[3dem] Gatan 914 holder - should shutter close further than this?

Sharon G Wolf Sharon.Wolf at weizmann.ac.il
Fri Aug 10 07:51:18 PDT 2018


We had leopard ice on our 914 holder all the time, and we had to send it in for repair in order for the problem to be solved. The shutter must close all the way as well.

Sent from my iPhone

On 10 Aug 2018, at 16:53, Wim Hagen <hagen at embl.de<mailto:hagen at embl.de>> wrote:

Dear Teige,

Leopard print is typically caused by temperature problems, when the holder not being baked properly, or the clipping not making full contact.
Shutter problems would show contamination by large ice crystals picked up during holder insertion through air with the shutter not completely shielding.

Best,

Wim Hagen
EMBL Heidelberg

On Aug 10, 2018, at 3:44 PM, Matthews-Palmer, Teige Rowan Seal <t.matthews-palmer14 at imperial.ac.uk<mailto:t.matthews-palmer14 at imperial.ac.uk>> wrote:

Dear 3DEMers,

While troubleshooting some “leopard print” ice contamination on a Gatan 914 holder, we noticed that the underside of the cryoshield/shutter does not close 'all the way' (you can see the grid).
I wanted to know from other users of 914 holders, does the underside of your shutter look like this when closed, or does it slide further to completely seal away the grid?

An alternative cause of the contamination is a leak through the shutter rod assembly.
Any thoughts welcome!
Thanks,
Teige

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