[3dem] K2 files

James B jbouwer at ucsd.edu
Thu Sep 22 12:01:49 PDT 2016


Hi Mike,
Try dm2mrc() in the IMOD package.
-James

http://bio3d.colorado.edu/imod/doc/man/dm2mrc.html

James Bouwer, Ph.D.
Physicist - Manager Cryo-Electron Microscopy Facility
University of California, San Diego
Department of Chemistry and Biochemistry
Bonner Hall 1510 
9500 Gilman Drive
La Jolla, CA, 92093-0378
(858) 822-6718
jbouwer at ucsd.edu <mailto:jbouwer at ucsd.edu>



> On Sep 22, 2016, at 8:20 AM, Mike Strauss <mikestrauss13 at crystal.harvard.edu> wrote:
> 
> Have you tried the EMAN suite?  Maybe e2proc2d.py ?
> 
> mike
> 
> On Thu, Sep 22, 2016 at 9:57 AM, michael schatz <michael at imagescience.de <mailto:michael at imagescience.de>> wrote:
> Hi Eric,
> 
> Did you try em2em (https://www.imagescience.de/download.html#em2em <https://www.imagescience.de/download.html#em2em>)?
> But I am not sure if em2em can handle your type of DM4 images.
> 
> Regards
> Michael
> 
> ==
> 
> Eric Gilles Hanssen wrote:
>> Little precisions:
>> -  have tried imod dm2mrc. Not working for me <>
>> - The image stacks are K2 dose fractionation for single particles, not sure if the format is the same then a tilt series for tomography.
>> 
>>  
>> 
>> Regards
>> 
>> Eric
>> 
>>  
>> 
>> 
>>  
>> From: 3dem [mailto:3dem-bounces at ncmir.ucsd.edu <mailto:3dem-bounces at ncmir.ucsd.edu>] On Behalf Of Eric Gilles Hanssen
>> Sent: Thursday, 22 September 2016 1:34 PM
>> To: 3dem at ncmir.ucsd.edu <mailto:3dem at ncmir.ucsd.edu>
>> Subject: [3dem] K2 files
>> 
>>  
>> 
>> Hi all,
>> 
>> I have a fair amount of .dm4 image stacks from a K2 camera. they are all too big (2.7GB) for my windows 32bit version of DM (yes i know I should upgrade to 64 bits, soon) . I would like to open these images with MOTIONCORR on my linux system.
>> 
>> Is there any way of transforming the .dm4 in .mrc stacks without DM?
>> regards
>> 
>> Eric
>> 
> 
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