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Unfortunately the molecular weight of the complex alone is useless to determine “proper” settings for the vitrobot. Buffer composition, sample concentration, etc would be required to give you an educated guess ...<div><br></div><div>Cheers</div><div>Sacha<br><br><br><a href="https://yho.com/footer0">Sent from Yahoo Mail for iPhone</a><br><br><p class="yahoo-quoted-begin" style="font-size: 15px; color: #715FFA; padding-top: 15px; margin-top: 0">On Monday, February 26, 2018, 5:05 PM, Sanchaita Das <sanchaita06@gmail.com> wrote:</p><blockquote class="iosymail"><div id="yiv4014431603"><div>Dear all,<div>Does anyone have suggestions about what vitrobot settings may work for getting good single particles for a protein that is aproox 300KDa?</div><div>I know its trial and error but if something might work for smaller proteins would love suggestions.</div><div>Best,</div><div>Sanchaita<br><br><div><br>I think 99 times and I find nothing.<br>I stop thinking,<br>swim in silence,<br>and the truth comes to me.<br><br>- Albert Einstein -<br> </div></div></div></div>_______________________________________________<br>3dem mailing list<br><a ymailto="mailto:3dem@ncmir.ucsd.edu" href="mailto:3dem@ncmir.ucsd.edu">3dem@ncmir.ucsd.edu</a><br><a href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem" target="_blank">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a><br><blockquote></blockquote></blockquote></div>
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