[3dem] Ice thickness

[Sacha De Carlo]

Unfortunately the molecular weight of the complex alone is useless to determine “proper” settings for the vitrobot. Buffer composition, sample concentration, etc would be required to give you an educated guess ...
CheersSacha


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On Monday, February 26, 2018, 5:05 PM, Sanchaita Das <sanchaita06 at gmail.com> wrote:

Dear all,Does anyone have suggestions about what vitrobot settings may work for getting good single particles for a protein that is aproox 300KDa?I know its trial and error but if something might work for smaller proteins would love suggestions.Best,Sanchaita


I think 99  times and I find nothing.
I stop thinking,
swim in silence,
and the truth comes to me.

- Albert Einstein -
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