<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
</head>
<body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">
Sanchaita,
<div class=""><br class="">
</div>
<div class="">"There is no royal road to [success in cryo-EM].†:-)</div>
<div class=""><br class="">
</div>
<div class="">I would start with settings that have been successful with other samples on that Vitrobot.</div>
<div class=""><br class="">
</div>
<div class="">David</div>
<div class=""><br class="">
</div>
<div class=""><br class="">
<div>
<blockquote type="cite" class="">
<div class="">On Feb 26, 2018, at 9:09 AM, Sacha De Carlo <<a href="mailto:sachadecarlo@yahoo.com" class="">sachadecarlo@yahoo.com</a>> wrote:</div>
<br class="Apple-interchange-newline">
<div class=""><!--[if gte mso 9]><xml><o:OfficeDocumentSettings><o:AllowPNG/><o:PixelsPerInch>96</o:PixelsPerInch></o:OfficeDocumentSettings></xml><![endif]-->
<div class="">Unfortunately the molecular weight of the complex alone is useless to determine “proper†settings for the vitrobot. Buffer composition, sample concentration, etc would be required to give you an educated guess ...
<div class=""><br class="">
</div>
<div class="">Cheers</div>
<div class="">Sacha<br class="">
<br class="">
<br class="">
<a href="https://yho.com/footer0" class="">Sent from Yahoo Mail for iPhone</a><br class="">
<br class="">
<p class="yahoo-quoted-begin" style="font-size: 15px; color: #715FFA; padding-top: 15px; margin-top: 0">
On Monday, February 26, 2018, 5:05 PM, Sanchaita Das <<a href="mailto:sanchaita06@gmail.com" class="">sanchaita06@gmail.com</a>> wrote:</p>
<blockquote class="iosymail">
<div id="yiv4014431603" class="">
<div class="">Dear all,
<div class="">Does anyone have suggestions about what vitrobot settings may work for getting good single particles for a protein that is aproox 300KDa?</div>
<div class="">I know its trial and error but if something might work for smaller proteins would love suggestions.</div>
<div class="">Best,</div>
<div class="">Sanchaita<br class="">
<br class="">
<div class=""><br class="">
I think 99 times and I find nothing.<br class="">
I stop thinking,<br class="">
swim in silence,<br class="">
and the truth comes to me.<br class="">
<br class="">
- Albert Einstein -<br class="">
</div>
</div>
</div>
</div>
_______________________________________________<br class="">
3dem mailing list<br class="">
<a ymailto="mailto:3dem@ncmir.ucsd.edu" href="mailto:3dem@ncmir.ucsd.edu" class="">3dem@ncmir.ucsd.edu</a><br class="">
<a href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem" target="_blank" class="">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a><br class="">
<blockquote class=""></blockquote>
</blockquote>
</div>
</div>
_______________________________________________<br class="">
3dem mailing list<br class="">
<a href="mailto:3dem@ncmir.ucsd.edu" class="">3dem@ncmir.ucsd.edu</a><br class="">
https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem<br class="">
</div>
</blockquote>
</div>
<br class="">
</div>
</body>
</html>