[3dem] gold fiducials

Tue Sep 26 14:59:57 PDT 2017

Hi Oleksiy,

We do carry 10nm or other size gold covered with PEG. It does not contain “peptidol”, but it disperses exceptionally well and has no surface charge. It is not on our website yet, so contact me off list if interested. 

In my days doing tomography, I did try BSA-gold, Protein-A gold, even IgG-gold, besides unconjugated colloids. Protein-stabilized gold worked very well, unless there was an interaction like described in the OP.

Best wishes,

Vlad

Vlad Speransky, PhD

Life Sciences Product Specialist

Ted Pella, Inc.

 <http://www.tedpella.com/> http://www.tedpella.com/ 

530-243-2200 ext. 266

Cell 530-768-3953

 <mailto:vlad_speransky at tedpella.com> vlad_speransky at tedpella.com

From: 3dem [mailto:3dem-bounces at ncmir.ucsd.edu] On Behalf Of Oleksiy Kovtun
Sent: Monday, September 18, 2017 9:17 AM
To: Sharon G Wolf
Cc: 3dem at ncmir.ucsd.edu
Subject: Re: [3dem] gold fiducials

Hi Sharon, 

is this PEG-nanogold available for purchase somewhere? 

Thank you, 

Oleksiy

On Thu, Sep 14, 2017 at 15:35, Sharon G Wolf <sharon.wolf at weizmann.ac.il> wrote:

Hi Daniel,

I can confirm that these gold fiducials  produced by Laurence Duchesne behave very nicely. We use them routinely here. 

 They do not usually stick to macromolecular  or cell preps. 

Best,

Sharon

From: 3dem [mailto:3dem-bounces at ncmir.ucsd.edu] On Behalf Of dchretie
Sent: Thursday, September 14, 2017 16:35
To: 3dem at ncmir.ucsd.edu
Subject: Re: [3dem] gold fiducials

Dear Daniel,

We routinely use 10 nm gold NPs coated with a mixed PEG-peptidol matrix prepared according to :

Robust ligand shells for biological applications of gold nanoparticles 
Duchesne L., Gentili D., Comes-Franchini M. and D.G. Fernig
Langmuir, 2008, 24:13572-13580

We rarely see interactions with proteins. They occasionally tend to stick to some types of lipid vesicles, notably at the air-water interfaces.

Best wishes,

Denis

Le 14 sept. 2017 à 14:35, Wright, Elizabeth R. <erwrigh at emory.edu> a écrit :

Hi Daniel,

We use these…

BSA-treated tracers, EM grade (EMS, cat. no. 25486).

You can also make your own BSA-treated gold, protocols outlined in Iancu et al., Nature Protocols (2006).

Good luck!

Liz

----

Elizabeth R. Wright, PhD

Associate Professor

Emory University

(404) 727-4665

 <mailto:erwrigh at emory.edu> erwrigh at emory.edu

From: 3dem < <mailto:3dem-bounces at ncmir.ucsd.edu> 3dem-bounces at ncmir.ucsd.edu> on behalf of Levy Daniel < <mailto:Daniel.Levy at curie.fr> Daniel.Levy at curie.fr>
Date: Thursday, September 14, 2017 at 8:03 AM
To: " <mailto:3dem at ncmir.ucsd.edu> 3dem at ncmir.ucsd.edu" < <mailto:3dem at ncmir.ucsd.edu> 3dem at ncmir.ucsd.edu>
Subject: [3dem] gold fiducials

Dear All,

The 10 nm ProteinA gold fiducials that we use in cryo-ET experiments strongly interact with one of our proteins of interest. Do you have any suggestions for a 10 or 6nm gold fiducials ?

Best

Daniel Lévy

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