<html><head><meta http-equiv="Content-Type" content="text/html; charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;" class="">One of the advantage of one-sided blotting is the “filtering” effect of the holey carbon membrane for filamentous/tubular samples. <div class="">In this case, you can use lower concentrations of samples, because you can “concentrate” samples on the grid.<br class=""><div class="">
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word-spacing: 0px; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; " class=""><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px; color: rgb(0, 0, 0); font-family: Osaka; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; -webkit-text-decorations-in-effect: none; text-indent: 0px; -webkit-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px; color: rgb(0, 0, 0); font-family: Osaka; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; -webkit-text-decorations-in-effect: none; text-indent: 0px; -webkit-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px; color: rgb(0, 0, 0); font-family: Osaka; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; -webkit-text-decorations-in-effect: none; text-indent: 0px; -webkit-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px; color: rgb(0, 0, 0); font-family: Osaka; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; -webkit-text-decorations-in-effect: none; text-indent: 0px; -webkit-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px; color: rgb(0, 0, 0); font-family: Osaka; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; -webkit-text-decorations-in-effect: none; text-indent: 0px; -webkit-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px; color: rgb(0, 0, 0); font-family: Osaka; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; -webkit-text-decorations-in-effect: none; text-indent: 0px; -webkit-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><div class="">---------------------------------------------------------------</div><div class="">Masahide Kikkawa, M.D., Ph. D.</div><div class="">Professor</div><div class="">Department of Cell Biology & Anatomy</div><div class="">Graduate School of Medicine</div><div class="">The University of Tokyo</div><div class=""><a href="http://structure.m.u-tokyo.ac.jp/" class="">http://structure.m.u-tokyo.ac.jp/</a></div><div class="">tel: +81-3-5841-3338</div><div class=""><div class="">fax: +81-3-5841-3339</div><div class="">---------------------------------------------------------------</div></div><div class=""><br class=""></div></span></span></span></span></span></span></div></span></div></div></div></span></div></span><br class="Apple-interchange-newline"></div></span><br class="Apple-interchange-newline"></div></span><br class="Apple-interchange-newline"></div></span><br class="Apple-interchange-newline"></div></span><br class="Apple-interchange-newline"><br class="Apple-interchange-newline">
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<div><br class=""><blockquote type="cite" class=""><div class="">On Sep 15, 2017, at 12:31, Moni Banerjee <<a href="mailto:banerjee.moni@gmail.com" class="">banerjee.moni@gmail.com</a>> wrote:</div><br class="Apple-interchange-newline"><div class=""><div dir="ltr" class=""><p class="MsoNormal"><span lang="EN-US" class="">Dear all,<span class=""></span></span></p><div class=""><span lang="EN-US" class=""> </span><br class="webkit-block-placeholder"></div><p class="MsoNormal"><span lang="EN-US" class="">I am wondering about the advantages (or
disadvantages) of one-sided vs two-sided blotting during sample freezing for
SPA. Do people have preferences for one or the other depending on the type of sample?
Does one-sided blotting lead to thicker ice in general? Any suggestion/comment
will be very welcome. <span class=""></span></span></p><div class=""><span lang="EN-US" class=""> </span><br class="webkit-block-placeholder"></div><p class="MsoNormal"><span lang="EN-US" class="">Thanks,<span class=""></span></span></p><div class=""><span lang="EN-US" class=""> </span><br class="webkit-block-placeholder"></div><p class="MsoNormal"><span lang="EN-US" class="">Manidipa<span class=""></span></span></p>
<div class=""><br class=""></div>-- <br class=""><div class="gmail_signature"><div dir="ltr" class=""><div class="">------------------------<div class="">Dr. Manidipa Banerjee, PhD</div><div class="">Associate Professor</div><div class="">School of Biological Sciences</div><div class="">Indian Institute of Technology, Delhi</div><div class="">New Delhi 110016</div><div class="">India</div></div></div></div>
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