<html xmlns:v="urn:schemas-microsoft-com:vml" xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:m="http://schemas.microsoft.com/office/2004/12/omml" xmlns="http://www.w3.org/TR/REC-html40">
<head>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
<meta name="Generator" content="Microsoft Word 15 (filtered medium)">
<!--[if !mso]><style>v\:* {behavior:url(#default#VML);}
o\:* {behavior:url(#default#VML);}
w\:* {behavior:url(#default#VML);}
.shape {behavior:url(#default#VML);}
</style><![endif]--><style><!--
/* Font Definitions */
@font-face
{font-family:Helvetica;
panose-1:2 11 6 4 2 2 2 2 2 4;}
@font-face
{font-family:"Cambria Math";
panose-1:2 4 5 3 5 4 6 3 2 4;}
@font-face
{font-family:Calibri;
panose-1:2 15 5 2 2 2 4 3 2 4;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{margin:0in;
margin-bottom:.0001pt;
font-size:12.0pt;
font-family:"Times New Roman",serif;}
a:link, span.MsoHyperlink
{mso-style-priority:99;
color:blue;
text-decoration:underline;}
a:visited, span.MsoHyperlinkFollowed
{mso-style-priority:99;
color:purple;
text-decoration:underline;}
span.bold
{mso-style-name:bold;}
span.italic
{mso-style-name:italic;}
span.apple-converted-space
{mso-style-name:apple-converted-space;}
span.EmailStyle20
{mso-style-type:personal-reply;
font-family:"Calibri",sans-serif;
color:#1F497D;}
.MsoChpDefault
{mso-style-type:export-only;
font-size:10.0pt;}
@page WordSection1
{size:8.5in 11.0in;
margin:1.0in 1.25in 1.0in 1.25in;}
div.WordSection1
{page:WordSection1;}
--></style><!--[if gte mso 9]><xml>
<o:shapedefaults v:ext="edit" spidmax="1026" />
</xml><![endif]--><!--[if gte mso 9]><xml>
<o:shapelayout v:ext="edit">
<o:idmap v:ext="edit" data="1" />
</o:shapelayout></xml><![endif]-->
</head>
<body lang="EN-US" link="blue" vlink="purple">
<div class="WordSection1">
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Hi Daniel,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">I can confirm that these gold fiducials produced by Laurence Duchesne behave very nicely. We use them routinely here.
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"> They do not usually stick to macromolecular or cell preps.
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Best,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Sharon<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><a name="_MailEndCompose"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></a></p>
<div>
<div style="border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0in 0in 0in">
<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">From:</span></b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> 3dem [mailto:3dem-bounces@ncmir.ucsd.edu]
<b>On Behalf Of </b>dchretie<br>
<b>Sent:</b> Thursday, September 14, 2017 16:35<br>
<b>To:</b> 3dem@ncmir.ucsd.edu<br>
<b>Subject:</b> Re: [3dem] gold fiducials<o:p></o:p></span></p>
</div>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Dear Daniel,<o:p></o:p></p>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<div>
<p class="MsoNormal">We routinely use 10 nm gold NPs coated with a mixed PEG-peptidol matrix prepared according to :<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<div>
<p class="MsoNormal"><span class="bold">Robust ligand shells for biological applications of gold nanoparticles</span>
<br>
<span class="bold">Duchesne L</span>., Gentili D., Comes-Franchini M. and D.G. Fernig<br>
<span class="italic">Langmuir</span>, 2008, 24:13572-13580<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<div>
<p class="MsoNormal">We rarely see interactions with proteins. They occasionally tend to stick to some types of lipid vesicles, notably at the air-water interfaces.<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<div>
<p class="MsoNormal">Best wishes,<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
<div>
<p class="MsoNormal">Denis<o:p></o:p></p>
</div>
<div>
<p class="MsoNormal"><o:p> </o:p></p>
<div>
<blockquote style="margin-top:5.0pt;margin-bottom:5.0pt">
<div>
<p class="MsoNormal">Le 14 sept. 2017 à 14:35, Wright, Elizabeth R. <<a href="mailto:erwrigh@emory.edu">erwrigh@emory.edu</a>> a écrit :<o:p></o:p></p>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
<div>
<div>
<p class="MsoNormal" style="background:white"><span style="font-family:"Arial",sans-serif">Hi Daniel,</span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal" style="background:white"><span style="font-family:"Arial",sans-serif"> </span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal" style="background:white"><span style="font-family:"Arial",sans-serif">We use these…</span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal" style="background:white"><span style="font-family:"Arial",sans-serif">BSA-treated tracers, EM grade (EMS, cat. no. 25486).</span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal" style="background:white"><span style="font-family:"Arial",sans-serif">You can also make your own BSA-treated gold, protocols outlined in Iancu et al., Nature Protocols (2006).</span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal" style="background:white"><span style="font-family:"Arial",sans-serif"> </span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal" style="background:white"><span style="font-family:"Arial",sans-serif">Good luck!</span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal" style="background:white"><span style="font-family:"Arial",sans-serif"> </span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal" style="background:white"><span style="font-family:"Arial",sans-serif">Liz</span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal" style="background:white"><span style="font-family:"Arial",sans-serif"> </span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
</div>
<div>
<div>
<p class="MsoNormal" style="background:white">----<span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal" style="background:white"><span style="font-family:"Arial",sans-serif">Elizabeth R. Wright, PhD</span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal" style="background:white"><span style="font-family:"Arial",sans-serif">Associate Professor</span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal" style="background:white"><span style="font-family:"Arial",sans-serif">Emory University</span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal" style="background:white"><span style="font-family:"Arial",sans-serif">(404) 727-4665</span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal" style="background:white"><span style="font-family:"Arial",sans-serif"><a href="mailto:erwrigh@emory.edu"><span style="color:purple">erwrigh@emory.edu</span></a></span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
</div>
</div>
<div>
<p class="MsoNormal" style="background:white"><span style="font-family:"Arial",sans-serif"> </span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal" style="background:white"><span style="font-family:"Arial",sans-serif"> </span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
</div>
<div style="border:none;border-top:solid #B5C4DF 1.0pt;padding:3.0pt 0in 0in 0in">
<div>
<p class="MsoNormal" style="background:white"><b><span style="font-family:"Calibri",sans-serif">From:<span class="apple-converted-space"> </span></span></b><span style="font-family:"Calibri",sans-serif">3dem <<a href="mailto:3dem-bounces@ncmir.ucsd.edu"><span style="color:purple">3dem-bounces@ncmir.ucsd.edu</span></a>>
on behalf of Levy Daniel <<a href="mailto:Daniel.Levy@curie.fr"><span style="color:purple">Daniel.Levy@curie.fr</span></a>><br>
<b>Date:<span class="apple-converted-space"> </span></b>Thursday, September 14, 2017 at 8:03 AM<br>
<b>To:<span class="apple-converted-space"> </span></b>"<a href="mailto:3dem@ncmir.ucsd.edu"><span style="color:purple">3dem@ncmir.ucsd.edu</span></a>" <<a href="mailto:3dem@ncmir.ucsd.edu"><span style="color:purple">3dem@ncmir.ucsd.edu</span></a>><br>
<b>Subject:<span class="apple-converted-space"> </span></b>[3dem] gold fiducials</span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
</div>
</div>
<div>
<div>
<p class="MsoNormal" style="background:white"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> <o:p></o:p></span></p>
</div>
</div>
<div>
<p class="MsoNormal" style="background:white"><span lang="FR" style="font-size:11.0pt;font-family:"Calibri",sans-serif">Dear All,</span><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"><o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal" style="background:white"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">The 10 nm ProteinA gold fiducials that we use in cryo-ET experiments strongly interact with one of our proteins of interest. Do you have any suggestions
for a 10 or 6nm gold fiducials ?<o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal" style="background:white"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">Best<o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal" style="background:white"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">Daniel Lévy<o:p></o:p></span></p>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
<div class="MsoNormal" align="center" style="text-align:center">
<hr size="2" width="100%" align="center">
</div>
<p class="MsoNormal"><span style="font-size:7.5pt;font-family:"Arial",sans-serif;color:gray;background:white"><br>
This e-mail message (including any attachments) is for the sole use of<br>
the intended recipient(s) and may contain confidential and privileged<br>
information. If the reader of this message is not the intended<br>
recipient, you are hereby notified that any dissemination, distribution<br>
or copying of this message (including any attachments) is strictly<br>
prohibited.<br>
<br>
If you have received this message in error, please contact<br>
the sender by reply e-mail message and destroy all copies of the<br>
original message (including attachments).<br>
</span><span style="font-size:9.0pt;font-family:"Helvetica",sans-serif;background:white">_______________________________________________</span><span style="font-size:9.0pt;font-family:"Helvetica",sans-serif"><br>
<span style="background:white">3dem mailing list</span><br>
</span><a href="mailto:3dem@ncmir.ucsd.edu"><span style="font-size:9.0pt;font-family:"Helvetica",sans-serif;color:purple;background:white">3dem@ncmir.ucsd.edu</span></a><span style="font-size:9.0pt;font-family:"Helvetica",sans-serif"><br>
</span><a href="https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem"><span style="font-size:9.0pt;font-family:"Helvetica",sans-serif;color:purple;background:white">https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem</span></a><o:p></o:p></p>
</div>
</blockquote>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
<div>
<div>
<div>
<div>
<p class="MsoNormal"><span style="color:black">___________________________<br>
Université de Rennes 1<br>
UMR 6290, équipe TIPs<br>
Campus de Beaulieu, Bât. 13<br>
35042 Rennes Cedex<br>
France<br>
<br>
Tel. +33 (0)2 23 23 67 64<br>
<a href="http://equipes.igdr.univ-rennes1.fr/fr/tips/">http://equipes.igdr.univ-rennes1.fr/en/tips/</a><br>
___________________________<o:p></o:p></span></p>
</div>
<div>
<p class="MsoNormal"><span style="color:black"><o:p> </o:p></span></p>
</div>
</div>
<p class="MsoNormal"><span style="color:black"><o:p> </o:p></span></p>
</div>
<p class="MsoNormal" style="margin-bottom:12.0pt"><o:p> </o:p></p>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
</div>
</div>
</body>
</html>