[3dem] gold fiducials

Sharon G Wolf Sharon.Wolf at weizmann.ac.il
Thu Sep 14 07:34:51 PDT 2017

Hi Daniel,
I can confirm that these gold fiducials  produced by Laurence Duchesne behave very nicely. We use them routinely here.
 They do not usually stick to macromolecular  or cell preps.

From: 3dem [mailto:3dem-bounces at ncmir.ucsd.edu] On Behalf Of dchretie
Sent: Thursday, September 14, 2017 16:35
To: 3dem at ncmir.ucsd.edu
Subject: Re: [3dem] gold fiducials

Dear Daniel,

We routinely use 10 nm gold NPs coated with a mixed PEG-peptidol matrix prepared according to :

Robust ligand shells for biological applications of gold nanoparticles
Duchesne L., Gentili D., Comes-Franchini M. and D.G. Fernig
Langmuir, 2008, 24:13572-13580

We rarely see interactions with proteins. They occasionally tend to stick to some types of lipid vesicles, notably at the air-water interfaces.

Best wishes,


Le 14 sept. 2017 à 14:35, Wright, Elizabeth R. <erwrigh at emory.edu<mailto:erwrigh at emory.edu>> a écrit :

Hi Daniel,

We use these…
BSA-treated tracers, EM grade (EMS, cat. no. 25486).
You can also make your own BSA-treated gold, protocols outlined in Iancu et al., Nature Protocols (2006).

Good luck!


Elizabeth R. Wright, PhD
Associate Professor
Emory University
(404) 727-4665
erwrigh at emory.edu<mailto:erwrigh at emory.edu>

From: 3dem <3dem-bounces at ncmir.ucsd.edu<mailto:3dem-bounces at ncmir.ucsd.edu>> on behalf of Levy Daniel <Daniel.Levy at curie.fr<mailto:Daniel.Levy at curie.fr>>
Date: Thursday, September 14, 2017 at 8:03 AM
To: "3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu>" <3dem at ncmir.ucsd.edu<mailto:3dem at ncmir.ucsd.edu>>
Subject: [3dem] gold fiducials

Dear All,
The 10 nm ProteinA gold fiducials that we use in cryo-ET experiments strongly interact with one of our proteins of interest. Do you have any suggestions for a 10 or 6nm gold fiducials ?
Daniel Lévy


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