<div dir="ltr">Hi <div><br></div><div>I have experience with both Vitrobot an Leica EM GP. I like the stereo microscope in Leica EM GP as it helps a lot in optimizing the position and the angle of filter paper for blotting. The freezing results is very reproducible. I also like the baking precedure after each freezing as it reduces water condensation in specimen chamber, that gives less contamination on grid. Leica EM GP can only do single-side blotting, however, I don't see difference in compared to the double-side from Vitrobot.</div><div><br></div><div>Hope this helps,</div><div><br></div><div>Li</div></div><div class="gmail_extra"><br><div class="gmail_quote">On Sat, Jan 9, 2016 at 5:03 AM, De Carlo, Sacha <span dir="ltr"><<a href="mailto:Sacha.DeCarlo@fei.com" target="_blank">Sacha.DeCarlo@fei.com</a>></span> wrote:<br><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">
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<p class="MsoNormal"><span style="font-family:"Calibri","sans-serif";color:#1f497d">All,<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-family:"Calibri","sans-serif";color:#1f497d"><u></u> <u></u></span></p>
<p class="MsoNormal"><span style="font-family:"Calibri","sans-serif";color:#1f497d">To forge an opinion based of facts, or stats, you may want to consult the EM Navigator (see screenshots if the moderators will let it through)… or go here:<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-family:"Calibri","sans-serif";color:#1f497d"><a href="http://pdbj.org/emnavi/emnavi_result.php" target="_blank">http://pdbj.org/emnavi/emnavi_result.php</a><u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-family:"Calibri","sans-serif";color:#1f497d"><u></u> <u></u></span></p>
<p class="MsoNormal"><span style="font-family:"Calibri","sans-serif";color:#1f497d">Then choose your favorite technique on the left side, then choose the List tab on the right hand side [Table radio button], and click ‘Instrument for Vitrification” option,
as well as all other options you may be interested in. You can sort entries as you please, and see for yourself.<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-family:"Calibri","sans-serif";color:#1f497d"><u></u> <u></u></span></p>
<p class="MsoNormal"><span style="font-family:"Calibri","sans-serif";color:#1f497d">Hope this helps.<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-family:"Calibri","sans-serif";color:#1f497d"><u></u> <u></u></span></p>
<p class="MsoNormal"><span style="font-family:"Calibri","sans-serif";color:#1f497d">Best regards,<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-family:"Calibri","sans-serif";color:#1f497d">Sacha<u></u><u></u></span></p>
<p class="MsoNormal"><span style="font-family:"Calibri","sans-serif";color:#1f497d"><u></u> <u></u></span></p>
<p class="MsoNormal"><span style="font-family:"Calibri","sans-serif";color:#1f497d"><u></u> <u></u></span></p>
<p class="MsoNormal"><b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">From:</span></b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif""> 3dem [mailto:<a href="mailto:3dem-bounces@ncmir.ucsd.edu" target="_blank">3dem-bounces@ncmir.ucsd.edu</a>]
<b>On Behalf Of </b>Ludovic Renault<br>
<b>Sent:</b> Saturday, January 09, 2016 10:35 AM<br>
<b>To:</b> David Belnap<br>
<b>Cc:</b> 3DEM Mailing List<br>
<b>Subject:</b> Re: [3dem] Leica EM GP<u></u><u></u></span></p><div><div class="h5">
<p class="MsoNormal"><u></u> <u></u></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt"><span style="font-family:"Arial","sans-serif"">Hi David,<u></u><u></u></span></p>
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<p class="MsoNormal"><span style="font-family:"Arial","sans-serif"">I have experience with manual plungers, cp3 and vitrobot.<u></u><u></u></span></p>
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<p class="MsoNormal"><span style="font-family:"Arial","sans-serif"">I find that cp3 is not as automated as the vitrobot and is thus much faster.<u></u><u></u></span></p>
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<p class="MsoNormal"><span style="font-family:"Arial","sans-serif"">You can freeze 4 grids very quickly.<u></u><u></u></span></p>
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<p class="MsoNormal"><span style="font-family:"Arial","sans-serif"">The pads can be home-made and the humidity is created with a sponge so it can't fail.<u></u><u></u></span></p>
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<p class="MsoNormal"><span style="font-family:"Arial","sans-serif"">The blotting time can be finely tuned (0.1 sec increments) and you can do double or single side blotting.<u></u><u></u></span></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt"><span style="font-family:"Arial","sans-serif"">Finally there's a ethane temperature monitor which I find very handy.<u></u><u></u></span></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt"><span style="font-family:"Arial","sans-serif"">That being said, I find the vitrobot freezing more reproducible. Once you have good freezing you'll likely get similar quality on all your grids. It's not the case
with manual or cp3 in my experience.<u></u><u></u></span></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt"><span style="font-family:"Arial","sans-serif"">So overall I like cp3 for screening cryo-conditions. But I'm always nervous about the quality when I go collect data later on.
<br>
I suppose cp3 is cheaper than a vitrobot (to be checked).<u></u><u></u></span></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt"><span style="font-family:"Arial","sans-serif"">I believe it must be possible to have all three models on loan for a couple of weeks to try them out?<u></u><u></u></span></p>
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<p class="MsoNormal"><span style="font-family:"Arial","sans-serif"">Sorry for hacking the initial question as I don't have experience with the Leica EM GP.<u></u><u></u></span></p>
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<p class="MsoNormal"><span style="font-family:"Arial","sans-serif""><u></u> <u></u></span></p>
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<p class="MsoNormal">Cheers,<u></u><u></u></p>
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<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial","sans-serif";color:black">Ludo<br>
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<a href="http://twitter.com/Ludo_cryoEM" target="_blank">http://twitter.com/Ludo_cryoEM</a></span><u></u><u></u></p>
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<p class="MsoNormal"><u></u> <u></u></p>
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<p class="MsoNormal">On Fri, Jan 8, 2016 at 11:49 PM, David Belnap <<a href="mailto:dbelnap@cores.utah.edu" target="_blank">dbelnap@cores.utah.edu</a>> wrote:<u></u><u></u></p>
<p class="MsoNormal">Would people also add their experiences with the Gatan Cryoplunge 3? Knowing people’s experiences with all three current vitrification devices would be very helpful for those of us thinking about upgrading to one of the newer devices.<br>
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Best wishes,<br>
<br>
David<br>
<br>
=======================================<br>
David M. Belnap<br>
Electron Microscopy Core Laboratory<br>
Department of Biochemistry<br>
Department of Biology<br>
2501 SMBB<br>
University of Utah<br>
Salt Lake City, Utah 84112 USA<br>
Phone <a href="tel:801.585.1242" value="+18015851242" target="_blank">801.585.1242</a><br>
FAX <a href="tel:801.587.3077" value="+18015873077" target="_blank">801.587.3077</a><br>
<a href="mailto:David.Belnap@utah.edu" target="_blank">David.Belnap@utah.edu</a><br>
=======================================<u></u><u></u></p>
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> On Jan 8, 2016, at 1:45 PM, Edward Egelman <<a href="mailto:egelman@virginia.edu" target="_blank">egelman@virginia.edu</a>> wrote:<br>
><br>
> Hi,<br>
> Are there any updates on experience with this vitrification device? Has anyone found the stereo microscope attachment useful? Any comparisons with the FEI Vitrobot Mark IV would be helpful.<br>
> Regards,<br>
> Ed Egelman<br>
> --<br>
><br>
><br>
><br>
> Edward H. Egelman, Ph.D.<br>
> Harrison Distinguished Professor<br>
> Dept. of Biochemistry and Molecular Genetics<br>
> University of Virginia<br>
><br>
> President<br>
> Biophysical Society<br>
><br>
> phone: <a href="tel:434-924-8210" value="+14349248210" target="_blank">434-924-8210</a><br>
> fax: <a href="tel:434-924-5069" value="+14349245069" target="_blank">434-924-5069</a><br>
> <a href="mailto:egelman@virginia.edu" target="_blank">egelman@virginia.edu</a><br>
> <a href="http://www.people.virginia.edu/~ehe2n" target="_blank">http://www.people.virginia.edu/~ehe2n</a><br>
><u></u><u></u></p>
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