<html><head><meta http-equiv="Content-Type" content="text/html charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">Thanks Sharon ;-)<div class=""><br class=""></div><div class="">You can see their use in the following paper, both as fiducials and as mono-funcionalized NPs:</div><div class=""><br class=""></div><div class=""><span class="bold">EB1 interacts with outwardly curved and straight regions of the microtubule lattice</span><br class=""> <span class="bold">Guesdon A</span>., <span class="bold">Bazile F</span>., Buey R.M., Mohan R., <span class="bold">Monier S</span>., Rodríguez García R., <span class="bold">Angevin M</span>., <span class="bold">Heichette C</span>., Wieneke R., Tampé R., <span class="bold">Duchesne L</span>., Akhmanova A., Steinmetz M.O., and <span class="bold">D. Chrétien</span><br class=""> <span class="italic">Nature Cell Biology</span>, 2016, Sept 12</div><div class=""><br class=""></div><div class="">We can send aliquots of fiducials for those who want to test them.</div><div class=""><br class=""></div><div class="">best,</div><div class=""><br class=""></div><div class="">Denis</div><div class=""><br class=""><div><blockquote type="cite" class=""><div class="">Le 14 sept. 2017 à 16:34, Sharon G Wolf <<a href="mailto:Sharon.Wolf@weizmann.ac.il" class="">Sharon.Wolf@weizmann.ac.il</a>> a écrit :</div><br class="Apple-interchange-newline"><div class=""><div class="WordSection1" style="page: WordSection1; font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px;"><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">Hi Daniel,<o:p class=""></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">I can confirm that these gold fiducials produced by Laurence Duchesne behave very nicely. We use them routinely here.<o:p class=""></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""> They do not usually stick to macromolecular or cell preps.<o:p class=""></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">Best,<o:p class=""></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">Sharon<o:p class=""></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""><o:p class=""> </o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><a name="_MailEndCompose" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""><o:p class=""> </o:p></span></a></div><div class=""><div style="border-style: solid none none; border-top-width: 1pt; border-top-color: rgb(225, 225, 225); padding: 3pt 0in 0in;" class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><b class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class="">From:</span></b><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span class="Apple-converted-space"> </span>3dem [<a href="mailto:3dem-bounces@ncmir.ucsd.edu" style="color: purple; text-decoration: underline;" class="">mailto:3dem-bounces@ncmir.ucsd.edu</a>]<span class="Apple-converted-space"> </span><b class="">On Behalf Of<span class="Apple-converted-space"> </span></b>dchretie<br class=""><b class="">Sent:</b><span class="Apple-converted-space"> </span>Thursday, September 14, 2017 16:35<br class=""><b class="">To:</b><span class="Apple-converted-space"> </span><a href="mailto:3dem@ncmir.ucsd.edu" style="color: purple; text-decoration: underline;" class="">3dem@ncmir.ucsd.edu</a><br class=""><b class="">Subject:</b><span class="Apple-converted-space"> </span>Re: [3dem] gold fiducials<o:p class=""></o:p></span></div></div></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">Dear Daniel,<o:p class=""></o:p></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">We routinely use 10 nm gold NPs coated with a mixed PEG-peptidol matrix prepared according to :<o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span class="bold">Robust ligand shells for biological applications of gold nanoparticles</span><span class="Apple-converted-space"> </span><br class=""><span class="bold">Duchesne L</span>., Gentili D., Comes-Franchini M. and D.G. Fernig<br class=""><span class="italic">Langmuir</span>, 2008, 24:13572-13580<o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">We rarely see interactions with proteins. They occasionally tend to stick to some types of lipid vesicles, notably at the air-water interfaces.<o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">Best wishes,<o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">Denis<o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div><div class=""><blockquote style="margin-top: 5pt; margin-bottom: 5pt;" class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">Le 14 sept. 2017 à 14:35, Wright, Elizabeth R. <<a href="mailto:erwrigh@emory.edu" style="color: purple; text-decoration: underline;" class="">erwrigh@emory.edu</a>> a écrit :<o:p class=""></o:p></div></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div><div class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; background-color: white;" class=""><span style="font-family: Arial, sans-serif;" class="">Hi Daniel,</span><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; background-color: white;" class=""><span style="font-family: Arial, sans-serif;" class=""> </span><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; background-color: white;" class=""><span style="font-family: Arial, sans-serif;" class="">We use these…</span><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; background-color: white;" class=""><span style="font-family: Arial, sans-serif;" class="">BSA-treated tracers, EM grade (EMS, cat. no. 25486).</span><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; background-color: white;" class=""><span style="font-family: Arial, sans-serif;" class="">You can also make your own BSA-treated gold, protocols outlined in Iancu et al., Nature Protocols (2006).</span><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; background-color: white;" class=""><span style="font-family: Arial, sans-serif;" class=""> </span><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; background-color: white;" class=""><span style="font-family: Arial, sans-serif;" class="">Good luck!</span><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; background-color: white;" class=""><span style="font-family: Arial, sans-serif;" class=""> </span><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; background-color: white;" class=""><span style="font-family: Arial, sans-serif;" class="">Liz</span><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; background-color: white;" class=""><span style="font-family: Arial, sans-serif;" class=""> </span><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""></o:p></span></div></div><div class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; background-color: white;" class="">----<span style="font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; background-color: white;" class=""><span style="font-family: Arial, sans-serif;" class="">Elizabeth R. Wright, PhD</span><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; background-color: white;" class=""><span style="font-family: Arial, sans-serif;" class="">Associate Professor</span><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; background-color: white;" class=""><span style="font-family: Arial, sans-serif;" class="">Emory University</span><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; background-color: white;" class=""><span style="font-family: Arial, sans-serif;" class="">(404) 727-4665</span><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; background-color: white;" class=""><span style="font-family: Arial, sans-serif;" class=""><a href="mailto:erwrigh@emory.edu" style="color: purple; text-decoration: underline;" class=""><span style="color: purple;" class="">erwrigh@emory.edu</span></a></span><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""></o:p></span></div></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; background-color: white;" class=""><span style="font-family: Arial, sans-serif;" class=""> </span><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; background-color: white;" class=""><span style="font-family: Arial, sans-serif;" class=""> </span><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""></o:p></span></div></div><div style="border-style: solid none none; border-top-width: 1pt; border-top-color: rgb(181, 196, 223); padding: 3pt 0in 0in;" class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; background-color: white;" class=""><b class=""><span style="font-family: Calibri, sans-serif;" class="">From:<span class="apple-converted-space"> </span></span></b><span style="font-family: Calibri, sans-serif;" class="">3dem <<a href="mailto:3dem-bounces@ncmir.ucsd.edu" style="color: purple; text-decoration: underline;" class=""><span style="color: purple;" class="">3dem-bounces@ncmir.ucsd.edu</span></a>> on behalf of Levy Daniel <<a href="mailto:Daniel.Levy@curie.fr" style="color: purple; text-decoration: underline;" class=""><span style="color: purple;" class="">Daniel.Levy@curie.fr</span></a>><br class=""><b class="">Date:<span class="apple-converted-space"> </span></b>Thursday, September 14, 2017 at 8:03 AM<br class=""><b class="">To:<span class="apple-converted-space"> </span></b>"<a href="mailto:3dem@ncmir.ucsd.edu" style="color: purple; text-decoration: underline;" class=""><span style="color: purple;" class="">3dem@ncmir.ucsd.edu</span></a>" <<a href="mailto:3dem@ncmir.ucsd.edu" style="color: purple; text-decoration: underline;" class=""><span style="color: purple;" class="">3dem@ncmir.ucsd.edu</span></a>><br class=""><b class="">Subject:<span class="apple-converted-space"> </span></b>[3dem] gold fiducials</span><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""></o:p></span></div></div></div><div class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; background-color: white;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class=""> <o:p class=""></o:p></span></div></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; background-color: white;" class=""><span lang="FR" style="font-size: 11pt; font-family: Calibri, sans-serif;" class="">Dear All,</span><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; background-color: white;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class="">The 10 nm ProteinA gold fiducials that we use in cryo-ET experiments strongly interact with one of our proteins of interest. Do you have any suggestions for a 10 or 6nm gold fiducials ?<o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; background-color: white;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class="">Best<o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; background-color: white;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class="">Daniel Lévy<o:p class=""></o:p></span></div></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div><div class="MsoNormal" align="center" style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif; text-align: center;"><hr size="2" width="100%" align="center" class=""></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 7.5pt; font-family: Arial, sans-serif; color: gray; background-color: white; background-position: initial initial; background-repeat: initial initial;" class=""><br class="">This e-mail message (including any attachments) is for the sole use of<br class="">the intended recipient(s) and may contain confidential and privileged<br class="">information. 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-webkit-text-stroke-width: 0px; float: none; display: inline !important;" class="">3dem mailing list</span><br style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><a href="mailto:3dem@ncmir.ucsd.edu" style="color: purple; text-decoration: underline; font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" class="">3dem@ncmir.ucsd.edu</a><br style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><a href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem" style="color: purple; text-decoration: underline; font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px;" class="">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</a></div></blockquote></div><br class=""><div class="">
<div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div style="color: rgb(0, 0, 0); letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div class=""><span style="orphans: 2; widows: 2;" class="">___________________________</span><br style="orphans: 2; widows: 2;" class=""><span style="orphans: 2; widows: 2;" class="">Université de Rennes 1</span><br style="orphans: 2; widows: 2;" class=""><span style="orphans: 2; widows: 2;" class="">UMR 6290, équipe TIPs</span><br style="orphans: 2; widows: 2;" class=""><span style="orphans: 2; widows: 2;" class="">Campus de Beaulieu, Bât. 13</span><br style="orphans: 2; widows: 2;" class=""><span style="orphans: 2; widows: 2;" class="">35042 Rennes Cedex</span><br style="orphans: 2; widows: 2;" class=""><span style="orphans: 2; widows: 2;" class="">France</span><br style="orphans: 2; widows: 2;" class=""><br style="orphans: 2; widows: 2;" class=""><span style="orphans: 2; widows: 2;" class="">Tel. +33 (0)2 23 23 67 64</span><br style="orphans: 2; widows: 2;" class=""><a href="http://equipes.igdr.univ-rennes1.fr/fr/tips/" style="orphans: 2; widows: 2;" class="">http://equipes.igdr.univ-rennes1.fr/en/tips/</a><br style="orphans: 2; widows: 2;" class=""><span style="orphans: 2; widows: 2;" class="">___________________________</span></div><div class=""><br class=""></div></div><br class="Apple-interchange-newline"></div><br class="Apple-interchange-newline"><br class="Apple-interchange-newline">
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