[3dem] gold fiducials

Oleksiy Kovtun oleksiy.kovtun at embl.de
Mon Sep 18 09:16:54 PDT 2017

Hi Sharon, 
is this PEG-nanogold available for purchase somewhere? 
Thank you, 
On Thu, Sep 14, 2017 at 15:35, Sharon G Wolf  wrote: 
 Hi Daniel,
 I can confirm that these gold fiducials  produced by Laurence Duchesne behave very nicely. We use them routinely here. 
  They do not usually stick to macromolecular  or cell preps. 
  From: 3dem [mailto:3dem-bounces at ncmir.ucsd.edu (mailto:3dem-bounces at ncmir.ucsd.edu)] On Behalf Of dchretie
Sent: Thursday, September 14, 2017 16:35
To: 3dem at ncmir.ucsd.edu (mailto:3dem at ncmir.ucsd.edu)
Subject: Re: [3dem] gold fiducials
 Dear Daniel,
 We routinely use 10 nm gold NPs coated with a mixed PEG-peptidol matrix prepared according to :
 Robust ligand shells for biological applications of gold nanoparticles 
Duchesne L., Gentili D., Comes-Franchini M. and D.G. Fernig
Langmuir, 2008, 24:13572-13580
 We rarely see interactions with proteins. They occasionally tend to stick to some types of lipid vesicles, notably at the air-water interfaces.
 Best wishes,
 Le 14 sept. 2017 à 14:35, Wright, Elizabeth R.  a écrit :
 Hi Daniel,
 We use these…
 BSA-treated tracers, EM grade (EMS, cat. no. 25486).
 You can also make your own BSA-treated gold, protocols outlined in Iancu et al., Nature Protocols (2006).
 Good luck!
 Elizabeth R. Wright, PhD
 Associate Professor
 Emory University
 (404) 727-4665
 erwrigh at emory.edu (mailto:erwrigh at emory.edu)
 From: 3dem  on behalf of Levy Daniel 
Date: Thursday, September 14, 2017 at 8:03 AM
To: "3dem at ncmir.ucsd.edu (mailto:3dem at ncmir.ucsd.edu)" 
Subject: [3dem] gold fiducials
 Dear All,
 The 10 nm ProteinA gold fiducials that we use in cryo-ET experiments strongly interact with one of our proteins of interest. Do you have any suggestions for a 10 or 6nm gold fiducials ?
 Daniel Lévy
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