[3dem] gold fiducials

[dchretie]

Thanks Sharon ;-)

You can see their use in the following paper, both as fiducials and as mono-funcionalized NPs:

EB1 interacts with outwardly curved and straight regions of the microtubule lattice
Guesdon A., Bazile F., Buey R.M., Mohan R., Monier S., Rodríguez García R., Angevin M., Heichette C., Wieneke R., Tampé R., Duchesne L., Akhmanova A., Steinmetz M.O., and D. Chrétien
Nature Cell Biology, 2016, Sept 12

We can send aliquots of fiducials for those who want to test them.

best,

Denis

> Le 14 sept. 2017 à 16:34, Sharon G Wolf <Sharon.Wolf at weizmann.ac.il> a écrit :
> 
> Hi Daniel,
> I can confirm that these gold fiducials  produced by Laurence Duchesne behave very nicely. We use them routinely here.
>  They do not usually stick to macromolecular  or cell preps.
> Best,
> Sharon
>  
>   <>
> From: 3dem [mailto:3dem-bounces at ncmir.ucsd.edu <mailto:3dem-bounces at ncmir.ucsd.edu>] On Behalf Of dchretie
> Sent: Thursday, September 14, 2017 16:35
> To: 3dem at ncmir.ucsd.edu <mailto:3dem at ncmir.ucsd.edu>
> Subject: Re: [3dem] gold fiducials
>  
> Dear Daniel,
>  
> We routinely use 10 nm gold NPs coated with a mixed PEG-peptidol matrix prepared according to :
>  
> Robust ligand shells for biological applications of gold nanoparticles 
> Duchesne L., Gentili D., Comes-Franchini M. and D.G. Fernig
> Langmuir, 2008, 24:13572-13580
>  
> We rarely see interactions with proteins. They occasionally tend to stick to some types of lipid vesicles, notably at the air-water interfaces.
>  
> Best wishes,
>  
> Denis
>  
> Le 14 sept. 2017 à 14:35, Wright, Elizabeth R. <erwrigh at emory.edu <mailto:erwrigh at emory.edu>> a écrit :
>  
> Hi Daniel,
>  
> We use these…
> BSA-treated tracers, EM grade (EMS, cat. no. 25486).
> You can also make your own BSA-treated gold, protocols outlined in Iancu et al., Nature Protocols (2006).
>  
> Good luck!
>  
> Liz
>  
> ----
> Elizabeth R. Wright, PhD
> Associate Professor
> Emory University
> (404) 727-4665
> erwrigh at emory.edu <mailto:erwrigh at emory.edu>
>  
>  
> From: 3dem <3dem-bounces at ncmir.ucsd.edu <mailto:3dem-bounces at ncmir.ucsd.edu>> on behalf of Levy Daniel <Daniel.Levy at curie.fr <mailto:Daniel.Levy at curie.fr>>
> Date: Thursday, September 14, 2017 at 8:03 AM
> To: "3dem at ncmir.ucsd.edu <mailto:3dem at ncmir.ucsd.edu>" <3dem at ncmir.ucsd.edu <mailto:3dem at ncmir.ucsd.edu>>
> Subject: [3dem] gold fiducials
>  
> Dear All,
> The 10 nm ProteinA gold fiducials that we use in cryo-ET experiments strongly interact with one of our proteins of interest. Do you have any suggestions for a 10 or 6nm gold fiducials ?
> Best
> Daniel Lévy
>  
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