[3dem] Leica EM GP

[De Carlo, Sacha]

All,

To forge an opinion based of facts, or stats, you may want to consult the EM Navigator (see screenshots if the moderators will let it through)… or go here:
http://pdbj.org/emnavi/emnavi_result.php

Then choose your favorite technique on the left side, then choose the List tab on the right hand side [Table radio button], and click ‘Instrument for Vitrification” option, as well as all other options you may be interested in. You can sort entries as you please, and see for yourself.

Hope this helps.

Best regards,
Sacha


From: 3dem [mailto:3dem-bounces at ncmir.ucsd.edu] On Behalf Of Ludovic Renault
Sent: Saturday, January 09, 2016 10:35 AM
To: David Belnap
Cc: 3DEM Mailing List
Subject: Re: [3dem] Leica EM GP

Hi David,
I have experience with manual plungers, cp3 and vitrobot.
I find that cp3 is not as automated as the vitrobot and is thus much faster.
You can freeze 4 grids very quickly.
The pads can be home-made and the humidity is created with a sponge so it can't fail.
The blotting time can be finely tuned (0.1 sec increments) and you can do double or single side blotting.
Finally there's a ethane temperature monitor which I find very handy.
That being said, I find the vitrobot freezing more reproducible. Once you have good freezing you'll likely get similar quality on all your grids. It's not the case with manual or cp3 in my experience.
So overall I like cp3 for screening cryo-conditions. But I'm always nervous about the quality when I go collect data later on.
I suppose cp3 is cheaper than a vitrobot (to be checked).
I believe it must be possible to have all three models on loan for a couple of weeks to try them out?
Sorry for hacking the initial question as I don't have experience with the Leica EM GP.

Cheers,
Ludo

http://twitter.com/Ludo_cryoEM

On Fri, Jan 8, 2016 at 11:49 PM, David Belnap <dbelnap at cores.utah.edu<mailto:dbelnap at cores.utah.edu>> wrote:
Would people also add their experiences with the Gatan Cryoplunge 3?  Knowing people’s experiences with all three current vitrification devices would be very helpful for those of us thinking about upgrading to one of the newer devices.

Best wishes,

David

=======================================
David M. Belnap
Electron Microscopy Core Laboratory
Department of Biochemistry
Department of Biology
2501 SMBB
University of Utah
Salt Lake City, Utah 84112 USA
Phone  801.585.1242
FAX  801.587.3077
David.Belnap at utah.edu<mailto:David.Belnap at utah.edu>
=======================================





> On Jan 8, 2016, at 1:45 PM, Edward Egelman <egelman at virginia.edu<mailto:egelman at virginia.edu>> wrote:
>
> Hi,
>   Are there any updates on experience with this vitrification device? Has anyone found the stereo microscope attachment useful? Any comparisons with the FEI Vitrobot Mark IV would be helpful.
> Regards,
> Ed Egelman
> --
>
>
>
> Edward H. Egelman, Ph.D.
> Harrison Distinguished Professor
> Dept. of Biochemistry and Molecular Genetics
> University of Virginia
>
> President
> Biophysical Society
>
> phone: 434-924-8210
> fax: 434-924-5069
> egelman at virginia.edu<mailto:egelman at virginia.edu>
> http://www.people.virginia.edu/~ehe2n
>
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