[3dem] Leica EM GP

[Masahide Kikkawa]

Hi

We own Leica KF-80 and Leica EM GP and recently tried both Gatan Cryoplunge
3 and Vitrobot Mark IV.

Each machine has its own pros and cons. (here I omit KF-80 because
it wouldn't be available)

Leica EM GP:
pros:
* liquid ethane is temperature controlled. You don't need to keep watching.
* Blot from only one side. Therefore, you can "concentrate" a sample on
grid by the filtering effect if the sample is larger than the hole size. <-
For us, this is important.
* (in our experience) good for thick specimens.
cons:
* difficult to make thinner  and consistent thickness of ice.
* (probably our machine specific) temperature control for the chamber is
not so good.

To Ed: we don't usually use the stereo microscope, but as Li pointed out it
would be helpful as the blotting force is very sensitive to the changes of
forceps positions and need very careful optimisation.

Vitrobot Mark IV:
pros:
* easier to make thinner and and consistent thickness (or gradient) ice.
cons:
* ethane is not temperature controlled.

Gatan:
pros:
* simple
cons:
* not fully controlled.




On Sat, Jan 9, 2016 at 8:49 AM, David Belnap <dbelnap at cores.utah.edu> wrote:

> Would people also add their experiences with the Gatan Cryoplunge 3?
> Knowing people’s experiences with all three current vitrification devices
> would be very helpful for those of us thinking about upgrading to one of
> the newer devices.
>
> Best wishes,
>
> David
>
> =======================================
> David M. Belnap
> Electron Microscopy Core Laboratory
> Department of Biochemistry
> Department of Biology
> 2501 SMBB
> University of Utah
> Salt Lake City, Utah 84112 USA
> Phone  801.585.1242
> FAX  801.587.3077
> David.Belnap at utah.edu
> =======================================
>
>
>
>
>
> > On Jan 8, 2016, at 1:45 PM, Edward Egelman <egelman at virginia.edu> wrote:
> >
> > Hi,
> >   Are there any updates on experience with this vitrification device?
> Has anyone found the stereo microscope attachment useful? Any comparisons
> with the FEI Vitrobot Mark IV would be helpful.
> > Regards,
> > Ed Egelman
> > --
> >
> >
> >
> > Edward H. Egelman, Ph.D.
> > Harrison Distinguished Professor
> > Dept. of Biochemistry and Molecular Genetics
> > University of Virginia
> >
> > President
> > Biophysical Society
> >
> > phone: 434-924-8210
> > fax: 434-924-5069
> > egelman at virginia.edu
> > http://www.people.virginia.edu/~ehe2n
> >
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>
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-- 
---------------------------------------------------------------
吉川 雅英 (きっかわ まさひで）
東京大学・医学部・生体構造学 ・教授

tel 03-5841-3338
tel 03-5841-3339 (lab & fax)
〒113-0033 文京区本郷7-3-1
http://structure.m.u-tokyo.ac.jp/
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