[3dem] gold fiducials

[Oleksiy Kovtun]

Hi Sharon, 
is this PEG-nanogold available for purchase somewhere? 
Thank you, 
Oleksiy
On Thu, Sep 14, 2017 at 15:35, Sharon G Wolf  wrote: 
 Hi Daniel,
 I can confirm that these gold fiducials  produced by Laurence Duchesne behave very nicely. We use them routinely here. 
  They do not usually stick to macromolecular  or cell preps. 
 Best,
 Sharon
  From: 3dem [mailto:3dem-bounces at ncmir.ucsd.edu (mailto:3dem-bounces at ncmir.ucsd.edu)] On Behalf Of dchretie
Sent: Thursday, September 14, 2017 16:35
To: 3dem at ncmir.ucsd.edu (mailto:3dem at ncmir.ucsd.edu)
Subject: Re: [3dem] gold fiducials
 Dear Daniel,
 We routinely use 10 nm gold NPs coated with a mixed PEG-peptidol matrix prepared according to :
 Robust ligand shells for biological applications of gold nanoparticles 
Duchesne L., Gentili D., Comes-Franchini M. and D.G. Fernig
Langmuir, 2008, 24:13572-13580
 We rarely see interactions with proteins. They occasionally tend to stick to some types of lipid vesicles, notably at the air-water interfaces.
 Best wishes,
 Denis
 Le 14 sept. 2017 à 14:35, Wright, Elizabeth R.  a écrit :
 Hi Daniel,
 We use these…
 BSA-treated tracers, EM grade (EMS, cat. no. 25486).
 You can also make your own BSA-treated gold, protocols outlined in Iancu et al., Nature Protocols (2006).
 Good luck!
 Liz
 ----
 Elizabeth R. Wright, PhD
 Associate Professor
 Emory University
 (404) 727-4665
 erwrigh at emory.edu (mailto:erwrigh at emory.edu)
 From: 3dem  on behalf of Levy Daniel 
Date: Thursday, September 14, 2017 at 8:03 AM
To: "3dem at ncmir.ucsd.edu (mailto:3dem at ncmir.ucsd.edu)" 
Subject: [3dem] gold fiducials
 Dear All,
 The 10 nm ProteinA gold fiducials that we use in cryo-ET experiments strongly interact with one of our proteins of interest. Do you have any suggestions for a 10 or 6nm gold fiducials ?
 Best
 Daniel Lévy
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