[3dem] One-sided vs two-sided blotting

Masahide Kikkawa mkikkawa at gmail.com
Thu Sep 14 20:35:25 PDT 2017

One of the advantage of one-sided blotting is the “filtering” effect of the holey carbon membrane for filamentous/tubular samples. 
In this case, you can use lower concentrations of samples, because you can “concentrate” samples on the grid.
Masahide Kikkawa, M.D., Ph. D.
Department of Cell Biology & Anatomy
Graduate School of Medicine
The University of Tokyo
tel: +81-3-5841-3338
fax: +81-3-5841-3339

> On Sep 15, 2017, at 12:31, Moni Banerjee <banerjee.moni at gmail.com> wrote:
> Dear all,
> I am wondering about the advantages (or disadvantages) of one-sided vs two-sided blotting during sample freezing for SPA. Do people have preferences for one or the other depending on the type of sample? Does one-sided blotting lead to thicker ice in general? Any suggestion/comment will be very welcome.
> Thanks,
> Manidipa
> -- 
> ------------------------
> Dr. Manidipa Banerjee, PhD
> Associate Professor
> School of Biological Sciences
> Indian Institute of Technology, Delhi
> New Delhi 110016
> India
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