[3dem] One-sided vs two-sided blotting

Moni Banerjee banerjee.moni at gmail.com
Thu Sep 14 20:31:53 PDT 2017

Dear all,

I am wondering about the advantages (or disadvantages) of one-sided vs
two-sided blotting during sample freezing for SPA. Do people have
preferences for one or the other depending on the type of sample? Does
one-sided blotting lead to thicker ice in general? Any suggestion/comment
will be very welcome.



Dr. Manidipa Banerjee, PhD
Associate Professor
School of Biological Sciences
Indian Institute of Technology, Delhi
New Delhi 110016
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