[3dem] Leica EM GP

[Ludovic Renault]

Hi David,

I have experience with manual plungers, cp3 and vitrobot.
I find that cp3 is not as automated as the vitrobot and is thus much faster.
You can freeze 4 grids very quickly.
The pads can be home-made and the humidity is created with a sponge so it
can't fail.
The blotting time can be finely tuned (0.1 sec increments) and you can do
double or single side blotting.
Finally there's a ethane temperature monitor which I find very handy.

That being said, I find the vitrobot freezing more reproducible. Once you
have good freezing you'll likely get similar quality on all your grids.
It's not the case with manual or cp3 in my experience.

So overall I like cp3 for screening cryo-conditions. But I'm always nervous
about the quality when I go collect data later on.
I suppose cp3 is cheaper than a vitrobot (to be checked).

I believe it must be possible to have all three models on loan for a couple
of weeks to try them out?

Sorry for hacking the initial question as I don't have experience with the
Leica EM GP.

Cheers,
Ludo

http://twitter.com/Ludo_cryoEM

On Fri, Jan 8, 2016 at 11:49 PM, David Belnap <dbelnap at cores.utah.edu>
wrote:

> Would people also add their experiences with the Gatan Cryoplunge 3?
> Knowing people’s experiences with all three current vitrification devices
> would be very helpful for those of us thinking about upgrading to one of
> the newer devices.
>
> Best wishes,
>
> David
>
> =======================================
> David M. Belnap
> Electron Microscopy Core Laboratory
> Department of Biochemistry
> Department of Biology
> 2501 SMBB
> University of Utah
> Salt Lake City, Utah 84112 USA
> Phone  801.585.1242
> FAX  801.587.3077
> David.Belnap at utah.edu
> =======================================
>
>
>
>
>
> > On Jan 8, 2016, at 1:45 PM, Edward Egelman <egelman at virginia.edu> wrote:
> >
> > Hi,
> >   Are there any updates on experience with this vitrification device?
> Has anyone found the stereo microscope attachment useful? Any comparisons
> with the FEI Vitrobot Mark IV would be helpful.
> > Regards,
> > Ed Egelman
> > --
> >
> >
> >
> > Edward H. Egelman, Ph.D.
> > Harrison Distinguished Professor
> > Dept. of Biochemistry and Molecular Genetics
> > University of Virginia
> >
> > President
> > Biophysical Society
> >
> > phone: 434-924-8210
> > fax: 434-924-5069
> > egelman at virginia.edu
> > http://www.people.virginia.edu/~ehe2n
> >
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