<!DOCTYPE html><html><head><meta http-equiv="Content-Type" content="text/html; charset=utf-8" /></head><body><div data-crea="font-wrapper" style="font-family: Tahoma; font-size: 16px; direction: ltr"><div style="font-family: Tahoma; font-size: 16px"></div>Hi Sharon, <div>is this PEG-nanogold available for purchase somewhere? </div><div><br></div><div>Thank you, </div><div> </div><div>Oleksiy<br><div><br><div></div><br><br><div data-anchor="reply-title">On Thu, Sep 14, 2017 at 15:35, Sharon G Wolf <sharon.wolf@weizmann.ac.il> wrote:</div><blockquote><div xmlns:v="urn:schemas-microsoft-com:vml" xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:m="http://schemas.microsoft.com/office/2004/12/omml" xmlns="http://www.w3.org/TR/REC-html40"><div lang="EN-US" link="blue" vlink="purple">



<div>
<span style='font-size: 11.0pt;font-family: "Calibri",sans-serif;color: #1F497D'>Hi Daniel,</span><br><br><br><span style='font-size: 11.0pt;font-family: "Calibri",sans-serif;color: #1F497D'>I can confirm that these gold fiducials  produced by Laurence Duchesne behave very nicely. We use them routinely here.
</span><br><br><br><span style='font-size: 11.0pt;font-family: "Calibri",sans-serif;color: #1F497D'> They do not usually stick to macromolecular  or cell preps.
</span><br><br><br><span style='font-size: 11.0pt;font-family: "Calibri",sans-serif;color: #1F497D'>Best,</span><br><br><br><span style='font-size: 11.0pt;font-family: "Calibri",sans-serif;color: #1F497D'>Sharon</span><br><br><br><span style='font-size: 11.0pt;font-family: "Calibri",sans-serif;color: #1F497D'></span><br> <br><br><a name="_MailEndCompose" target="_blank" tabindex="-1"><span style='font-size: 11.0pt;font-family: "Calibri",sans-serif;color: #1F497D'></span></a><br><a name="_MailEndCompose" target="_blank" tabindex="-1"> </a><br><br><div>
<div style="border: none;border-top: solid #E1E1E1 1.0pt;padding: 3.0pt 0in 0in 0in">
<b><span style='font-size: 11.0pt;font-family: "Calibri",sans-serif'>From:</span></b><span style='font-size: 11.0pt;font-family: "Calibri",sans-serif'> 3dem [mailto:<a href="mailto:3dem-bounces@ncmir.ucsd.edu" target="_blank" tabindex="-1" rel="external">3dem-bounces@ncmir.ucsd.edu</a>]
<b>On Behalf Of </b>dchretie<br><b>Sent:</b> Thursday, September 14, 2017 16:35<br><b>To:</b> <a href="mailto:3dem@ncmir.ucsd.edu" target="_blank" tabindex="-1" rel="external">3dem@ncmir.ucsd.edu</a><br><b>Subject:</b> Re: [3dem] gold fiducials</span><br><br><br></div>
</div>
<br> <br><br>
Dear Daniel,<br><br><br><div>
<br> <br><br></div>
<div>
We routinely use 10 nm gold NPs coated with a mixed PEG-peptidol matrix prepared according to :<br><br><br></div>
<div>
<br> <br><br></div>
<div>
<span>Robust ligand shells for biological applications of gold nanoparticles</span>
<br><span>Duchesne L</span>., Gentili D., Comes-Franchini M. and D.G. Fernig<br><span>Langmuir</span>, 2008, 24:13572-13580<br><br><br></div>
<div>
<br> <br><br></div>
<div>
We rarely see interactions with proteins. They occasionally tend to stick to some types of lipid vesicles, notably at the air-water interfaces.<br><br><br></div>
<div>
<br> <br><br></div>
<div>
Best wishes,<br><br><br></div>
<div>
<br> <br><br></div>
<div>
Denis<br><br><br></div>
<div>
<br> <br><br><div>
<blockquote style="margin-top: 5.0pt;margin-bottom: 5.0pt">
<div>
Le 14 sept. 2017 à 14:35, Wright, Elizabeth R. <<a href="mailto:erwrigh@emory.edu" target="_blank" tabindex="-1" rel="external">erwrigh@emory.edu</a>> a écrit :<br><br><br></div>
<br> <br><br><div>
<div>
<span style='font-family: "Arial",sans-serif'>Hi Daniel,</span><span style='font-size: 11.0pt;font-family: "Calibri",sans-serif'></span><br><br><br></div>
<div>
<span style='font-family: "Arial",sans-serif'> </span><span style='font-size: 11.0pt;font-family: "Calibri",sans-serif'></span><br><br><br></div>
<div>
<span style='font-family: "Arial",sans-serif'>We use these…</span><span style='font-size: 11.0pt;font-family: "Calibri",sans-serif'></span><br><br><br></div>
<div>
<span style='font-family: "Arial",sans-serif'>BSA-treated tracers, EM grade (EMS, cat. no. 25486).</span><span style='font-size: 11.0pt;font-family: "Calibri",sans-serif'></span><br><br><br></div>
<div>
<span style='font-family: "Arial",sans-serif'>You can also make your own BSA-treated gold, protocols outlined in Iancu et al., Nature Protocols (2006).</span><span style='font-size: 11.0pt;font-family: "Calibri",sans-serif'></span><br><br><br></div>
<div>
<span style='font-family: "Arial",sans-serif'> </span><span style='font-size: 11.0pt;font-family: "Calibri",sans-serif'></span><br><br><br></div>
<div>
<span style='font-family: "Arial",sans-serif'>Good luck!</span><span style='font-size: 11.0pt;font-family: "Calibri",sans-serif'></span><br><br><br></div>
<div>
<span style='font-family: "Arial",sans-serif'> </span><span style='font-size: 11.0pt;font-family: "Calibri",sans-serif'></span><br><br><br></div>
<div>
<span style='font-family: "Arial",sans-serif'>Liz</span><span style='font-size: 11.0pt;font-family: "Calibri",sans-serif'></span><br><br><br></div>
<div>
<span style='font-family: "Arial",sans-serif'> </span><span style='font-size: 11.0pt;font-family: "Calibri",sans-serif'></span><br><br><br></div>
<div>
<div>
----<span style='font-size: 11.0pt;font-family: "Calibri",sans-serif'></span><br><br><br></div>
<div>
<span style='font-family: "Arial",sans-serif'>Elizabeth R. Wright, PhD</span><span style='font-size: 11.0pt;font-family: "Calibri",sans-serif'></span><br><br><br></div>
<div>
<span style='font-family: "Arial",sans-serif'>Associate Professor</span><span style='font-size: 11.0pt;font-family: "Calibri",sans-serif'></span><br><br><br></div>
<div>
<span style='font-family: "Arial",sans-serif'>Emory University</span><span style='font-size: 11.0pt;font-family: "Calibri",sans-serif'></span><br><br><br></div>
<div>
<span style='font-family: "Arial",sans-serif'>(404) 727-4665</span><span style='font-size: 11.0pt;font-family: "Calibri",sans-serif'></span><br><br><br></div>
<div>
<span style='font-family: "Arial",sans-serif'><a href="mailto:erwrigh@emory.edu" target="_blank" tabindex="-1" rel="external"><span style="color: purple">erwrigh@emory.edu</span></a></span><span style='font-size: 11.0pt;font-family: "Calibri",sans-serif'></span><br><br><br></div>
</div>
<div>
<span style='font-family: "Arial",sans-serif'> </span><span style='font-size: 11.0pt;font-family: "Calibri",sans-serif'></span><br><br><br></div>
<div>
<span style='font-family: "Arial",sans-serif'> </span><span style='font-size: 11.0pt;font-family: "Calibri",sans-serif'></span><br><br><br></div>
<div style="border: none;border-top: solid #B5C4DF 1.0pt;padding: 3.0pt 0in 0in 0in">
<div>
<b><span style='font-family: "Calibri",sans-serif'>From:<span> </span></span></b><span style='font-family: "Calibri",sans-serif'>3dem <<a href="mailto:3dem-bounces@ncmir.ucsd.edu" target="_blank" tabindex="-1" rel="external"><span style="color: purple">3dem-bounces@ncmir.ucsd.edu</span></a>>
 on behalf of Levy Daniel <<a href="mailto:Daniel.Levy@curie.fr" target="_blank" tabindex="-1" rel="external"><span style="color: purple">Daniel.Levy@curie.fr</span></a>><br><b>Date:<span> </span></b>Thursday, September 14, 2017 at 8:03 AM<br><b>To:<span> </span></b>"<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank" tabindex="-1" rel="external"><span style="color: purple">3dem@ncmir.ucsd.edu</span></a>" <<a href="mailto:3dem@ncmir.ucsd.edu" target="_blank" tabindex="-1" rel="external"><span style="color: purple">3dem@ncmir.ucsd.edu</span></a>><br><b>Subject:<span> </span></b>[3dem] gold fiducials</span><span style='font-size: 11.0pt;font-family: "Calibri",sans-serif'></span><br><br><br></div>
</div>
<div>
<div>
<span style='font-size: 11.0pt;font-family: "Calibri",sans-serif'> </span><br><br><br></div>
</div>
<div>
<span lang="FR" style='font-size: 11.0pt;font-family: "Calibri",sans-serif'>Dear All,</span><span style='font-size: 11.0pt;font-family: "Calibri",sans-serif'></span><br><br><br></div>
<div>
<span style='font-size: 11.0pt;font-family: "Calibri",sans-serif'>The 10 nm ProteinA gold fiducials that we use in cryo-ET experiments strongly interact with one of our proteins of interest. Do you have any suggestions
 for a 10 or 6nm gold fiducials ?</span><br><br><br></div>
<div>
<span style='font-size: 11.0pt;font-family: "Calibri",sans-serif'>Best</span><br><br><br></div>
<div>
<span style='font-size: 11.0pt;font-family: "Calibri",sans-serif'>Daniel Lévy</span><br><br><br></div>
<br> <br><br><div align="center" style="text-align: center">
<hr size="2" width="100%" align="center"></div>
<span style='font-size: 7.5pt;font-family: "Arial",sans-serif;color: gray;background: white'><br>
This e-mail message (including any attachments) is for the sole use of<br>
the intended recipient(s) and may contain confidential and privileged<br>
information. If the reader of this message is not the intended<br>
recipient, you are hereby notified that any dissemination, distribution<br>
or copying of this message (including any attachments) is strictly<br>
prohibited.<br><br>
If you have received this message in error, please contact<br>
the sender by reply e-mail message and destroy all copies of the<br>
original message (including attachments).<br></span><span style='font-size: 9.0pt;font-family: "Helvetica",sans-serif;background: white'>_______________________________________________</span><span style='font-size: 9.0pt;font-family: "Helvetica",sans-serif'><br><span style="background: white">3dem mailing list</span><br></span><a href="mailto:3dem@ncmir.ucsd.edu" target="_blank" tabindex="-1" rel="external"><span style='font-size: 9.0pt;font-family: "Helvetica",sans-serif;color: purple;background: white'>3dem@ncmir.ucsd.edu</span></a><span style='font-size: 9.0pt;font-family: "Helvetica",sans-serif'><br></span><a href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem" target="_blank" tabindex="-1" rel="external"><span style='font-size: 9.0pt;font-family: "Helvetica",sans-serif;color: purple;background: white'>https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</span></a><br><br><br></div>
</blockquote>
</div>
<br> <br><br><div>
<div>
<div>
<div>
<span style="color: black">___________________________<br>
Université de Rennes 1<br>
UMR 6290, équipe TIPs<br>
Campus de Beaulieu, Bât. 13<br>
35042 Rennes Cedex<br>
France<br><br>
Tel. +33 (0)2 23 23 67 64<br><a href="http://equipes.igdr.univ-rennes1.fr/fr/tips/" target="_blank" tabindex="-1" rel="external">http://equipes.igdr.univ-rennes1.fr/en/tips/</a><br>
___________________________</span><br><br><br></div>
<div>
<span style="color: black"></span><br> <br><br></div>
</div>
<span style="color: black"></span><br> <br><br></div>
<br> <br><br></div>
<br> <br><br></div>
</div>


</div></div></blockquote></div></div></div></body></html>