarlo at yahoo.com<mailto:sachadecarlo at yahoo.com>> wrote:

Unfortunately the molecular weight of the complex alone is useless to determine “proper” settings for the vitrobot. Buffer composition, sample concentration, etc would be required to give you an educated guess ...

Cheers
Sacha


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On Monday, February 26, 2018, 5:05 PM, Sanchaita Das <sanchaita06 at gmail.com<mailto:sanchaita06 at gmail.com>> wrote:

Dear all,
Does anyone have suggestions about what vitrobot settings may work for getting good single particles for a protein that is aproox 300KDa?
I know its trial and error but if something might work for smaller proteins would love suggestions.
Best,
Sanchaita


I think 99  times and I find nothing.
I stop thinking,
swim in silence,
and the truth comes to me.

- Albert Einstein -

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