[3dem] K2 files

[Steven Ludtke]

A note about K2 DM4 files and EMAN2. Eric had tried EMAN2 to do his conversions, and it crashed on him, which is when he started trying other tools, which also seemed to be having problems. 

The current version of EMAN2 can read these files with no problems. Eric had been using a version from a pre-K2 release of EMAN2, which recognized DM4 files, but could not deal with some of the new things Gatan did when K2 support was added. 

ie - if you are trying to use EMAN2, please insure you have the latest version


> On Sep 21, 2016, at 11:34 PM, Eric Gilles Hanssen <ehanssen at unimelb.edu.au <mailto:ehanssen at unimelb.edu.au>> wrote:
> 
> ***CAUTION:*** This email is not from a BCM Source. Only click links or open attachments you know are safe.
> Little precisions:
> -  have tried imod dm2mrc. Not working for me <>
> - The image stacks are K2 dose fractionation for single particles, not sure if the format is the same then a tilt series for tomography.
>  
> Regards
> Eric
>  
>  
> -----------------
> Assoc. Prof Eric Hanssen
>  
> Head - Advanced Microscopy Facility
> Honorary Principle Fellow – Department of Biochemistry and Molecular Biology
> 
> Bio21 Molecular Science and Biotechnology Institute
> 30 Flemington Road - The University of Melbourne - Victoria 3010 - Australia
> email: ehanssen at unimelb.edu.au <mailto:ehanssen at unimelb.edu.au> | Office: +61 3 83442449 | Microscope: +61 3 83442509 
> Web: www.microscopy.unimelb.edu.au <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.unimelb.edu.au_&d=DQMFAg&c=ZQs-KZ8oxEw0p81sqgiaRA&r=Dk5VoQQ-wINYVssLMZihyC5Dj_sWYKxCyKz9E4Lp3gc&m=fvHYkIYnk2y67md52ljecI3Ho7fa-TBThjqXYczgEcI&s=DbLpkjdcRIRzYdZawa45lEVu8jAo9-cXWzAUZngItao&e=>
>  
>  
>  
> From: 3dem [mailto:3dem-bounces at ncmir.ucsd.edu <mailto:3dem-bounces at ncmir.ucsd.edu>] On Behalf Of Eric Gilles Hanssen
> Sent: Thursday, 22 September 2016 1:34 PM
> To: 3dem at ncmir.ucsd.edu <mailto:3dem at ncmir.ucsd.edu>
> Subject: [3dem] K2 files
>  
> Hi all, 
> I have a fair amount of .dm4 image stacks from a K2 camera. they are all too big (2.7GB) for my windows 32bit version of DM (yes i know I should upgrade to 64 bits, soon) . I would like to open these images with MOTIONCORR on my linux system.
> Is there any way of transforming the .dm4 in .mrc stacks without DM?
> regards
> Eric
>  
>  
> -----------------
> Assoc. Prof Eric Hanssen
>  
> Head - Advanced Microscopy Facility
> Honorary Principle Fellow – Department of Biochemistry and Molecular Biology
> 
> Bio21 Molecular Science and Biotechnology Institute
> 30 Flemington Road - The University of Melbourne - Victoria 3010 - Australia
> email: ehanssen at unimelb.edu.au <mailto:ehanssen at unimelb.edu.au> | Office: +61 3 83442449 | Microscope: +61 3 83442509 
> Web: www.microscopy.unimelb.edu.au <https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.unimelb.edu.au_&d=DQMFAg&c=ZQs-KZ8oxEw0p81sqgiaRA&r=Dk5VoQQ-wINYVssLMZihyC5Dj_sWYKxCyKz9E4Lp3gc&m=fvHYkIYnk2y67md52ljecI3Ho7fa-TBThjqXYczgEcI&s=DbLpkjdcRIRzYdZawa45lEVu8jAo9-cXWzAUZngItao&e=>
>  
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