<html><head><meta http-equiv="Content-Type" content="text/html charset=utf-8"><meta http-equiv="Content-Type" content="text/html charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class=""><div class="">A note about K2 DM4 files and EMAN2. Eric had tried EMAN2 to do his conversions, and it crashed on him, which is when he started trying other tools, which also seemed to be having problems. </div><div class=""><br class=""></div><div class="">The current version of EMAN2 can read these files with no problems. Eric had been using a version from a pre-K2 release of EMAN2, which recognized DM4 files, but could not deal with some of the new things Gatan did when K2 support was added. </div><div class=""><br class=""></div><div class="">ie - if you are trying to use EMAN2, please insure you have the latest version</div><div class=""><br class=""></div><br class=""><div class=""><blockquote type="cite" class=""><div class="">On Sep 21, 2016, at 11:34 PM, Eric Gilles Hanssen <<a href="mailto:ehanssen@unimelb.edu.au" class="">ehanssen@unimelb.edu.au</a>> wrote:</div><br class="Apple-interchange-newline"><div class=""><font size="2" style="font-family: Helvetica; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><b class="">***CAUTION:*** This email is not from a BCM Source. Only click links or open attachments you know are safe.</b></font><span style="font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; float: none; display: inline !important;" class=""></span><hr style="font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><div class="WordSection1" style="page: WordSection1; font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;"><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><a name="_MailEndCompose" class=""><span style="color: rgb(31, 73, 125);" class="">Little precisions:<br class="">- have tried imod dm2mrc. Not working for me<o:p class=""></o:p></span></a></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="color: rgb(31, 73, 125);" class="">- The image stacks are K2 dose fractionation for single particles, not sure if the format is the same then a tilt series for tomography.<o:p class=""></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="color: rgb(31, 73, 125);" class=""><o:p class=""> </o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="color: rgb(31, 73, 125);" class="">Regards<o:p class=""></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="color: rgb(31, 73, 125);" class="">Eric<o:p class=""></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="color: rgb(31, 73, 125);" class=""><o:p class=""> </o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="color: rgb(31, 73, 125);" class=""><o:p class=""> </o:p></span></div><div class=""><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><b class=""><span style="font-size: 10pt; font-family: 'Comic Sans MS'; color: navy;" class="">-----------------<o:p class=""></o:p></span></b></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><b class=""><span style="font-size: 10pt; font-family: 'Comic Sans MS'; color: navy;" class="">Assoc. Prof Eric Hanssen<o:p class=""></o:p></span></b></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><b class=""><span style="font-size: 10pt; font-family: 'Comic Sans MS'; color: navy;" class=""><o:p class=""> </o:p></span></b></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><b class=""><span style="font-size: 10pt; font-family: 'Comic Sans MS'; color: rgb(31, 73, 125);" class="">Head - Advanced Microscopy Facility</span></b><b class=""><span style="font-size: 10pt; font-family: 'Comic Sans MS'; color: rgb(31, 73, 125);" class=""><o:p class=""></o:p></span></b></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><b class=""><span style="font-size: 10pt; font-family: 'Comic Sans MS'; color: rgb(31, 73, 125);" class="">Honorary Principle Fellow – Department of Biochemistry and Molecular Biology<o:p class=""></o:p></span></b></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 10pt; font-family: 'Comic Sans MS'; color: rgb(31, 73, 125);" class=""><br class="">Bio21 Molecular Science and Biotechnology Institute<br class=""></span><span style="font-size: 10pt; font-family: 'Comic Sans MS'; color: gray;" class="">30 Flemington Road - The University of Melbourne - Victoria 3010 - Australia<br class="">email:<span class="Apple-converted-space"> </span></span><a href="mailto:ehanssen@unimelb.edu.au" style="color: purple; text-decoration: underline;" class=""><span style="font-size: 10pt; font-family: 'Comic Sans MS'; color: gray;" class="">ehanssen@unimelb.edu.au</span></a><span style="font-size: 10pt; font-family: 'Comic Sans MS'; color: gray;" class=""><span class="Apple-converted-space"> </span>| Office: +61 3 83442449 | Microscope: +61 3 83442509<span class="Apple-converted-space"> </span><o:p class=""></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 10pt; font-family: 'Comic Sans MS'; color: gray;" class="">Web:<span class="Apple-converted-space"> </span></span><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.unimelb.edu.au_&d=DQMFAg&c=ZQs-KZ8oxEw0p81sqgiaRA&r=Dk5VoQQ-wINYVssLMZihyC5Dj_sWYKxCyKz9E4Lp3gc&m=fvHYkIYnk2y67md52ljecI3Ho7fa-TBThjqXYczgEcI&s=DbLpkjdcRIRzYdZawa45lEVu8jAo9-cXWzAUZngItao&e=" style="color: purple; text-decoration: underline;" class=""><span style="font-size: 10pt; font-family: 'Comic Sans MS'; color: blue;" class="">www.microscopy.unimelb.edu.au</span></a><span style="font-size: 10pt; font-family: 'Comic Sans MS'; color: gray;" class=""><o:p class=""></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="color: rgb(31, 73, 125);" class=""><o:p class=""> </o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="color: rgb(31, 73, 125);" class=""><o:p class=""> </o:p></span></div></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="color: rgb(31, 73, 125);" class=""><o:p class=""> </o:p></span></div><div class=""><div style="border-style: solid none none; border-top-color: rgb(181, 196, 223); border-top-width: 1pt; padding: 3pt 0cm 0cm;" class=""><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><b class=""><span lang="EN-US" style="font-size: 10pt; font-family: Tahoma, sans-serif;" class="">From:</span></b><span lang="EN-US" style="font-size: 10pt; font-family: Tahoma, sans-serif;" class=""><span class="Apple-converted-space"> </span>3dem [<a href="mailto:3dem-bounces@ncmir.ucsd.edu" style="color: purple; text-decoration: underline;" class="">mailto:3dem-bounces@ncmir.ucsd.edu</a>]<span class="Apple-converted-space"> </span><b class="">On Behalf Of<span class="Apple-converted-space"> </span></b>Eric Gilles Hanssen<br class=""><b class="">Sent:</b><span class="Apple-converted-space"> </span>Thursday, 22 September 2016 1:34 PM<br class=""><b class="">To:</b><span class="Apple-converted-space"> </span><a href="mailto:3dem@ncmir.ucsd.edu" style="color: purple; text-decoration: underline;" class="">3dem@ncmir.ucsd.edu</a><br class=""><b class="">Subject:</b><span class="Apple-converted-space"> </span>[3dem] K2 files<o:p class=""></o:p></span></div></div></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""> </o:p></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">Hi all,<span class="Apple-converted-space"> </span><o:p class=""></o:p></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">I have a fair amount of .dm4 image stacks from a K2 camera. they are all too big (2.7GB) for my windows 32bit version of DM (yes i know I should upgrade to 64 bits, soon) . I would like to open these images with MOTIONCORR on my linux system.<o:p class=""></o:p></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">Is there any way of transforming the .dm4 in .mrc stacks without DM?<br class="">regards<o:p class=""></o:p></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class="">Eric<o:p class=""></o:p></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""> </o:p></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""> </o:p></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><b class=""><span style="font-size: 10pt; font-family: 'Comic Sans MS'; color: navy;" class="">-----------------<o:p class=""></o:p></span></b></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><b class=""><span style="font-size: 10pt; font-family: 'Comic Sans MS'; color: navy;" class="">Assoc. Prof Eric Hanssen<o:p class=""></o:p></span></b></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><b class=""><span style="font-size: 10pt; font-family: 'Comic Sans MS'; color: navy;" class=""><o:p class=""> </o:p></span></b></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><b class=""><span style="font-size: 10pt; font-family: 'Comic Sans MS'; color: rgb(31, 73, 125);" class="">Head - Advanced Microscopy Facility<o:p class=""></o:p></span></b></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><b class=""><span style="font-size: 10pt; font-family: 'Comic Sans MS'; color: rgb(31, 73, 125);" class="">Honorary Principle Fellow – Department of Biochemistry and Molecular Biology<o:p class=""></o:p></span></b></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 10pt; font-family: 'Comic Sans MS'; color: rgb(31, 73, 125);" class=""><br class="">Bio21 Molecular Science and Biotechnology Institute<br class=""></span><span style="font-size: 10pt; font-family: 'Comic Sans MS'; color: gray;" class="">30 Flemington Road - The University of Melbourne - Victoria 3010 - Australia<br class="">email:<span class="Apple-converted-space"> </span></span><span style="color: rgb(31, 73, 125);" class=""><a href="mailto:ehanssen@unimelb.edu.au" style="color: purple; text-decoration: underline;" class=""><span style="font-size: 10pt; font-family: 'Comic Sans MS'; color: gray;" class="">ehanssen@unimelb.edu.au</span></a></span><span style="font-size: 10pt; font-family: 'Comic Sans MS'; color: gray;" class=""><span class="Apple-converted-space"> </span>| Office: +61 3 83442449 | Microscope: +61 3 83442509<span class="Apple-converted-space"> </span><o:p class=""></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span style="font-size: 10pt; font-family: 'Comic Sans MS'; color: gray;" class="">Web:<span class="Apple-converted-space"> </span><a href="https://urldefense.proofpoint.com/v2/url?u=http-3A__www.microscopy.unimelb.edu.au_&d=DQMFAg&c=ZQs-KZ8oxEw0p81sqgiaRA&r=Dk5VoQQ-wINYVssLMZihyC5Dj_sWYKxCyKz9E4Lp3gc&m=fvHYkIYnk2y67md52ljecI3Ho7fa-TBThjqXYczgEcI&s=DbLpkjdcRIRzYdZawa45lEVu8jAo9-cXWzAUZngItao&e=" style="color: purple; text-decoration: underline;" class="">www.microscopy.unimelb.edu.au</a><o:p class=""></o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span class=""><o:p class=""> </o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><span class=""><o:p class=""> </o:p></span></div><div style="margin: 0cm 0cm 0.0001pt; font-size: 11pt; font-family: Calibri, sans-serif;" class=""><o:p class=""> </o:p></div></div><span style="font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; float: none; display: inline !important;" class="">_______________________________________________</span><br style="font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><span style="font-family: Helvetica; font-size: 18px; font-style: normal; font-variant-caps: normal; font-weight: normal; letter-spacing: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; float: none; display: inline !important;" class="">3dem mailing list</span><br style="font-family: Helvetica; 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