[3dem] Leica EM GP

[Li Xing]

Hi

I have experience with both Vitrobot an Leica EM GP. I like the stereo
microscope in Leica EM GP as it helps a lot in optimizing the position and
the angle of filter paper for blotting. The freezing results is very
reproducible. I also like the baking precedure after each freezing as it
reduces water condensation in specimen chamber, that gives less
contamination on grid.  Leica EM GP can only do single-side blotting,
however, I don't see difference in compared to the double-side from
Vitrobot.

Hope this helps,

Li

On Sat, Jan 9, 2016 at 5:03 AM, De Carlo, Sacha <Sacha.DeCarlo at fei.com>
wrote:

> All,
>
>
>
> To forge an opinion based of facts, or stats, you may want to consult the
> EM Navigator (see screenshots if the moderators will let it through)… or go
> here:
>
> http://pdbj.org/emnavi/emnavi_result.php
>
>
>
> Then choose your favorite technique on the left side, then choose the List
> tab on the right hand side [Table radio button], and click ‘Instrument for
> Vitrification” option, as well as all other options you may be interested
> in. You can sort entries as you please, and see for yourself.
>
>
>
> Hope this helps.
>
>
>
> Best regards,
>
> Sacha
>
>
>
>
>
> *From:* 3dem [mailto:3dem-bounces at ncmir.ucsd.edu] *On Behalf Of *Ludovic
> Renault
> *Sent:* Saturday, January 09, 2016 10:35 AM
> *To:* David Belnap
> *Cc:* 3DEM Mailing List
> *Subject:* Re: [3dem] Leica EM GP
>
>
>
> Hi David,
>
> I have experience with manual plungers, cp3 and vitrobot.
>
> I find that cp3 is not as automated as the vitrobot and is thus much
> faster.
>
> You can freeze 4 grids very quickly.
>
> The pads can be home-made and the humidity is created with a sponge so it
> can't fail.
>
> The blotting time can be finely tuned (0.1 sec increments) and you can do
> double or single side blotting.
>
> Finally there's a ethane temperature monitor which I find very handy.
>
> That being said, I find the vitrobot freezing more reproducible. Once you
> have good freezing you'll likely get similar quality on all your grids.
> It's not the case with manual or cp3 in my experience.
>
> So overall I like cp3 for screening cryo-conditions. But I'm always
> nervous about the quality when I go collect data later on.
> I suppose cp3 is cheaper than a vitrobot (to be checked).
>
> I believe it must be possible to have all three models on loan for a
> couple of weeks to try them out?
>
> Sorry for hacking the initial question as I don't have experience with the
> Leica EM GP.
>
>
>
> Cheers,
>
> Ludo
>
> http://twitter.com/Ludo_cryoEM
>
>
>
> On Fri, Jan 8, 2016 at 11:49 PM, David Belnap <dbelnap at cores.utah.edu>
> wrote:
>
> Would people also add their experiences with the Gatan Cryoplunge 3?
> Knowing people’s experiences with all three current vitrification devices
> would be very helpful for those of us thinking about upgrading to one of
> the newer devices.
>
> Best wishes,
>
> David
>
> =======================================
> David M. Belnap
> Electron Microscopy Core Laboratory
> Department of Biochemistry
> Department of Biology
> 2501 SMBB
> University of Utah
> Salt Lake City, Utah 84112 USA
> Phone  801.585.1242
> FAX  801.587.3077
> David.Belnap at utah.edu
> =======================================
>
>
>
>
>
>
> > On Jan 8, 2016, at 1:45 PM, Edward Egelman <egelman at virginia.edu> wrote:
> >
> > Hi,
> >   Are there any updates on experience with this vitrification device?
> Has anyone found the stereo microscope attachment useful? Any comparisons
> with the FEI Vitrobot Mark IV would be helpful.
> > Regards,
> > Ed Egelman
> > --
> >
> >
> >
> > Edward H. Egelman, Ph.D.
> > Harrison Distinguished Professor
> > Dept. of Biochemistry and Molecular Genetics
> > University of Virginia
> >
> > President
> > Biophysical Society
> >
> > phone: 434-924-8210
> > fax: 434-924-5069
> > egelman at virginia.edu
> > http://www.people.virginia.edu/~ehe2n
> >
>
> > _______________________________________________
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> > 3dem at ncmir.ucsd.edu
> > https://mail.ncmir.ucsd.edu/mailman/listinfo/3dem
>
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